Genetic variants of TSLP and asthma in an admixed urban population.

<h4>Background</h4>Thymic stromal lymphopoietin (TSLP), an IL7-like cytokine produced by bronchial epithelial cells is upregulated in asthma and induces dendritic cell maturation supporting a Th2 response. Environmental pollutants, including tobacco smoke and diesel exhaust particles upr...

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Main Authors: Mengling Liu, Linda Rogers, Qinyi Cheng, Yongzhao Shao, Maria Elena Fernandez-Beros, Joel N Hirschhorn, Helen N Lyon, Zofia K Z Gajdos, Sailaja Vedantam, Peter Gregersen, Michael F Seldin, Bertram Bleck, Adaikalavan Ramasamy, Anna-Liisa Hartikainen, Marjo-Riitta Jarvelin, Mikko Kuokkanen, Tarja Laitinen, Johan Eriksson, Terho Lehtimäki, Olli T Raitakari, Joan Reibman
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0025099&type=printable
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author Mengling Liu
Linda Rogers
Qinyi Cheng
Yongzhao Shao
Maria Elena Fernandez-Beros
Joel N Hirschhorn
Helen N Lyon
Zofia K Z Gajdos
Sailaja Vedantam
Peter Gregersen
Michael F Seldin
Bertram Bleck
Adaikalavan Ramasamy
Anna-Liisa Hartikainen
Marjo-Riitta Jarvelin
Mikko Kuokkanen
Tarja Laitinen
Johan Eriksson
Terho Lehtimäki
Olli T Raitakari
Joan Reibman
author_facet Mengling Liu
Linda Rogers
Qinyi Cheng
Yongzhao Shao
Maria Elena Fernandez-Beros
Joel N Hirschhorn
Helen N Lyon
Zofia K Z Gajdos
Sailaja Vedantam
Peter Gregersen
Michael F Seldin
Bertram Bleck
Adaikalavan Ramasamy
Anna-Liisa Hartikainen
Marjo-Riitta Jarvelin
Mikko Kuokkanen
Tarja Laitinen
Johan Eriksson
Terho Lehtimäki
Olli T Raitakari
Joan Reibman
author_sort Mengling Liu
collection DOAJ
description <h4>Background</h4>Thymic stromal lymphopoietin (TSLP), an IL7-like cytokine produced by bronchial epithelial cells is upregulated in asthma and induces dendritic cell maturation supporting a Th2 response. Environmental pollutants, including tobacco smoke and diesel exhaust particles upregulate TSLP suggesting that TSLP may be an interface between environmental pollution and immune responses in asthma. Since asthma is prevalent in urban communities, variants in the TSLP gene may be important in asthma susceptibility in these populations.<h4>Objectives</h4>To determine whether genetic variants in TSLP are associated with asthma in an urban admixed population. METHODOLOGY AND MAIN RESULTS: Ten tag-SNPs in the TSLP gene were analyzed for association with asthma using 387 clinically diagnosed asthmatic cases and 212 healthy controls from an urban admixed population. One SNP (rs1898671) showed nominally significant association with asthma (odds ratio (OR) = 1.50; 95% confidence interval (95% CI): 1.09-2.05, p = 0.01) after adjusting for age, BMI, income, education and population stratification. Association results were consistent using two different approaches to adjust for population stratification. When stratified by smoking status, the same SNP showed a significantly increased risk associated with asthma in ex-smokers (OR = 2.00, 95% CI: 1.04-3.83, p = 0.04) but not significant in never-smokers (OR = 1.34; 95% CI: 0.93-1.94, p = 0.11). Haplotype-specific score test indicated that an elevated risk for asthma was associated with a specific haplotype of TSLP involving SNP rs1898671 (OR = 1.58, 95% CI: 1.10-2.27, p = 0.01). Association of this SNP with asthma was confirmed in an independent large population-based cohort consortium study (OR = 1.15, 95% CI: 1.07-1.23, p = 0.0003) and the results stratified by smoking status were also validated (ex-smokers: OR = 1.21, 95% CI: 1.08-1.34, p = 0.003; never-smokers: OR = 1.06, 95% CI: 0.94-1.17, p = 0.33).<h4>Conclusions</h4>Genetic variants in TSLP may contribute to asthma susceptibility in admixed urban populations with a gene and environment interaction.
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spelling doaj-art-9d4d3904de424b6d82dfae2ac89c56d22025-08-20T02:08:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0169e2509910.1371/journal.pone.0025099Genetic variants of TSLP and asthma in an admixed urban population.Mengling LiuLinda RogersQinyi ChengYongzhao ShaoMaria Elena Fernandez-BerosJoel N HirschhornHelen N LyonZofia K Z GajdosSailaja VedantamPeter GregersenMichael F SeldinBertram BleckAdaikalavan RamasamyAnna-Liisa HartikainenMarjo-Riitta JarvelinMikko KuokkanenTarja LaitinenJohan ErikssonTerho LehtimäkiOlli T RaitakariJoan Reibman<h4>Background</h4>Thymic stromal lymphopoietin (TSLP), an IL7-like cytokine produced by bronchial epithelial cells is upregulated in asthma and induces dendritic cell maturation supporting a Th2 response. Environmental pollutants, including tobacco smoke and diesel exhaust particles upregulate TSLP suggesting that TSLP may be an interface between environmental pollution and immune responses in asthma. Since asthma is prevalent in urban communities, variants in the TSLP gene may be important in asthma susceptibility in these populations.<h4>Objectives</h4>To determine whether genetic variants in TSLP are associated with asthma in an urban admixed population. METHODOLOGY AND MAIN RESULTS: Ten tag-SNPs in the TSLP gene were analyzed for association with asthma using 387 clinically diagnosed asthmatic cases and 212 healthy controls from an urban admixed population. One SNP (rs1898671) showed nominally significant association with asthma (odds ratio (OR) = 1.50; 95% confidence interval (95% CI): 1.09-2.05, p = 0.01) after adjusting for age, BMI, income, education and population stratification. Association results were consistent using two different approaches to adjust for population stratification. When stratified by smoking status, the same SNP showed a significantly increased risk associated with asthma in ex-smokers (OR = 2.00, 95% CI: 1.04-3.83, p = 0.04) but not significant in never-smokers (OR = 1.34; 95% CI: 0.93-1.94, p = 0.11). Haplotype-specific score test indicated that an elevated risk for asthma was associated with a specific haplotype of TSLP involving SNP rs1898671 (OR = 1.58, 95% CI: 1.10-2.27, p = 0.01). Association of this SNP with asthma was confirmed in an independent large population-based cohort consortium study (OR = 1.15, 95% CI: 1.07-1.23, p = 0.0003) and the results stratified by smoking status were also validated (ex-smokers: OR = 1.21, 95% CI: 1.08-1.34, p = 0.003; never-smokers: OR = 1.06, 95% CI: 0.94-1.17, p = 0.33).<h4>Conclusions</h4>Genetic variants in TSLP may contribute to asthma susceptibility in admixed urban populations with a gene and environment interaction.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0025099&type=printable
spellingShingle Mengling Liu
Linda Rogers
Qinyi Cheng
Yongzhao Shao
Maria Elena Fernandez-Beros
Joel N Hirschhorn
Helen N Lyon
Zofia K Z Gajdos
Sailaja Vedantam
Peter Gregersen
Michael F Seldin
Bertram Bleck
Adaikalavan Ramasamy
Anna-Liisa Hartikainen
Marjo-Riitta Jarvelin
Mikko Kuokkanen
Tarja Laitinen
Johan Eriksson
Terho Lehtimäki
Olli T Raitakari
Joan Reibman
Genetic variants of TSLP and asthma in an admixed urban population.
PLoS ONE
title Genetic variants of TSLP and asthma in an admixed urban population.
title_full Genetic variants of TSLP and asthma in an admixed urban population.
title_fullStr Genetic variants of TSLP and asthma in an admixed urban population.
title_full_unstemmed Genetic variants of TSLP and asthma in an admixed urban population.
title_short Genetic variants of TSLP and asthma in an admixed urban population.
title_sort genetic variants of tslp and asthma in an admixed urban population
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0025099&type=printable
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