The GAGA factor regulatory network: Identification of GAGA factor associated proteins.
The Drosophila GAGA factor (GAF) has an extraordinarily diverse set of functions that include the activation and silencing of gene expression, nucleosome organization and remodeling, higher order chromosome architecture and mitosis. One hypothesis that could account for these diverse activities is t...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0173602 |
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| author | Dmitry Lomaev Anna Mikhailova Maksim Erokhin Alexander V Shaposhnikov James J Moresco Tatiana Blokhina Daniel Wolle Tsutomu Aoki Vladimir Ryabykh John R Yates Yulii V Shidlovskii Pavel Georgiev Paul Schedl Darya Chetverina |
| author_facet | Dmitry Lomaev Anna Mikhailova Maksim Erokhin Alexander V Shaposhnikov James J Moresco Tatiana Blokhina Daniel Wolle Tsutomu Aoki Vladimir Ryabykh John R Yates Yulii V Shidlovskii Pavel Georgiev Paul Schedl Darya Chetverina |
| author_sort | Dmitry Lomaev |
| collection | DOAJ |
| description | The Drosophila GAGA factor (GAF) has an extraordinarily diverse set of functions that include the activation and silencing of gene expression, nucleosome organization and remodeling, higher order chromosome architecture and mitosis. One hypothesis that could account for these diverse activities is that GAF is able to interact with partners that have specific and dedicated functions. To test this possibility we used affinity purification coupled with high throughput mass spectrometry to identify GAF associated partners. Consistent with this hypothesis the GAF interacting network includes a large collection of factors and complexes that have been implicated in many different aspects of gene activity, chromosome structure and function. Moreover, we show that GAF interactions with a small subset of partners is direct; however for many others the interactions could be indirect, and depend upon intermediates that serve to diversify the functional capabilities of the GAF protein. |
| format | Article |
| id | doaj-art-9d4ccebe7414442dae7be62cf0c40de0 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-9d4ccebe7414442dae7be62cf0c40de02025-08-20T02:20:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017360210.1371/journal.pone.0173602The GAGA factor regulatory network: Identification of GAGA factor associated proteins.Dmitry LomaevAnna MikhailovaMaksim ErokhinAlexander V ShaposhnikovJames J MorescoTatiana BlokhinaDaniel WolleTsutomu AokiVladimir RyabykhJohn R YatesYulii V ShidlovskiiPavel GeorgievPaul SchedlDarya ChetverinaThe Drosophila GAGA factor (GAF) has an extraordinarily diverse set of functions that include the activation and silencing of gene expression, nucleosome organization and remodeling, higher order chromosome architecture and mitosis. One hypothesis that could account for these diverse activities is that GAF is able to interact with partners that have specific and dedicated functions. To test this possibility we used affinity purification coupled with high throughput mass spectrometry to identify GAF associated partners. Consistent with this hypothesis the GAF interacting network includes a large collection of factors and complexes that have been implicated in many different aspects of gene activity, chromosome structure and function. Moreover, we show that GAF interactions with a small subset of partners is direct; however for many others the interactions could be indirect, and depend upon intermediates that serve to diversify the functional capabilities of the GAF protein.https://doi.org/10.1371/journal.pone.0173602 |
| spellingShingle | Dmitry Lomaev Anna Mikhailova Maksim Erokhin Alexander V Shaposhnikov James J Moresco Tatiana Blokhina Daniel Wolle Tsutomu Aoki Vladimir Ryabykh John R Yates Yulii V Shidlovskii Pavel Georgiev Paul Schedl Darya Chetverina The GAGA factor regulatory network: Identification of GAGA factor associated proteins. PLoS ONE |
| title | The GAGA factor regulatory network: Identification of GAGA factor associated proteins. |
| title_full | The GAGA factor regulatory network: Identification of GAGA factor associated proteins. |
| title_fullStr | The GAGA factor regulatory network: Identification of GAGA factor associated proteins. |
| title_full_unstemmed | The GAGA factor regulatory network: Identification of GAGA factor associated proteins. |
| title_short | The GAGA factor regulatory network: Identification of GAGA factor associated proteins. |
| title_sort | gaga factor regulatory network identification of gaga factor associated proteins |
| url | https://doi.org/10.1371/journal.pone.0173602 |
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