Molecular identification and infection pathology of Mycobacterium spp. in captive wild animals in Pakistan
Introduction: Animal tuberculosis is an infectious, chronic, granulomatous, and debilitating disease affecting animals as well as humans. However, in recent decades, there have been many endemic geographic localities where animal tuberculosis has been identified in wildlife reservoirs, limiting the...
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The Journal of Infection in Developing Countries
2023-08-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/17287 |
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| author | Komal Akhtar Ishtiaq Ahmed Muhammad Yasin Tipu Tahir Yaqub Muhammad Shahid Aziz ur Rehman Muhammad Zishan Ahmad Ammar Ghani Aman Ullah Khan Adeem Rehman Rafie Muhammad Kamran Rafique Muhammad Saadullah Muhammad Kashif Asim Aslam |
| author_facet | Komal Akhtar Ishtiaq Ahmed Muhammad Yasin Tipu Tahir Yaqub Muhammad Shahid Aziz ur Rehman Muhammad Zishan Ahmad Ammar Ghani Aman Ullah Khan Adeem Rehman Rafie Muhammad Kamran Rafique Muhammad Saadullah Muhammad Kashif Asim Aslam |
| author_sort | Komal Akhtar |
| collection | DOAJ |
| description |
Introduction: Animal tuberculosis is an infectious, chronic, granulomatous, and debilitating disease affecting animals as well as humans. However, in recent decades, there have been many endemic geographic localities where animal tuberculosis has been identified in wildlife reservoirs, limiting the eradication program in cattle. This study aimed to identify animal tuberculosis in captive zoo animals in Pakistan.
Methodology: In total, 185 morbid zoo animals were brought for postmortem examination at a veterinary postmortem facility. During the macroscopic examination, these animals were thoroughly examined for the presence of suggestive gross lesions of animal tuberculosis (granulomas/tubercles), and the pattern and distribution of these lesions in different organs. The Ziehl-Neelsen (ZN) staining was performed on smears prepared from granulomatous lesions of lung tissue followed by molecular identification of M. bovis and M. tuberculosis DNA using polymerase chain reaction (PCR).
Results: The postmortem examination revealed that 8.1% (15/185) of animals had gross tuberculosis lesions on the lungs and lymph nodes. The ZN staining of tissue smears showed 5.40% positivity while M. bovis and M. tuberculosis DNA was identified in 3.78 % and 1.1% of investigated animals, respectively.
Conclusions: The study showed that animal tuberculosis is prevalent among wildlife in Pakistan and it may pose serious public health concerns to the people visiting these zoos and wildlife parks.
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| format | Article |
| id | doaj-art-9d1a1e2e7a6e43308b2f1ce9a60d4992 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2023-08-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-9d1a1e2e7a6e43308b2f1ce9a60d49922025-08-20T02:27:14ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802023-08-01170810.3855/jidc.17287Molecular identification and infection pathology of Mycobacterium spp. in captive wild animals in PakistanKomal Akhtar0Ishtiaq Ahmed1Muhammad Yasin Tipu2Tahir Yaqub3Muhammad Shahid4Aziz ur Rehman5Muhammad Zishan Ahmad6Ammar Ghani7Aman Ullah Khan8Adeem Rehman Rafie9Muhammad Kamran Rafique10Muhammad Saadullah11Muhammad Kashif12Asim Aslam13Department of Pathology, University of Veterinary and Animal Sciences, Lahore, PakistanDepartment of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences, Lahore’ sub-campus CVAS, Jhang, PakistanDepartment of Pathology, University of Veterinary and Animal Sciences, Lahore, PakistanInstitute of Microbiology, University of Veterinary and Animal Sciences, Lahore, PakistanVeterinary Research Institute, Peshawar, KPK, PakistanDepartment of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences, Lahore’ sub-campus CVAS, Jhang, PakistanDepartment of Veterinary Pathology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University, Rawalpindi, PakistanDepartment of Pathology, University of Veterinary and Animal Sciences, Lahore, PakistanDepartment of Pathobiology (Microbiology Section), University of Veterinary and Animal Sciences, Lahore’ sub-campus CVAS, Jhang, PakistanDepartment of Pathology, University of Veterinary and Animal Sciences, Lahore, PakistanDepartment of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences, Lahore’ sub-campus CVAS, Jhang, PakistanDepartment of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences, Lahore’ sub-campus CVAS, Jhang, PakistanDepartment of Clinical Sciences (Clinical Medicine section), University of Veterinary and Animal Sciences, Lahore’ sub-campus CVAS, Jhang, PakistanDepartment of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan Introduction: Animal tuberculosis is an infectious, chronic, granulomatous, and debilitating disease affecting animals as well as humans. However, in recent decades, there have been many endemic geographic localities where animal tuberculosis has been identified in wildlife reservoirs, limiting the eradication program in cattle. This study aimed to identify animal tuberculosis in captive zoo animals in Pakistan. Methodology: In total, 185 morbid zoo animals were brought for postmortem examination at a veterinary postmortem facility. During the macroscopic examination, these animals were thoroughly examined for the presence of suggestive gross lesions of animal tuberculosis (granulomas/tubercles), and the pattern and distribution of these lesions in different organs. The Ziehl-Neelsen (ZN) staining was performed on smears prepared from granulomatous lesions of lung tissue followed by molecular identification of M. bovis and M. tuberculosis DNA using polymerase chain reaction (PCR). Results: The postmortem examination revealed that 8.1% (15/185) of animals had gross tuberculosis lesions on the lungs and lymph nodes. The ZN staining of tissue smears showed 5.40% positivity while M. bovis and M. tuberculosis DNA was identified in 3.78 % and 1.1% of investigated animals, respectively. Conclusions: The study showed that animal tuberculosis is prevalent among wildlife in Pakistan and it may pose serious public health concerns to the people visiting these zoos and wildlife parks. https://jidc.org/index.php/journal/article/view/17287TuberculosisM. bovisM. tuberculosiswildlifepathologyPCR |
| spellingShingle | Komal Akhtar Ishtiaq Ahmed Muhammad Yasin Tipu Tahir Yaqub Muhammad Shahid Aziz ur Rehman Muhammad Zishan Ahmad Ammar Ghani Aman Ullah Khan Adeem Rehman Rafie Muhammad Kamran Rafique Muhammad Saadullah Muhammad Kashif Asim Aslam Molecular identification and infection pathology of Mycobacterium spp. in captive wild animals in Pakistan Journal of Infection in Developing Countries Tuberculosis M. bovis M. tuberculosis wildlife pathology PCR |
| title | Molecular identification and infection pathology of Mycobacterium spp. in captive wild animals in Pakistan |
| title_full | Molecular identification and infection pathology of Mycobacterium spp. in captive wild animals in Pakistan |
| title_fullStr | Molecular identification and infection pathology of Mycobacterium spp. in captive wild animals in Pakistan |
| title_full_unstemmed | Molecular identification and infection pathology of Mycobacterium spp. in captive wild animals in Pakistan |
| title_short | Molecular identification and infection pathology of Mycobacterium spp. in captive wild animals in Pakistan |
| title_sort | molecular identification and infection pathology of mycobacterium spp in captive wild animals in pakistan |
| topic | Tuberculosis M. bovis M. tuberculosis wildlife pathology PCR |
| url | https://jidc.org/index.php/journal/article/view/17287 |
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