Molecular and Biological Characterization of an Isolate of Fusarium graminearum dsRNA mycovirus 4 (FgV4) from a New Host <i>Fusarium pseudograminearum</i>
Wheat Fusarium crown rot (FCR), mainly caused by <i>Fusarium pseudograminearum</i>, is one of the most important diseases. Some mycoviruses are reported to have a hypovirulence trait and considered as a biocontrol agent for plant fungal diseases. In most cases, mycovirus biological effec...
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2025-02-01
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| author | Guoping Ma Yueli Zhang Liguo Ma Kai Cui Bo Zhang Hang Jiang Kai Qi Junshan Qi |
| author_facet | Guoping Ma Yueli Zhang Liguo Ma Kai Cui Bo Zhang Hang Jiang Kai Qi Junshan Qi |
| author_sort | Guoping Ma |
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| description | Wheat Fusarium crown rot (FCR), mainly caused by <i>Fusarium pseudograminearum</i>, is one of the most important diseases. Some mycoviruses are reported to have a hypovirulence trait and considered as a biocontrol agent for plant fungal diseases. In most cases, mycovirus biological effects have not been explored clearly. In this study, we identified and characterized a novel isolate of double-stranded RNA (dsRNA) mycovirus, Fusarium graminearum dsRNA mycovirus 4 (FgV4), from a new host, an isolate WC9-2 of <i>F. pseudograminearum</i>. The genome of FgV4-WC9-2 includes two dsRNA segments of 2194 bp and 1738 bp. FgV4-WC9-2 dsRNA1 contains a single open reading frame (ORF1), which encodes a protein of 675 amino acids (aa) and has a conserved RNA-dependent RNA polymerase (RdRp) domain. FgV4-WC9-2 dsRNA2 contains two discontinuous ORFs (ORF2-1 and ORF2-2) that code for hypothetical proteins with unknown function. Biological characteristics research has shown that FgV4-WC9-2 infection did not change the colony morphology, but it could significantly decrease colony growth rate. FgV4-WC9-2 could also reduce the sporulation ability, change the conidia size and reduce the pathogenicity of the host to a certain extent. This study is the first to describe a hypovirulence-associated orthocurvulavirus infecting <i>F. pseudograminearum</i>, which has the potential to assist with FCR disease biological management. |
| format | Article |
| id | doaj-art-9d3a24e8beba42abad4cd2eb41d66061 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-9d3a24e8beba42abad4cd2eb41d660612025-08-20T03:11:21ZengMDPI AGMicroorganisms2076-26072025-02-0113241810.3390/microorganisms13020418Molecular and Biological Characterization of an Isolate of Fusarium graminearum dsRNA mycovirus 4 (FgV4) from a New Host <i>Fusarium pseudograminearum</i>Guoping Ma0Yueli Zhang1Liguo Ma2Kai Cui3Bo Zhang4Hang Jiang5Kai Qi6Junshan Qi7Shandong Key Laboratory for Green Prevention and Control of Agricultural Pests, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaShandong Key Laboratory for Green Prevention and Control of Agricultural Pests, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaShandong Key Laboratory for Green Prevention and Control of Agricultural Pests, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaShandong Provincial Key Laboratory of Test Technology on Food Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaShandong Key Laboratory for Green Prevention and Control of Agricultural Pests, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaShandong Key Laboratory for Green Prevention and Control of Agricultural Pests, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaShandong Key Laboratory for Green Prevention and Control of Agricultural Pests, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaShandong Key Laboratory for Green Prevention and Control of Agricultural Pests, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaWheat Fusarium crown rot (FCR), mainly caused by <i>Fusarium pseudograminearum</i>, is one of the most important diseases. Some mycoviruses are reported to have a hypovirulence trait and considered as a biocontrol agent for plant fungal diseases. In most cases, mycovirus biological effects have not been explored clearly. In this study, we identified and characterized a novel isolate of double-stranded RNA (dsRNA) mycovirus, Fusarium graminearum dsRNA mycovirus 4 (FgV4), from a new host, an isolate WC9-2 of <i>F. pseudograminearum</i>. The genome of FgV4-WC9-2 includes two dsRNA segments of 2194 bp and 1738 bp. FgV4-WC9-2 dsRNA1 contains a single open reading frame (ORF1), which encodes a protein of 675 amino acids (aa) and has a conserved RNA-dependent RNA polymerase (RdRp) domain. FgV4-WC9-2 dsRNA2 contains two discontinuous ORFs (ORF2-1 and ORF2-2) that code for hypothetical proteins with unknown function. Biological characteristics research has shown that FgV4-WC9-2 infection did not change the colony morphology, but it could significantly decrease colony growth rate. FgV4-WC9-2 could also reduce the sporulation ability, change the conidia size and reduce the pathogenicity of the host to a certain extent. This study is the first to describe a hypovirulence-associated orthocurvulavirus infecting <i>F. pseudograminearum</i>, which has the potential to assist with FCR disease biological management.https://www.mdpi.com/2076-2607/13/2/418<i>Fusarium pseudograminearum</i>mycovirusorthocurvulavirusbiological characteristicsbiocontrol |
| spellingShingle | Guoping Ma Yueli Zhang Liguo Ma Kai Cui Bo Zhang Hang Jiang Kai Qi Junshan Qi Molecular and Biological Characterization of an Isolate of Fusarium graminearum dsRNA mycovirus 4 (FgV4) from a New Host <i>Fusarium pseudograminearum</i> Microorganisms <i>Fusarium pseudograminearum</i> mycovirus orthocurvulavirus biological characteristics biocontrol |
| title | Molecular and Biological Characterization of an Isolate of Fusarium graminearum dsRNA mycovirus 4 (FgV4) from a New Host <i>Fusarium pseudograminearum</i> |
| title_full | Molecular and Biological Characterization of an Isolate of Fusarium graminearum dsRNA mycovirus 4 (FgV4) from a New Host <i>Fusarium pseudograminearum</i> |
| title_fullStr | Molecular and Biological Characterization of an Isolate of Fusarium graminearum dsRNA mycovirus 4 (FgV4) from a New Host <i>Fusarium pseudograminearum</i> |
| title_full_unstemmed | Molecular and Biological Characterization of an Isolate of Fusarium graminearum dsRNA mycovirus 4 (FgV4) from a New Host <i>Fusarium pseudograminearum</i> |
| title_short | Molecular and Biological Characterization of an Isolate of Fusarium graminearum dsRNA mycovirus 4 (FgV4) from a New Host <i>Fusarium pseudograminearum</i> |
| title_sort | molecular and biological characterization of an isolate of fusarium graminearum dsrna mycovirus 4 fgv4 from a new host i fusarium pseudograminearum i |
| topic | <i>Fusarium pseudograminearum</i> mycovirus orthocurvulavirus biological characteristics biocontrol |
| url | https://www.mdpi.com/2076-2607/13/2/418 |
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