Evaluation of the effects of clearing agents, fixation, and process durations on cardiovascular tissue imaging with second harmonic generation and multi-photon modalities

IntroductionProtocols for tissue clearing have been established and optimized for the central nervous system. However, significant modifications are required for clearing different tissue types. Therefore, effective optical clearing for cardiovascular tissue remains a major challenge. The goal of th...

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Main Authors: Maedeh Makki, Zachary A. Molander, Sergio A. Pineda-Castillo, Devin W. Laurence, Shubhra Singhal, Yasmin Eltwafsha, Gerhard A. Holzapfel, Tingting Gu, Chung-Hao Lee
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Bioengineering and Biotechnology
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Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2025.1606425/full
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