The Significance of Serum and Fecal Levels of Interleukin-6 and Interleukin-8 in Hospitalized Children with Acute Rotavirus and Norovirus Gastroenteritis

Rotavirus and norovirus are the most common known causes of viral gastroenteritis in children. This study examined the association between serum interleukin 6 (IL-6) and interleukin 8 (IL-8) levels and disease severity in the acute phase of rotavirus and norovirus gastroenteritis in children, and it...

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Main Authors: Shan-Ming Chen, Ching-Pin Lin, Jeng-Dau Tsai, Yu-Hua Chao, Ji-Nan Sheu
Format: Article
Language:English
Published: Elsevier 2014-04-01
Series:Pediatrics and Neonatology
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Online Access:http://www.sciencedirect.com/science/article/pii/S1875957213001101
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author Shan-Ming Chen
Ching-Pin Lin
Jeng-Dau Tsai
Yu-Hua Chao
Ji-Nan Sheu
author_facet Shan-Ming Chen
Ching-Pin Lin
Jeng-Dau Tsai
Yu-Hua Chao
Ji-Nan Sheu
author_sort Shan-Ming Chen
collection DOAJ
description Rotavirus and norovirus are the most common known causes of viral gastroenteritis in children. This study examined the association between serum interleukin 6 (IL-6) and interleukin 8 (IL-8) levels and disease severity in the acute phase of rotavirus and norovirus gastroenteritis in children, and it also explored the role of fecal cytokine levels in children with viral and bacterial gastroenteritis. Methods: This prospective study enrolled patients aged 4 months to 14 years admitted with acute gastroenteritis in a tertiary care center. Peripheral blood samples were collected for IL-6 and IL-8 assays within the first 3 days of diarrhea. Stool samples were obtained from the patients in the first 24 hours after admission. Results: Serum IL-6 and IL-8 were measured in children with viral (n = 66) and bacterial (n = 23) infections, and in healthy controls (n = 10). In the acute phase of gastroenteritis, a moderately positive correlation was found between serum IL-6 levels and disease severity (rs = 0.41, p < 0.01). Serum IL-8 levels correlated with the duration of fever (rs = 0.28, p = 0.03). Fecal IL-6 levels correlated with the maximum number of daily bowel movements (rs = 0.35, p < 0.05). Rotavirus infection induced significantly higher serum IL-8 levels than norovirus infection (p < 0.05). Receiver operating characteristic (ROC) curve analysis showed that absolute neutrophil count (ANC), maximum body temperature (BT), and Vesikari score were significant predictors in discriminating rotavirus from norovirus gastroenteritis. Conclusion: IL-6 and IL-8 are involved in the pathogenesis of acute gastroenteritis in both rotavirus and norovirus. An ANC of less than 9000/mm3, maximum BT of less than 38.2°C, and Vesikari score of less than 14 at the end of the course are potential predictors of norovirus infection in children compared with rotavirus gastroenteritis.
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spelling doaj-art-9d2a98da18e9480095866ae01ca495512025-08-20T02:13:39ZengElsevierPediatrics and Neonatology1875-95722014-04-0155212012610.1016/j.pedneo.2013.05.008The Significance of Serum and Fecal Levels of Interleukin-6 and Interleukin-8 in Hospitalized Children with Acute Rotavirus and Norovirus GastroenteritisShan-Ming Chen0Ching-Pin Lin1Jeng-Dau Tsai2Yu-Hua Chao3Ji-Nan Sheu4Institute of Medicine, Chung Shan Medical University, Taichung, TaiwanDepartment of Internal Medicine, Chung Shan Medical University Hospital, Taichung, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung, TaiwanDepartment of Pediatrics, Chung Shan Medical University Hospital, Taichung, TaiwanRotavirus and norovirus are the most common known causes of viral gastroenteritis in children. This study examined the association between serum interleukin 6 (IL-6) and interleukin 8 (IL-8) levels and disease severity in the acute phase of rotavirus and norovirus gastroenteritis in children, and it also explored the role of fecal cytokine levels in children with viral and bacterial gastroenteritis. Methods: This prospective study enrolled patients aged 4 months to 14 years admitted with acute gastroenteritis in a tertiary care center. Peripheral blood samples were collected for IL-6 and IL-8 assays within the first 3 days of diarrhea. Stool samples were obtained from the patients in the first 24 hours after admission. Results: Serum IL-6 and IL-8 were measured in children with viral (n = 66) and bacterial (n = 23) infections, and in healthy controls (n = 10). In the acute phase of gastroenteritis, a moderately positive correlation was found between serum IL-6 levels and disease severity (rs = 0.41, p < 0.01). Serum IL-8 levels correlated with the duration of fever (rs = 0.28, p = 0.03). Fecal IL-6 levels correlated with the maximum number of daily bowel movements (rs = 0.35, p < 0.05). Rotavirus infection induced significantly higher serum IL-8 levels than norovirus infection (p < 0.05). Receiver operating characteristic (ROC) curve analysis showed that absolute neutrophil count (ANC), maximum body temperature (BT), and Vesikari score were significant predictors in discriminating rotavirus from norovirus gastroenteritis. Conclusion: IL-6 and IL-8 are involved in the pathogenesis of acute gastroenteritis in both rotavirus and norovirus. An ANC of less than 9000/mm3, maximum BT of less than 38.2°C, and Vesikari score of less than 14 at the end of the course are potential predictors of norovirus infection in children compared with rotavirus gastroenteritis.http://www.sciencedirect.com/science/article/pii/S1875957213001101interleukin-6interleukin-8norovirusrotavirus
spellingShingle Shan-Ming Chen
Ching-Pin Lin
Jeng-Dau Tsai
Yu-Hua Chao
Ji-Nan Sheu
The Significance of Serum and Fecal Levels of Interleukin-6 and Interleukin-8 in Hospitalized Children with Acute Rotavirus and Norovirus Gastroenteritis
Pediatrics and Neonatology
interleukin-6
interleukin-8
norovirus
rotavirus
title The Significance of Serum and Fecal Levels of Interleukin-6 and Interleukin-8 in Hospitalized Children with Acute Rotavirus and Norovirus Gastroenteritis
title_full The Significance of Serum and Fecal Levels of Interleukin-6 and Interleukin-8 in Hospitalized Children with Acute Rotavirus and Norovirus Gastroenteritis
title_fullStr The Significance of Serum and Fecal Levels of Interleukin-6 and Interleukin-8 in Hospitalized Children with Acute Rotavirus and Norovirus Gastroenteritis
title_full_unstemmed The Significance of Serum and Fecal Levels of Interleukin-6 and Interleukin-8 in Hospitalized Children with Acute Rotavirus and Norovirus Gastroenteritis
title_short The Significance of Serum and Fecal Levels of Interleukin-6 and Interleukin-8 in Hospitalized Children with Acute Rotavirus and Norovirus Gastroenteritis
title_sort significance of serum and fecal levels of interleukin 6 and interleukin 8 in hospitalized children with acute rotavirus and norovirus gastroenteritis
topic interleukin-6
interleukin-8
norovirus
rotavirus
url http://www.sciencedirect.com/science/article/pii/S1875957213001101
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