PHF6 interacts with the LMO2 complex in T-cell acute lymphoblastic leukemia
The transcriptional mediator LIM domain only 2 (LMO2) forms a large multi-protein complex together with TAL1/LYL1, HEB/E2A, LDB1 and GATA. This complex regulates transcription from the onset of hematopoietic development and during differentiation. Chromosomal rearrangements involving LMO2 and TAL1...
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| Format: | Article |
| Language: | English |
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Ferrata Storti Foundation
2025-07-01
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| Series: | Haematologica |
| Online Access: | https://haematologica.org/article/view/12167 |
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| _version_ | 1850117582048198656 |
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| author | Vesna S. Stanulović Sarah Binhassan Budoor A. Jaber Shimaa Alazmi Fatma M.B. Saleman Sandeep Potluri Guy Pratt Christian Ludwig Douglas G. Ward Maarten Hoogenkamp |
| author_facet | Vesna S. Stanulović Sarah Binhassan Budoor A. Jaber Shimaa Alazmi Fatma M.B. Saleman Sandeep Potluri Guy Pratt Christian Ludwig Douglas G. Ward Maarten Hoogenkamp |
| author_sort | Vesna S. Stanulović |
| collection | DOAJ |
| description |
The transcriptional mediator LIM domain only 2 (LMO2) forms a large multi-protein complex together with TAL1/LYL1, HEB/E2A, LDB1 and GATA. This complex regulates transcription from the onset of hematopoietic development and during differentiation. Chromosomal rearrangements involving LMO2 and TAL1 are causative for T-cell lymphoblastic leukemia (TALL). We have identified Plant Homeodomain (PHD)-like Finger 6 (PHF6) as a new LMO2 interacting factor. Somatic mutations in PHF6 have been found to occur in several types of leukemia. We show that PHF6 interacts with LMO2 as a part of the TAL1, GATA2, LDB1 complex in T-ALL and binds to the DNA. These findings show that PHF6 associates with the TAL1/LMO2/LDB1/GATA2 complex and regulates genes that have a major role in blood development, such as SPI1 (PU.1).
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| format | Article |
| id | doaj-art-9d06280a019f42a3a229e0362f96a5bf |
| institution | OA Journals |
| issn | 0390-6078 1592-8721 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Ferrata Storti Foundation |
| record_format | Article |
| series | Haematologica |
| spelling | doaj-art-9d06280a019f42a3a229e0362f96a5bf2025-08-20T02:36:03ZengFerrata Storti FoundationHaematologica0390-60781592-87212025-07-01999110.3324/haematol.2024.287124PHF6 interacts with the LMO2 complex in T-cell acute lymphoblastic leukemiaVesna S. Stanulović0Sarah Binhassan1Budoor A. Jaber2Shimaa Alazmi3Fatma M.B. Saleman4Sandeep Potluri5Guy Pratt6Christian Ludwig7Douglas G. Ward8Maarten Hoogenkamp9Department of Cancer and Genomics Sciences, School of Medical Sciences, College of Medicine and Health, University of Birmingham, Birmingham B15 2TT, United Kingdom; Birmingham Centre for Genome Biology, University of Birmingham, Birmingham B15 2TTDepartment of Cancer and Genomics Sciences, School of Medical Sciences, College of Medicine and Health, University of Birmingham, Birmingham B15 2TT, United Kingdom; Birmingham Centre for Genome Biology, University of Birmingham, Birmingham B15 2TTDepartment of Cancer and Genomics Sciences, School of Medical Sciences, College of Medicine and Health, University of Birmingham, Birmingham B15 2TTDepartment of Cancer and Genomics Sciences, School of Medical Sciences, College of Medicine and Health, University of Birmingham, Birmingham B15 2TT, United Kingdom; Birmingham Centre for Genome Biology, University of Birmingham, Birmingham B15 2TTDepartment of Cancer and Genomics Sciences, School of Medical Sciences, College of Medicine and Health, University of Birmingham, Birmingham B15 2TT, United Kingdom; Birmingham Centre for Genome Biology, University of Birmingham, Birmingham B15 2TTDepartment of Cancer and Genomics Sciences, School of Medical Sciences, College of Medicine and Health, University of Birmingham, Birmingham B15 2TT, United Kingdom; Birmingham Centre for Genome Biology, University of Birmingham, Birmingham B15 2TTDepartment of Cancer and Genomics Sciences, School of Medical Sciences, College of Medicine and Health, University of Birmingham, Birmingham B15 2TT, United Kingdom; Centre for Clinical Haematology, Queen Elizabeth Hospital Birmingham, BirminghamDepartment of Metabolism and Systems Sciences, School of Medical Sciences, College of Medicine and Health, University of Birmingham, Birmingham B15 2TTDepartment of Cancer and Genomics Sciences, School of Medical Sciences, College of Medicine and Health, University of Birmingham, Birmingham B15 2TTDepartment of Cancer and Genomics Sciences, School of Medical Sciences, College of Medicine and Health, University of Birmingham, Birmingham B15 2TT, United Kingdom; Birmingham Centre for Genome Biology, University of Birmingham, Birmingham B15 2TT The transcriptional mediator LIM domain only 2 (LMO2) forms a large multi-protein complex together with TAL1/LYL1, HEB/E2A, LDB1 and GATA. This complex regulates transcription from the onset of hematopoietic development and during differentiation. Chromosomal rearrangements involving LMO2 and TAL1 are causative for T-cell lymphoblastic leukemia (TALL). We have identified Plant Homeodomain (PHD)-like Finger 6 (PHF6) as a new LMO2 interacting factor. Somatic mutations in PHF6 have been found to occur in several types of leukemia. We show that PHF6 interacts with LMO2 as a part of the TAL1, GATA2, LDB1 complex in T-ALL and binds to the DNA. These findings show that PHF6 associates with the TAL1/LMO2/LDB1/GATA2 complex and regulates genes that have a major role in blood development, such as SPI1 (PU.1). https://haematologica.org/article/view/12167 |
| spellingShingle | Vesna S. Stanulović Sarah Binhassan Budoor A. Jaber Shimaa Alazmi Fatma M.B. Saleman Sandeep Potluri Guy Pratt Christian Ludwig Douglas G. Ward Maarten Hoogenkamp PHF6 interacts with the LMO2 complex in T-cell acute lymphoblastic leukemia Haematologica |
| title | PHF6 interacts with the LMO2 complex in T-cell acute lymphoblastic leukemia |
| title_full | PHF6 interacts with the LMO2 complex in T-cell acute lymphoblastic leukemia |
| title_fullStr | PHF6 interacts with the LMO2 complex in T-cell acute lymphoblastic leukemia |
| title_full_unstemmed | PHF6 interacts with the LMO2 complex in T-cell acute lymphoblastic leukemia |
| title_short | PHF6 interacts with the LMO2 complex in T-cell acute lymphoblastic leukemia |
| title_sort | phf6 interacts with the lmo2 complex in t cell acute lymphoblastic leukemia |
| url | https://haematologica.org/article/view/12167 |
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