Aluminum-induced changes in cell-wall pectin methylesterase activity of wheat seedlings in relation to their aluminum tolerance
The effects of aluminum (Al) on root elongation and changes in cell wall pectin methylesterase (PME) activity in the roots of Al-resistant (Xiaimai-1) and Al-sensitive (Fu-84) genotypes of wheat (Triticum aestivum L.) were examined. Root elongation was inhibited in both genotypes when wheat roots we...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2006-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2006.02.0145 |
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| Summary: | The effects of aluminum (Al) on root elongation and changes in cell wall pectin methylesterase (PME) activity in the roots of Al-resistant (Xiaimai-1) and Al-sensitive (Fu-84) genotypes of wheat (Triticum aestivum L.) were examined. Root elongation was inhibited in both genotypes when wheat roots were exposed to 30 µmol·L<sup>-1</sup> AICI<sub>3</sub> solution for 6 h or 24 h, the inhibition of root elongation being more prominent in the Al-sensitive genotype (Fu-84). Al content was significantly higher in 0-10 mm root segments than that in 10-20 mm root segments; and more Al accumulated in Fu-84 than in Xiaimai-1 in 0-10 mm root segments. Pectin uronic acid content in Fu-84 was higher than in Xiaimai-1 in 0-10 mm root segments, which was increased under Al stressed condition, more conspicuously in the Al-sensitive genotype Fu-84. The PME activity in roots of Xiaimai-1 was less affected by Al treatment, the PME activity in 0-10 mm root segment of Fu-84 was increased when the duration was 6h and was decreased when the duration was 24h. The root tip (0-10 mm root segment) cell wall PME activity in Fu-84 was enhanced after 6h Al treatment but decreased after 24 h Al treatment. Whether the plant was treated by Al or not, the Al-sensitive genotype Fu-84 always showed a higher cell wall PME activity than did the Al-resistant genotype Xiaimai-1 in 0-10 mm root segment. The Al-sensitive genotype Fu-84 always showed a higher PME activity and the activity of PME and pectin content increased after short-term exposure to Al, which results in more Al binding sites in Fu-84 than in Xiaimai-1. The present data emphasize the importance of the cell wall PME activity for the manifestation of Al toxicity and resistance. |
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| ISSN: | 1008-9209 2097-5155 |