Global spread and antimicrobial resistance of Aeromonas hydrophila in aquatic food animals: a systematic review and meta-analysis

Abstract Aeromonas hydrophila is a common zoonotic agent in aquatic environments that causes gastroenteritis and wound infections in both humans and animals through foodborne and hospital-acquired infection. Antimicrobial resistance (AMR) combination with virulence factors enhances treatment challen...

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Main Authors: Saharuetai Jeamsripong, Justice Opare Odoi, Manoj Kumar Shahi, Saran Anuntawirun, Nawaphorn Roongrojmongkhon, Anyarat Thiptara
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-14498-8
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author Saharuetai Jeamsripong
Justice Opare Odoi
Manoj Kumar Shahi
Saran Anuntawirun
Nawaphorn Roongrojmongkhon
Anyarat Thiptara
author_facet Saharuetai Jeamsripong
Justice Opare Odoi
Manoj Kumar Shahi
Saran Anuntawirun
Nawaphorn Roongrojmongkhon
Anyarat Thiptara
author_sort Saharuetai Jeamsripong
collection DOAJ
description Abstract Aeromonas hydrophila is a common zoonotic agent in aquatic environments that causes gastroenteritis and wound infections in both humans and animals through foodborne and hospital-acquired infection. Antimicrobial resistance (AMR) combination with virulence factors enhances treatment challenging. The prevalence and AMR of Aeromonas hydrophila have been increasingly reported, posing a significant threat to both animal and public health. This systematic review and meta-analysis aimed to determine the prevalence of A. hydrophila and its resistance to aquatic food animals. A comprehensive search for relevant studies was conducted on Google Scholar, PubMed, ScienceDirect, and Scopus, following the PRISMA guidelines, covering studies from January 2020 to December 2024. The quality of the included publications was evaluated using the Joanna Briggs Institute critical appraisal tool. Differences in the prevalence and AMR of A. hydrophila were assessed using a random-effect model. A total of 14,077 studies were screened, and 14 publications were included. Bacterial isolation of A. hydrophila was achieved using various standard protocols, involving culture on Rimler-Shotts (RS) agar and Tryptic Soy Agar (TSA), with or without antimicrobials. Subsequent biochemical identification confirmed the isolates. The pool prevalence of A. hydrophila (30.7%, 95% C.I.:17.0-46.3%), and the distribution of virulence genes were 71.2%. The most common resistance observed to penicillin (80.7%), oxytetracycline (69.9%) and macrolides (67.8%). The most prevalent AMR genes identified were bla TEM (67.0%), followed by tetA (63.7%). The increasing presence of A. hydrophila in aquaculture suggests a considerable risk of disease. The prevalence of both A. hydrophila and AMR was higher in Africa than in Asia, indicating regional variations in the AMR pattern. However, monitoring and surveillance of A. hydrophila remained limited. A major limitation of this study was the heterogeneity in effect estimates across the selected studies. Nonetheless, the quality assessment conducted indicated that this variability did not compromise the consistency or reliability of the findings.
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spelling doaj-art-9d3dd781590b40ba96cca77c8b57bce12025-08-20T03:05:22ZengNature PortfolioScientific Reports2045-23222025-08-0115111510.1038/s41598-025-14498-8Global spread and antimicrobial resistance of Aeromonas hydrophila in aquatic food animals: a systematic review and meta-analysisSaharuetai Jeamsripong0Justice Opare Odoi1Manoj Kumar Shahi2Saran Anuntawirun3Nawaphorn Roongrojmongkhon4Anyarat Thiptara5Research Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityVeterinary Research and Development Center (Upper Southern Region)Abstract Aeromonas hydrophila is a common zoonotic agent in aquatic environments that causes gastroenteritis and wound infections in both humans and animals through foodborne and hospital-acquired infection. Antimicrobial resistance (AMR) combination with virulence factors enhances treatment challenging. The prevalence and AMR of Aeromonas hydrophila have been increasingly reported, posing a significant threat to both animal and public health. This systematic review and meta-analysis aimed to determine the prevalence of A. hydrophila and its resistance to aquatic food animals. A comprehensive search for relevant studies was conducted on Google Scholar, PubMed, ScienceDirect, and Scopus, following the PRISMA guidelines, covering studies from January 2020 to December 2024. The quality of the included publications was evaluated using the Joanna Briggs Institute critical appraisal tool. Differences in the prevalence and AMR of A. hydrophila were assessed using a random-effect model. A total of 14,077 studies were screened, and 14 publications were included. Bacterial isolation of A. hydrophila was achieved using various standard protocols, involving culture on Rimler-Shotts (RS) agar and Tryptic Soy Agar (TSA), with or without antimicrobials. Subsequent biochemical identification confirmed the isolates. The pool prevalence of A. hydrophila (30.7%, 95% C.I.:17.0-46.3%), and the distribution of virulence genes were 71.2%. The most common resistance observed to penicillin (80.7%), oxytetracycline (69.9%) and macrolides (67.8%). The most prevalent AMR genes identified were bla TEM (67.0%), followed by tetA (63.7%). The increasing presence of A. hydrophila in aquaculture suggests a considerable risk of disease. The prevalence of both A. hydrophila and AMR was higher in Africa than in Asia, indicating regional variations in the AMR pattern. However, monitoring and surveillance of A. hydrophila remained limited. A major limitation of this study was the heterogeneity in effect estimates across the selected studies. Nonetheless, the quality assessment conducted indicated that this variability did not compromise the consistency or reliability of the findings.https://doi.org/10.1038/s41598-025-14498-8Aeromonas hydrophillaAntimicrobial resistanceESBLMultidrug resistanceVirulence factors
spellingShingle Saharuetai Jeamsripong
Justice Opare Odoi
Manoj Kumar Shahi
Saran Anuntawirun
Nawaphorn Roongrojmongkhon
Anyarat Thiptara
Global spread and antimicrobial resistance of Aeromonas hydrophila in aquatic food animals: a systematic review and meta-analysis
Scientific Reports
Aeromonas hydrophilla
Antimicrobial resistance
ESBL
Multidrug resistance
Virulence factors
title Global spread and antimicrobial resistance of Aeromonas hydrophila in aquatic food animals: a systematic review and meta-analysis
title_full Global spread and antimicrobial resistance of Aeromonas hydrophila in aquatic food animals: a systematic review and meta-analysis
title_fullStr Global spread and antimicrobial resistance of Aeromonas hydrophila in aquatic food animals: a systematic review and meta-analysis
title_full_unstemmed Global spread and antimicrobial resistance of Aeromonas hydrophila in aquatic food animals: a systematic review and meta-analysis
title_short Global spread and antimicrobial resistance of Aeromonas hydrophila in aquatic food animals: a systematic review and meta-analysis
title_sort global spread and antimicrobial resistance of aeromonas hydrophila in aquatic food animals a systematic review and meta analysis
topic Aeromonas hydrophilla
Antimicrobial resistance
ESBL
Multidrug resistance
Virulence factors
url https://doi.org/10.1038/s41598-025-14498-8
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