Protective Effects of HBSP on Ischemia Reperfusion and Cyclosporine A Induced Renal Injury
Ischemia reperfusion (IR) and cyclosporine A (CsA) injuries are unavoidable in kidney transplantation and are associated with allograft dysfunction. Herein, the effect and mechanism of a novel tissue protective peptide, helix B surface peptide (HBSP) derived from erythropoietin, were investigated in...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/758159 |
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| author | Yuanyuan Wu Junlin Zhang Feng Liu Cheng Yang Yufang Zhang Aifen Liu Lan Shi Yajun Wu Tongyu Zhu Michael L. Nicholson Yaping Fan Bin Yang |
| author_facet | Yuanyuan Wu Junlin Zhang Feng Liu Cheng Yang Yufang Zhang Aifen Liu Lan Shi Yajun Wu Tongyu Zhu Michael L. Nicholson Yaping Fan Bin Yang |
| author_sort | Yuanyuan Wu |
| collection | DOAJ |
| description | Ischemia reperfusion (IR) and cyclosporine A (CsA) injuries are unavoidable in kidney transplantation and are associated with allograft dysfunction. Herein, the effect and mechanism of a novel tissue protective peptide, helix B surface peptide (HBSP) derived from erythropoietin, were investigated in a rat model. The right kidney was subjected to 45 min ischemia, followed by left nephrectomy and 2-week reperfusion, with or without daily treatment of CsA 25 mg/kg and/or HBSP 8 nmol/kg. Blood urea nitrogen was increased by CsA but decreased by HBSP at 1 week and 2 weeks, while the same changes were revealed in urinary protein/creatinine only at 2 weeks. HBSP also significantly ameliorated tubulointerstitial damage and interstitial fibrosis, which were gradually increased by IR and CsA. In addition, apoptotic cells, infiltrated inflammatory cells, and active caspase-3+ cells were greatly reduced by HBSP in the both IR and IR + CsA groups. The 17 kD active caspase-3 protein was decreased by HBSP in the IR and IR + CsA kidneys, with decreased mRNA only in the IR + CsA kidneys. Taken together, it has been demonstrated, for the first time, that HBSP effectively improved renal function and tissue damage caused by IR and/or CsA, which might be through reducing caspase-3 activation and synthesis, apoptosis, and inflammation. |
| format | Article |
| id | doaj-art-9d2529230e7240fda5a6bacafd1ebc44 |
| institution | OA Journals |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
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| series | Clinical and Developmental Immunology |
| spelling | doaj-art-9d2529230e7240fda5a6bacafd1ebc442025-08-20T02:20:19ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/758159758159Protective Effects of HBSP on Ischemia Reperfusion and Cyclosporine A Induced Renal InjuryYuanyuan Wu0Junlin Zhang1Feng Liu2Cheng Yang3Yufang Zhang4Aifen Liu5Lan Shi6Yajun Wu7Tongyu Zhu8Michael L. Nicholson9Yaping Fan10Bin Yang11Department of Pathology and Comparative Medicine Institute, University of Nantong, Nantong, Jiangsu 226001, ChinaDepartment of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of Urology, Zhongshan Hospital, Fudan University and Shanghai Key Laboratory of Organ Transplantation, Shanghai 200032, ChinaMedical Research Centre, University of Nantong, Nantong, Jiangsu 226001, ChinaMedical Research Centre, University of Nantong, Nantong, Jiangsu 226001, ChinaDepartment of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of Urology, Zhongshan Hospital, Fudan University and Shanghai Key Laboratory of Organ Transplantation, Shanghai 200032, ChinaTransplant Group, Department of Infection, Immunity and Inflammation, University of Leicester and Leicester General Hospital, University Hospitals of Leicester, Leicester LE5 4PW, UKDepartment of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaDepartment of Nephrology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, ChinaIschemia reperfusion (IR) and cyclosporine A (CsA) injuries are unavoidable in kidney transplantation and are associated with allograft dysfunction. Herein, the effect and mechanism of a novel tissue protective peptide, helix B surface peptide (HBSP) derived from erythropoietin, were investigated in a rat model. The right kidney was subjected to 45 min ischemia, followed by left nephrectomy and 2-week reperfusion, with or without daily treatment of CsA 25 mg/kg and/or HBSP 8 nmol/kg. Blood urea nitrogen was increased by CsA but decreased by HBSP at 1 week and 2 weeks, while the same changes were revealed in urinary protein/creatinine only at 2 weeks. HBSP also significantly ameliorated tubulointerstitial damage and interstitial fibrosis, which were gradually increased by IR and CsA. In addition, apoptotic cells, infiltrated inflammatory cells, and active caspase-3+ cells were greatly reduced by HBSP in the both IR and IR + CsA groups. The 17 kD active caspase-3 protein was decreased by HBSP in the IR and IR + CsA kidneys, with decreased mRNA only in the IR + CsA kidneys. Taken together, it has been demonstrated, for the first time, that HBSP effectively improved renal function and tissue damage caused by IR and/or CsA, which might be through reducing caspase-3 activation and synthesis, apoptosis, and inflammation.http://dx.doi.org/10.1155/2013/758159 |
| spellingShingle | Yuanyuan Wu Junlin Zhang Feng Liu Cheng Yang Yufang Zhang Aifen Liu Lan Shi Yajun Wu Tongyu Zhu Michael L. Nicholson Yaping Fan Bin Yang Protective Effects of HBSP on Ischemia Reperfusion and Cyclosporine A Induced Renal Injury Clinical and Developmental Immunology |
| title | Protective Effects of HBSP on Ischemia Reperfusion and Cyclosporine A Induced Renal Injury |
| title_full | Protective Effects of HBSP on Ischemia Reperfusion and Cyclosporine A Induced Renal Injury |
| title_fullStr | Protective Effects of HBSP on Ischemia Reperfusion and Cyclosporine A Induced Renal Injury |
| title_full_unstemmed | Protective Effects of HBSP on Ischemia Reperfusion and Cyclosporine A Induced Renal Injury |
| title_short | Protective Effects of HBSP on Ischemia Reperfusion and Cyclosporine A Induced Renal Injury |
| title_sort | protective effects of hbsp on ischemia reperfusion and cyclosporine a induced renal injury |
| url | http://dx.doi.org/10.1155/2013/758159 |
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