Evaluation of the safety, immunogenicity and efficacy of a new live-attenuated lumpy skin disease vaccine in India

Lumpy skin disease (LSD) was reported for the first time in India in 2019 and since then, it has become endemic. Since a homologous (LSD-virus based) vaccine was not available in the country, goatpox virus (GPV)-based heterologous vaccine was authorized for mass immunization to induce protection aga...

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Main Authors: Naveen Kumar, Sanjay Barua, Ram Kumar, Nitin Khandelwal, Amit Kumar, Assim Verma, Lokender Singh, Bhagraj Godara, Yogesh Chander, Garvit Kumar, Thachamvally Riyesh, Deepak Kumar Sharma, Anubha Pathak, Sanjay Kumar, Ramesh Kumar Dedar, Vishal Mehta, Mitesh Gaur, Bhupendra Bhardwaj, Vithilesh Vyas, Sarjeet Chaudhary, Vijaypal Yadav, Adrish Bhati, Rakesh Kaul, Arif Bashir, Anjum Andrabi, Raja Wasim Yousuf, Abhimanyu Koul, Subhash Kachhawaha, Amol Gurav, Siddharth Gautam, Hari Audh Tiwari, Vijay Kumar Munjal, Madhurendu K. Gupta, Rajender Kumar, Baldev R. Gulati, Jyoti Misri, Ashok Kumar, Ashok Kumar Mohanty, Sukdeb Nandi, Karam Pal Singh, Yash Pal, Triveni Dutt, Bhupendra N. Tripathi
Format: Article
Language:English
Published: Taylor & Francis Group 2023-12-01
Series:Virulence
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Online Access:https://www.tandfonline.com/doi/10.1080/21505594.2023.2190647
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author Naveen Kumar
Sanjay Barua
Ram Kumar
Nitin Khandelwal
Amit Kumar
Assim Verma
Lokender Singh
Bhagraj Godara
Yogesh Chander
Garvit Kumar
Thachamvally Riyesh
Deepak Kumar Sharma
Anubha Pathak
Sanjay Kumar
Ramesh Kumar Dedar
Vishal Mehta
Mitesh Gaur
Bhupendra Bhardwaj
Vithilesh Vyas
Sarjeet Chaudhary
Vijaypal Yadav
Adrish Bhati
Rakesh Kaul
Arif Bashir
Anjum Andrabi
Raja Wasim Yousuf
Abhimanyu Koul
Subhash Kachhawaha
Amol Gurav
Siddharth Gautam
Hari Audh Tiwari
Vijay Kumar Munjal
Madhurendu K. Gupta
Rajender Kumar
Baldev R. Gulati
Jyoti Misri
Ashok Kumar
Ashok Kumar Mohanty
Sukdeb Nandi
Karam Pal Singh
Yash Pal
Triveni Dutt
Bhupendra N. Tripathi
author_facet Naveen Kumar
Sanjay Barua
Ram Kumar
Nitin Khandelwal
Amit Kumar
Assim Verma
Lokender Singh
Bhagraj Godara
Yogesh Chander
Garvit Kumar
Thachamvally Riyesh
Deepak Kumar Sharma
Anubha Pathak
Sanjay Kumar
Ramesh Kumar Dedar
Vishal Mehta
Mitesh Gaur
Bhupendra Bhardwaj
Vithilesh Vyas
Sarjeet Chaudhary
Vijaypal Yadav
Adrish Bhati
Rakesh Kaul
Arif Bashir
Anjum Andrabi
Raja Wasim Yousuf
Abhimanyu Koul
Subhash Kachhawaha
Amol Gurav
Siddharth Gautam
Hari Audh Tiwari
Vijay Kumar Munjal
Madhurendu K. Gupta
Rajender Kumar
Baldev R. Gulati
Jyoti Misri
Ashok Kumar
Ashok Kumar Mohanty
Sukdeb Nandi
Karam Pal Singh
Yash Pal
Triveni Dutt
Bhupendra N. Tripathi
author_sort Naveen Kumar
collection DOAJ
description Lumpy skin disease (LSD) was reported for the first time in India in 2019 and since then, it has become endemic. Since a homologous (LSD-virus based) vaccine was not available in the country, goatpox virus (GPV)-based heterologous vaccine was authorized for mass immunization to induce protection against LSD in cattle. This study describes the evaluation of safety, immunogenicity and efficacy of a new live-attenuated LSD vaccine developed by using an Indian field strain, isolated in 2019 from cattle. The virus was attenuated by continuous passage (P = 50) in Vero cells. The vaccine (50th LSDV passage in Vero cells, named as Lumpi-ProVacInd) did not induce any local or systemic reaction upon its experimental inoculation in calves (n = 10). At day 30 post-vaccination (pv), the vaccinated animals were shown to develop antibody- and cell-mediated immune responses and exhibited complete protection upon virulent LSDV challenge. A minimum Neethling response (0.018% animals; 5 out of 26,940 animals) of the vaccine was observed in the field trials conducted in 26,940 animals. There was no significant reduction in the milk yield in lactating animals (n = 10108), besides there was no abortion or any other reproductive disorder in the pregnant animals (n = 2889). Sero-conversion was observed in 85.18% animals in the field by day 30 pv.
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spelling doaj-art-9d4d113d60dd47fbb05dfcaf60a677c42025-08-20T02:38:15ZengTaylor & Francis GroupVirulence2150-55942150-56082023-12-0114110.1080/21505594.2023.2190647Evaluation of the safety, immunogenicity and efficacy of a new live-attenuated lumpy skin disease vaccine in IndiaNaveen Kumar0Sanjay Barua1Ram Kumar2Nitin Khandelwal3Amit Kumar4Assim Verma5Lokender Singh6Bhagraj Godara7Yogesh Chander8Garvit Kumar9Thachamvally Riyesh10Deepak Kumar Sharma11Anubha Pathak12Sanjay Kumar13Ramesh Kumar Dedar14Vishal Mehta15Mitesh Gaur16Bhupendra Bhardwaj17Vithilesh Vyas18Sarjeet Chaudhary19Vijaypal Yadav20Adrish Bhati21Rakesh Kaul22Arif Bashir23Anjum Andrabi24Raja Wasim Yousuf25Abhimanyu Koul26Subhash Kachhawaha27Amol Gurav28Siddharth Gautam29Hari Audh Tiwari30Vijay Kumar Munjal31Madhurendu K. Gupta32Rajender Kumar33Baldev R. Gulati34Jyoti Misri35Ashok Kumar36Ashok Kumar Mohanty37Sukdeb Nandi38Karam Pal Singh39Yash Pal40Triveni Dutt41Bhupendra N. Tripathi42National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaIndian Veterinary Research Institute, Mukteswar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaDepartment of Veterinary Microbiology, College of Veterinary and Animal Science, Udaipur, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaDepartment of Animal Husbandry, Banswara, Rajasthan, IndiaDepartment of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Udaipur, IndiaDepartment of Animal Husbandry, Udaipur, Rajasthan, IndiaDepartment of Animal Husbandry, Jodhpur, Rajasthan, IndiaDepartment of Animal Husbandry, Alwar, Rajasthan, IndiaDepartment of Animal Husbandry, Alwar, Rajasthan, IndiaLivestock Research station, Nohar, Rajasthan, IndiaAnimal Husbandry Department, Jammu and Kashmir, IndiaAnimal Husbandry Department, Jammu and Kashmir, IndiaAnimal Husbandry Department, Jammu and Kashmir, IndiaAnimal Husbandry Department, Jammu and Kashmir, IndiaAnimal Husbandry Department, Jammu and Kashmir, IndiaKrishi Vigyan Kendra, ICAR-Central Arid Zone Research Institute, Jodhpur, IndiaIndian Veterinary Research Institute, Mukteswar, IndiaIndian Veterinary Research Institute, Mukteswar, IndiaHasanand Gaushala, Vrindavan, Mathura, IndiaBovine Sperm Station and Research Centre, Hisar, IndiaDepartment of Veterinary Pathology, Birsa Agricultural University, Ranchi, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaAnimal Science Division, Indian Council of Agricultural Research, IndiaAnimal Science Division, Indian Council of Agricultural Research, IndiaIndian Veterinary Research Institute, Mukteswar, IndiaCentre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, IndiaCentre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaCentre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, IndiaNational Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, IndiaLumpy skin disease (LSD) was reported for the first time in India in 2019 and since then, it has become endemic. Since a homologous (LSD-virus based) vaccine was not available in the country, goatpox virus (GPV)-based heterologous vaccine was authorized for mass immunization to induce protection against LSD in cattle. This study describes the evaluation of safety, immunogenicity and efficacy of a new live-attenuated LSD vaccine developed by using an Indian field strain, isolated in 2019 from cattle. The virus was attenuated by continuous passage (P = 50) in Vero cells. The vaccine (50th LSDV passage in Vero cells, named as Lumpi-ProVacInd) did not induce any local or systemic reaction upon its experimental inoculation in calves (n = 10). At day 30 post-vaccination (pv), the vaccinated animals were shown to develop antibody- and cell-mediated immune responses and exhibited complete protection upon virulent LSDV challenge. A minimum Neethling response (0.018% animals; 5 out of 26,940 animals) of the vaccine was observed in the field trials conducted in 26,940 animals. There was no significant reduction in the milk yield in lactating animals (n = 10108), besides there was no abortion or any other reproductive disorder in the pregnant animals (n = 2889). Sero-conversion was observed in 85.18% animals in the field by day 30 pv.https://www.tandfonline.com/doi/10.1080/21505594.2023.2190647Lumpy skin diseaseLSD/RanchiIndiahomologous live-attenuated LSD vaccine
spellingShingle Naveen Kumar
Sanjay Barua
Ram Kumar
Nitin Khandelwal
Amit Kumar
Assim Verma
Lokender Singh
Bhagraj Godara
Yogesh Chander
Garvit Kumar
Thachamvally Riyesh
Deepak Kumar Sharma
Anubha Pathak
Sanjay Kumar
Ramesh Kumar Dedar
Vishal Mehta
Mitesh Gaur
Bhupendra Bhardwaj
Vithilesh Vyas
Sarjeet Chaudhary
Vijaypal Yadav
Adrish Bhati
Rakesh Kaul
Arif Bashir
Anjum Andrabi
Raja Wasim Yousuf
Abhimanyu Koul
Subhash Kachhawaha
Amol Gurav
Siddharth Gautam
Hari Audh Tiwari
Vijay Kumar Munjal
Madhurendu K. Gupta
Rajender Kumar
Baldev R. Gulati
Jyoti Misri
Ashok Kumar
Ashok Kumar Mohanty
Sukdeb Nandi
Karam Pal Singh
Yash Pal
Triveni Dutt
Bhupendra N. Tripathi
Evaluation of the safety, immunogenicity and efficacy of a new live-attenuated lumpy skin disease vaccine in India
Virulence
Lumpy skin disease
LSD/Ranchi
India
homologous live-attenuated LSD vaccine
title Evaluation of the safety, immunogenicity and efficacy of a new live-attenuated lumpy skin disease vaccine in India
title_full Evaluation of the safety, immunogenicity and efficacy of a new live-attenuated lumpy skin disease vaccine in India
title_fullStr Evaluation of the safety, immunogenicity and efficacy of a new live-attenuated lumpy skin disease vaccine in India
title_full_unstemmed Evaluation of the safety, immunogenicity and efficacy of a new live-attenuated lumpy skin disease vaccine in India
title_short Evaluation of the safety, immunogenicity and efficacy of a new live-attenuated lumpy skin disease vaccine in India
title_sort evaluation of the safety immunogenicity and efficacy of a new live attenuated lumpy skin disease vaccine in india
topic Lumpy skin disease
LSD/Ranchi
India
homologous live-attenuated LSD vaccine
url https://www.tandfonline.com/doi/10.1080/21505594.2023.2190647
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