Clinical characteristics of preterm and term infants with Ureaplasma in gastric fluid

Background: Ureaplasma spp. is an endemic microorganism that causes placental chorioamnionitis or preterm delivery in pregnant women, and the occurrence of bronchopulmonary dysplasia or intraventricular hemorrhaging in preterm infants after birth, although the pathogenicity of Ureaplasma remains con...

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Main Authors: Yoshiichi Abe, Masanori Inoue, Kazuhito Sekiguchi, Satoko Nakano, Yasuhiro Tomaru, Tomoki Maeda, Norio Shimizu, Kenji Ihara
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:Pediatrics and Neonatology
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Online Access:http://www.sciencedirect.com/science/article/pii/S1875957223001493
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author Yoshiichi Abe
Masanori Inoue
Kazuhito Sekiguchi
Satoko Nakano
Yasuhiro Tomaru
Tomoki Maeda
Norio Shimizu
Kenji Ihara
author_facet Yoshiichi Abe
Masanori Inoue
Kazuhito Sekiguchi
Satoko Nakano
Yasuhiro Tomaru
Tomoki Maeda
Norio Shimizu
Kenji Ihara
author_sort Yoshiichi Abe
collection DOAJ
description Background: Ureaplasma spp. is an endemic microorganism that causes placental chorioamnionitis or preterm delivery in pregnant women, and the occurrence of bronchopulmonary dysplasia or intraventricular hemorrhaging in preterm infants after birth, although the pathogenicity of Ureaplasma remains controversial. The association between Ureaplasma exposure and the symptoms or outcomes of infected mothers or their infants born at term remains poorly understood. We investigated the clinical characteristics of preterm and term infants with or without Ureaplasma in their gastric fluid. Methods: Gastric fluid samples were collected from 47 newborns in the neonatal intensive-care unit immediately after birth and tested using multiplex polymerase chain reaction (PCR) assays targeting Ureaplasma spp., Ureaplasma parvum, and Ureaplasma urealyticum. The clinical findings and outcomes of the neonates and their mothers were retrospectively evaluated. Results: Ureaplasma spp. were detected in 9/47 samples (19%) by multiplex PCR assays. In all cases, the subspecies was U. parvum. The Ureaplasma-positive group had a significantly higher incidence of chorioamnionitis in utero than the Ureaplasma-negative group. Regarding preterm infants, the IgM levels in the Ureaplasma-positive group were significantly higher than in the Ureaplasma-negative group. In contrast, in term infants, the rates of a non-reassuring fetal status, a maternal fever, and maternal leukocyte counts and maternal C-reactive protein levels within five days before delivery in the Ureaplasma-positive group were significantly higher than those in the Ureaplasma-negative group. All three extremely-low-birth-weight infants with Ureaplasma developed bronchopulmonary dysplasia. The length of hospitalization in the Ureaplasma-positive group was almost same as that in the Ureaplasma-negative group for term infants. Conclusion: Mothers or their fetuses with exposure to Ureaplasma expressed characteristic clinical features during pregnancy and after birth.
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spelling doaj-art-9d2a02db0c0a4632bc623a7efe7c23352025-08-20T03:04:44ZengElsevierPediatrics and Neonatology1875-95722024-03-0165217017610.1016/j.pedneo.2023.04.016Clinical characteristics of preterm and term infants with Ureaplasma in gastric fluidYoshiichi Abe0Masanori Inoue1Kazuhito Sekiguchi2Satoko Nakano3Yasuhiro Tomaru4Tomoki Maeda5Norio Shimizu6Kenji Ihara7Department of Pediatrics, Faculty of Medicine, Oita University, Oita, Japan; Department of Pediatrics, Koseiren Tsurumi Hospital, Oita, JapanDepartment of Pediatrics, Faculty of Medicine, Oita University, Oita, Japan; Corresponding author. Department of Pediatrics, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama, Yufu, Oita 879-5593, Japan.Department of Pediatrics, Faculty of Medicine, Oita University, Oita, JapanDepartment of Ophthalmology, Faculty of Medicine, Oita University, Oita, JapanCenter for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Pediatrics, Faculty of Medicine, Oita University, Oita, JapanCenter for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Pediatrics, Faculty of Medicine, Oita University, Oita, JapanBackground: Ureaplasma spp. is an endemic microorganism that causes placental chorioamnionitis or preterm delivery in pregnant women, and the occurrence of bronchopulmonary dysplasia or intraventricular hemorrhaging in preterm infants after birth, although the pathogenicity of Ureaplasma remains controversial. The association between Ureaplasma exposure and the symptoms or outcomes of infected mothers or their infants born at term remains poorly understood. We investigated the clinical characteristics of preterm and term infants with or without Ureaplasma in their gastric fluid. Methods: Gastric fluid samples were collected from 47 newborns in the neonatal intensive-care unit immediately after birth and tested using multiplex polymerase chain reaction (PCR) assays targeting Ureaplasma spp., Ureaplasma parvum, and Ureaplasma urealyticum. The clinical findings and outcomes of the neonates and their mothers were retrospectively evaluated. Results: Ureaplasma spp. were detected in 9/47 samples (19%) by multiplex PCR assays. In all cases, the subspecies was U. parvum. The Ureaplasma-positive group had a significantly higher incidence of chorioamnionitis in utero than the Ureaplasma-negative group. Regarding preterm infants, the IgM levels in the Ureaplasma-positive group were significantly higher than in the Ureaplasma-negative group. In contrast, in term infants, the rates of a non-reassuring fetal status, a maternal fever, and maternal leukocyte counts and maternal C-reactive protein levels within five days before delivery in the Ureaplasma-positive group were significantly higher than those in the Ureaplasma-negative group. All three extremely-low-birth-weight infants with Ureaplasma developed bronchopulmonary dysplasia. The length of hospitalization in the Ureaplasma-positive group was almost same as that in the Ureaplasma-negative group for term infants. Conclusion: Mothers or their fetuses with exposure to Ureaplasma expressed characteristic clinical features during pregnancy and after birth.http://www.sciencedirect.com/science/article/pii/S1875957223001493gastric fluidmultiplex real-time PCRneonateUreaplasma
spellingShingle Yoshiichi Abe
Masanori Inoue
Kazuhito Sekiguchi
Satoko Nakano
Yasuhiro Tomaru
Tomoki Maeda
Norio Shimizu
Kenji Ihara
Clinical characteristics of preterm and term infants with Ureaplasma in gastric fluid
Pediatrics and Neonatology
gastric fluid
multiplex real-time PCR
neonate
Ureaplasma
title Clinical characteristics of preterm and term infants with Ureaplasma in gastric fluid
title_full Clinical characteristics of preterm and term infants with Ureaplasma in gastric fluid
title_fullStr Clinical characteristics of preterm and term infants with Ureaplasma in gastric fluid
title_full_unstemmed Clinical characteristics of preterm and term infants with Ureaplasma in gastric fluid
title_short Clinical characteristics of preterm and term infants with Ureaplasma in gastric fluid
title_sort clinical characteristics of preterm and term infants with ureaplasma in gastric fluid
topic gastric fluid
multiplex real-time PCR
neonate
Ureaplasma
url http://www.sciencedirect.com/science/article/pii/S1875957223001493
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