Simultaneous electrochemical determination of Staphylococcus aureus and Listeria monocytogenes based on Ti3C2Tx MXene nanoribbons/copper nanoparticles coupled with aptamer-gated probe/UiO-66 framework
Foodborne pathogenic bacteria pose severe threats to human health and often coexist within the same environment. Thus, developing a sensitive and reliable method for their simultaneous detection is critically important. In this study, we first synthesized Ti3C2Tx MXene nanoribbons/copper nanoparticl...
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Elsevier
2025-06-01
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| Series: | Sensing and Bio-Sensing Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180425000765 |
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| author | Xiaohua Jiang Zhiwen Lv Changquan Rao Xiaowen Chen |
| author_facet | Xiaohua Jiang Zhiwen Lv Changquan Rao Xiaowen Chen |
| author_sort | Xiaohua Jiang |
| collection | DOAJ |
| description | Foodborne pathogenic bacteria pose severe threats to human health and often coexist within the same environment. Thus, developing a sensitive and reliable method for their simultaneous detection is critically important. In this study, we first synthesized Ti3C2Tx MXene nanoribbons/copper nanoparticles (Ti3C2TxNR/Cu) via a simple self-reduction method to modify electrodes for signal amplification. Concurrently, we constructed UiO-66 MOFs encapsulated with distinct probes and gated by target-specific aptamers (Apts) as bio-gatekeepers. We then proposed an innovative electrochemical strategy for the simultaneous detection of two model pathogens: Staphylococcus aureus (S. aureus) and Listeria monocytogenes (LM). The Ti3C2TxNR/Cu nanohybrid synergistically enhanced the current signals of the probes by leveraging the combined advantages of Ti3C2TxNR and Cu nanoparticles. For S. aureus detection, methylene blue (MB) and S. aureus-specific aptamer (S-Apt) were employed as the probe and bio-gatekeeper, respectively, while ferrocene (Fc) and LM-specific aptamer (L-Apt) were used for LM. Upon target binding, the Apts dissociated from the UiO-66 MOFs, releasing the encapsulated probes (MB or Fc). The resulting current signals, measured at the Ti3C2TxNR/Cu electrode, enabled the quantitative and simultaneous detection of both pathogens. This platform's modular design allows easy adaptation for detecting other bacteria by simply replacing the aptamers, offering broad potential applications in controlling food safety. |
| format | Article |
| id | doaj-art-9d2b897b610b46368bb77f99584249b8 |
| institution | DOAJ |
| issn | 2214-1804 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj-art-9d2b897b610b46368bb77f99584249b82025-08-20T02:39:33ZengElsevierSensing and Bio-Sensing Research2214-18042025-06-014810081010.1016/j.sbsr.2025.100810Simultaneous electrochemical determination of Staphylococcus aureus and Listeria monocytogenes based on Ti3C2Tx MXene nanoribbons/copper nanoparticles coupled with aptamer-gated probe/UiO-66 frameworkXiaohua Jiang0Zhiwen Lv1Changquan Rao2Xiaowen Chen3School of Undergraduate Education, Shenzhen Polytechnic University, Shenzhen 518055, PR China; Corresponding authors.School of Materials & Enviromental Engineering, Shenzhen Polytechnic University, Shenzhen 518055, PR ChinaSchool of Materials & Enviromental Engineering, Shenzhen Polytechnic University, Shenzhen 518055, PR ChinaSchool of Materials & Enviromental Engineering, Shenzhen Polytechnic University, Shenzhen 518055, PR China; Corresponding authors.Foodborne pathogenic bacteria pose severe threats to human health and often coexist within the same environment. Thus, developing a sensitive and reliable method for their simultaneous detection is critically important. In this study, we first synthesized Ti3C2Tx MXene nanoribbons/copper nanoparticles (Ti3C2TxNR/Cu) via a simple self-reduction method to modify electrodes for signal amplification. Concurrently, we constructed UiO-66 MOFs encapsulated with distinct probes and gated by target-specific aptamers (Apts) as bio-gatekeepers. We then proposed an innovative electrochemical strategy for the simultaneous detection of two model pathogens: Staphylococcus aureus (S. aureus) and Listeria monocytogenes (LM). The Ti3C2TxNR/Cu nanohybrid synergistically enhanced the current signals of the probes by leveraging the combined advantages of Ti3C2TxNR and Cu nanoparticles. For S. aureus detection, methylene blue (MB) and S. aureus-specific aptamer (S-Apt) were employed as the probe and bio-gatekeeper, respectively, while ferrocene (Fc) and LM-specific aptamer (L-Apt) were used for LM. Upon target binding, the Apts dissociated from the UiO-66 MOFs, releasing the encapsulated probes (MB or Fc). The resulting current signals, measured at the Ti3C2TxNR/Cu electrode, enabled the quantitative and simultaneous detection of both pathogens. This platform's modular design allows easy adaptation for detecting other bacteria by simply replacing the aptamers, offering broad potential applications in controlling food safety.http://www.sciencedirect.com/science/article/pii/S2214180425000765PathogensElectrochemical sensorSimultaneous detectionMXeneProbeMOFs |
| spellingShingle | Xiaohua Jiang Zhiwen Lv Changquan Rao Xiaowen Chen Simultaneous electrochemical determination of Staphylococcus aureus and Listeria monocytogenes based on Ti3C2Tx MXene nanoribbons/copper nanoparticles coupled with aptamer-gated probe/UiO-66 framework Sensing and Bio-Sensing Research Pathogens Electrochemical sensor Simultaneous detection MXene Probe MOFs |
| title | Simultaneous electrochemical determination of Staphylococcus aureus and Listeria monocytogenes based on Ti3C2Tx MXene nanoribbons/copper nanoparticles coupled with aptamer-gated probe/UiO-66 framework |
| title_full | Simultaneous electrochemical determination of Staphylococcus aureus and Listeria monocytogenes based on Ti3C2Tx MXene nanoribbons/copper nanoparticles coupled with aptamer-gated probe/UiO-66 framework |
| title_fullStr | Simultaneous electrochemical determination of Staphylococcus aureus and Listeria monocytogenes based on Ti3C2Tx MXene nanoribbons/copper nanoparticles coupled with aptamer-gated probe/UiO-66 framework |
| title_full_unstemmed | Simultaneous electrochemical determination of Staphylococcus aureus and Listeria monocytogenes based on Ti3C2Tx MXene nanoribbons/copper nanoparticles coupled with aptamer-gated probe/UiO-66 framework |
| title_short | Simultaneous electrochemical determination of Staphylococcus aureus and Listeria monocytogenes based on Ti3C2Tx MXene nanoribbons/copper nanoparticles coupled with aptamer-gated probe/UiO-66 framework |
| title_sort | simultaneous electrochemical determination of staphylococcus aureus and listeria monocytogenes based on ti3c2tx mxene nanoribbons copper nanoparticles coupled with aptamer gated probe uio 66 framework |
| topic | Pathogens Electrochemical sensor Simultaneous detection MXene Probe MOFs |
| url | http://www.sciencedirect.com/science/article/pii/S2214180425000765 |
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