Effects of non-thermal plasma on mammalian cells.
Thermal plasmas and lasers have been widely used in medicine to cut, ablate and cauterize tissues through heating; in contrast, non-thermal plasma produces no heat, so its effects can be selective. In order to exploit the potential for clinical applications, including wound healing, sterilization, b...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0016270&type=printable |
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| author | Sameer Kalghatgi Crystal M Kelly Ekaterina Cerchar Behzad Torabi Oleg Alekseev Alexander Fridman Gary Friedman Jane Azizkhan-Clifford |
| author_facet | Sameer Kalghatgi Crystal M Kelly Ekaterina Cerchar Behzad Torabi Oleg Alekseev Alexander Fridman Gary Friedman Jane Azizkhan-Clifford |
| author_sort | Sameer Kalghatgi |
| collection | DOAJ |
| description | Thermal plasmas and lasers have been widely used in medicine to cut, ablate and cauterize tissues through heating; in contrast, non-thermal plasma produces no heat, so its effects can be selective. In order to exploit the potential for clinical applications, including wound healing, sterilization, blood coagulation, and cancer treatment, a mechanistic understanding of the interaction of non-thermal plasma with living tissues is required. Using mammalian cells in culture, it is shown here that non-thermal plasma created by dielectric barrier discharge (DBD) has dose-dependent effects that range from increasing cell proliferation to inducing apoptosis. It is also shown that these effects are primarily due to formation of intracellular reactive oxygen species (ROS). We have utilized γ-H2AX to detect DNA damage induced by non-thermal plasma and found that it is initiated by production of active neutral species that most likely induce formation of organic peroxides in cell medium. Phosphorylation of H2AX following non-thermal plasma treatment is ATR dependent and ATM independent, suggesting that plasma treatment may lead to replication arrest or formation of single-stranded DNA breaks; however, plasma does not lead to formation of bulky adducts/thymine dimers. |
| format | Article |
| id | doaj-art-9cf560a279b74426bcecc3b36e27a25f |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-9cf560a279b74426bcecc3b36e27a25f2025-08-20T03:10:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0161e1627010.1371/journal.pone.0016270Effects of non-thermal plasma on mammalian cells.Sameer KalghatgiCrystal M KellyEkaterina CercharBehzad TorabiOleg AlekseevAlexander FridmanGary FriedmanJane Azizkhan-CliffordThermal plasmas and lasers have been widely used in medicine to cut, ablate and cauterize tissues through heating; in contrast, non-thermal plasma produces no heat, so its effects can be selective. In order to exploit the potential for clinical applications, including wound healing, sterilization, blood coagulation, and cancer treatment, a mechanistic understanding of the interaction of non-thermal plasma with living tissues is required. Using mammalian cells in culture, it is shown here that non-thermal plasma created by dielectric barrier discharge (DBD) has dose-dependent effects that range from increasing cell proliferation to inducing apoptosis. It is also shown that these effects are primarily due to formation of intracellular reactive oxygen species (ROS). We have utilized γ-H2AX to detect DNA damage induced by non-thermal plasma and found that it is initiated by production of active neutral species that most likely induce formation of organic peroxides in cell medium. Phosphorylation of H2AX following non-thermal plasma treatment is ATR dependent and ATM independent, suggesting that plasma treatment may lead to replication arrest or formation of single-stranded DNA breaks; however, plasma does not lead to formation of bulky adducts/thymine dimers.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0016270&type=printable |
| spellingShingle | Sameer Kalghatgi Crystal M Kelly Ekaterina Cerchar Behzad Torabi Oleg Alekseev Alexander Fridman Gary Friedman Jane Azizkhan-Clifford Effects of non-thermal plasma on mammalian cells. PLoS ONE |
| title | Effects of non-thermal plasma on mammalian cells. |
| title_full | Effects of non-thermal plasma on mammalian cells. |
| title_fullStr | Effects of non-thermal plasma on mammalian cells. |
| title_full_unstemmed | Effects of non-thermal plasma on mammalian cells. |
| title_short | Effects of non-thermal plasma on mammalian cells. |
| title_sort | effects of non thermal plasma on mammalian cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0016270&type=printable |
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