Association of CYP2D6*4 Polymorphism With Response to Atorvastatin in Patients With High Low-Density Lipoprotein Level in Northern Iran, Guilan Provice
Background: People who take statins respond differently to them due to genetic differences. One of the most significant enzymes involved in drug metabolism is cytochrome P450 2D6 (CYP2D6) enzyme, coded by the CYP2D6 gene. Individuals who carry two non-functional alleles in this gene are considered a...
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| Format: | Article |
| Language: | fas |
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Guilan University of Medical Sciences
2023-04-01
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| Series: | مجله دانشگاه علوم پزشکی گیلان |
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| Online Access: | http://journal.gums.ac.ir/article-1-2552-en.pdf |
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| author | Raziyeh Asadollahpour Faeze Khaghani Fardin Mirbolouk Anvarsadat Kianmehr Omid Goodarzvand Sara Dabirian Mohammad Sadegh Alipour Ehsan Zamani Mehdi Evazalipour |
| author_facet | Raziyeh Asadollahpour Faeze Khaghani Fardin Mirbolouk Anvarsadat Kianmehr Omid Goodarzvand Sara Dabirian Mohammad Sadegh Alipour Ehsan Zamani Mehdi Evazalipour |
| author_sort | Raziyeh Asadollahpour |
| collection | DOAJ |
| description | Background: People who take statins respond differently to them due to genetic differences. One of the most significant enzymes involved in drug metabolism is cytochrome P450 2D6 (CYP2D6) enzyme, coded by the CYP2D6 gene. Individuals who carry two non-functional alleles in this gene are considered as poor metabolizers. Recognizing poor metabolizers may help prevent adverse effects of drugs.
Objective: This study aims to assess the association of CYP2D6*4, as the most frequent non-functional allele of CYP2D6 gene, and response to atorvastatin in patients with high low-density lipoprotein (LDL) level in northern Iran.
Methods: A total of 180 patients with high LDL level underwent treatment with atorvastatin for 8 weeks to assess their response. They were assessed in terms of CYP2D6*4 polymorphism using the amplification-refractory mutation system/polymerase chain reaction method and the results were validated by the sanger sequencing method. At the end, the association between CYP2D6*4 allele and response to atorvastatin was assessed.
Results: In patients, the percentage of the CYP2D6*4 variant was 7%. This allele was not observed in homozygous patients. There was no significant association between CYP2D6*4 polymorphism and response to atorvastatin, which might be due to low frequency of CYP2D6*4 in patients. The observed allelic frequency was close to the frequency reported in previous studies for healthy Iranian people.
Conclusion: It seems that CYP2D6*4 polymorphism is not the cause of poor metabolism in poor metabolizers in northern Iran. Therefore, to diagnose poor metabolizers in this region, further studies on other genes are recommended. |
| format | Article |
| id | doaj-art-9d41b9ca347a469a941f296d044eb03b |
| institution | OA Journals |
| issn | 2008-4048 2008-4056 |
| language | fas |
| publishDate | 2023-04-01 |
| publisher | Guilan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گیلان |
| spelling | doaj-art-9d41b9ca347a469a941f296d044eb03b2025-08-20T01:53:11ZfasGuilan University of Medical Sciencesمجله دانشگاه علوم پزشکی گیلان2008-40482008-40562023-04-013214053Association of CYP2D6*4 Polymorphism With Response to Atorvastatin in Patients With High Low-Density Lipoprotein Level in Northern Iran, Guilan ProviceRaziyeh Asadollahpour0Faeze Khaghani1Fardin Mirbolouk2Anvarsadat Kianmehr3Omid Goodarzvand4Sara Dabirian5Mohammad Sadegh Alipour6Ehsan Zamani7Mehdi Evazalipour8 Student Research Committee, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran. Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran. Cardiovascular Diseases Research Center, Department of Cardiology Heshmat Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran. Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran. Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran. Statistical Research and Training Center, Statistical Center of Iran, Tehran, Iran. Department of Pharmacology and Toxicology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran. Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran. Background: People who take statins respond differently to them due to genetic differences. One of the most significant enzymes involved in drug metabolism is cytochrome P450 2D6 (CYP2D6) enzyme, coded by the CYP2D6 gene. Individuals who carry two non-functional alleles in this gene are considered as poor metabolizers. Recognizing poor metabolizers may help prevent adverse effects of drugs. Objective: This study aims to assess the association of CYP2D6*4, as the most frequent non-functional allele of CYP2D6 gene, and response to atorvastatin in patients with high low-density lipoprotein (LDL) level in northern Iran. Methods: A total of 180 patients with high LDL level underwent treatment with atorvastatin for 8 weeks to assess their response. They were assessed in terms of CYP2D6*4 polymorphism using the amplification-refractory mutation system/polymerase chain reaction method and the results were validated by the sanger sequencing method. At the end, the association between CYP2D6*4 allele and response to atorvastatin was assessed. Results: In patients, the percentage of the CYP2D6*4 variant was 7%. This allele was not observed in homozygous patients. There was no significant association between CYP2D6*4 polymorphism and response to atorvastatin, which might be due to low frequency of CYP2D6*4 in patients. The observed allelic frequency was close to the frequency reported in previous studies for healthy Iranian people. Conclusion: It seems that CYP2D6*4 polymorphism is not the cause of poor metabolism in poor metabolizers in northern Iran. Therefore, to diagnose poor metabolizers in this region, further studies on other genes are recommended.http://journal.gums.ac.ir/article-1-2552-en.pdfatorvastatincyp2d6polymorphismldl |
| spellingShingle | Raziyeh Asadollahpour Faeze Khaghani Fardin Mirbolouk Anvarsadat Kianmehr Omid Goodarzvand Sara Dabirian Mohammad Sadegh Alipour Ehsan Zamani Mehdi Evazalipour Association of CYP2D6*4 Polymorphism With Response to Atorvastatin in Patients With High Low-Density Lipoprotein Level in Northern Iran, Guilan Provice مجله دانشگاه علوم پزشکی گیلان atorvastatin cyp2d6 polymorphism ldl |
| title | Association of CYP2D6*4 Polymorphism With Response to Atorvastatin in Patients With High Low-Density Lipoprotein Level in Northern Iran, Guilan Provice |
| title_full | Association of CYP2D6*4 Polymorphism With Response to Atorvastatin in Patients With High Low-Density Lipoprotein Level in Northern Iran, Guilan Provice |
| title_fullStr | Association of CYP2D6*4 Polymorphism With Response to Atorvastatin in Patients With High Low-Density Lipoprotein Level in Northern Iran, Guilan Provice |
| title_full_unstemmed | Association of CYP2D6*4 Polymorphism With Response to Atorvastatin in Patients With High Low-Density Lipoprotein Level in Northern Iran, Guilan Provice |
| title_short | Association of CYP2D6*4 Polymorphism With Response to Atorvastatin in Patients With High Low-Density Lipoprotein Level in Northern Iran, Guilan Provice |
| title_sort | association of cyp2d6 4 polymorphism with response to atorvastatin in patients with high low density lipoprotein level in northern iran guilan provice |
| topic | atorvastatin cyp2d6 polymorphism ldl |
| url | http://journal.gums.ac.ir/article-1-2552-en.pdf |
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