Testing activity of antibiotics by agar diffusion
When the quality of antibiotics and antibiotic dosage forms cannot be assessed with the help of chemical methods, one can use two variants of the agar diffusion test described in the State Pharmacopoeia XII, part 1 (General pharmacopoeial monograph 42-006807): the three-doses variant of the agar dif...
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| Format: | Article |
| Language: | Russian |
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Federal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’)
2018-02-01
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| Series: | Регуляторные исследования и экспертиза лекарственных средств |
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| Online Access: | https://www.vedomostincesmp.ru/jour/article/view/46 |
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| _version_ | 1849395127671324672 |
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| author | S. I. Kuleshova |
| author_facet | S. I. Kuleshova |
| author_sort | S. I. Kuleshova |
| collection | DOAJ |
| description | When the quality of antibiotics and antibiotic dosage forms cannot be assessed with the help of chemical methods, one can use two variants of the agar diffusion test described in the State Pharmacopoeia XII, part 1 (General pharmacopoeial monograph 42-006807): the three-doses variant of the agar diffusion test and determination with the help of a standard curve. The method is based on directly proportional relationship between the size of test microorganisms inhibition zones and log concentration (dose) of the antibiotic in the solution placed onto agar bacterial medium. The size of the zone depends on a number of factors: sensitivity of the test microorganism to the antibiotic, homogeneity and density of the bacterial lawn, composition of the growth medium, amount of the growth medium placed on Petri dishes, chemical nature of the antibiotic, the dose of antibiotic used in the experiment. Laboratory employees who use this method to test the quality of antibiotics should have appropriate skills. The activity of the drug under testing is determined by comparing it to the established activity of the reference standard which should have compendial quality. Statistical methods are used to assess obtained results and confirm validity of test conditions. The precision of test results is confirmed by computing the 95% confidence interval, and the repeatability is supported by calculation of the relative standard deviation. |
| format | Article |
| id | doaj-art-9ceab718567f4d15a204e936c9f6a381 |
| institution | Kabale University |
| issn | 3034-3062 3034-3453 |
| language | Russian |
| publishDate | 2018-02-01 |
| publisher | Federal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’) |
| record_format | Article |
| series | Регуляторные исследования и экспертиза лекарственных средств |
| spelling | doaj-art-9ceab718567f4d15a204e936c9f6a3812025-08-20T03:39:45ZrusFederal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’)Регуляторные исследования и экспертиза лекарственных средств3034-30623034-34532018-02-0103131744Testing activity of antibiotics by agar diffusionS. I. Kuleshova0Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» of the Ministry of Health of the Russian FederationWhen the quality of antibiotics and antibiotic dosage forms cannot be assessed with the help of chemical methods, one can use two variants of the agar diffusion test described in the State Pharmacopoeia XII, part 1 (General pharmacopoeial monograph 42-006807): the three-doses variant of the agar diffusion test and determination with the help of a standard curve. The method is based on directly proportional relationship between the size of test microorganisms inhibition zones and log concentration (dose) of the antibiotic in the solution placed onto agar bacterial medium. The size of the zone depends on a number of factors: sensitivity of the test microorganism to the antibiotic, homogeneity and density of the bacterial lawn, composition of the growth medium, amount of the growth medium placed on Petri dishes, chemical nature of the antibiotic, the dose of antibiotic used in the experiment. Laboratory employees who use this method to test the quality of antibiotics should have appropriate skills. The activity of the drug under testing is determined by comparing it to the established activity of the reference standard which should have compendial quality. Statistical methods are used to assess obtained results and confirm validity of test conditions. The precision of test results is confirmed by computing the 95% confidence interval, and the repeatability is supported by calculation of the relative standard deviation.https://www.vedomostincesmp.ru/jour/article/view/46антибиотикиметод диффузии в агарпитательная средатест-микроорганизмлинейная зависимостьбиологическая активностьстандартный образецantibioticsagar diffusionmediumtest microorganismlinear dependencebiological activityreference standard |
| spellingShingle | S. I. Kuleshova Testing activity of antibiotics by agar diffusion Регуляторные исследования и экспертиза лекарственных средств антибиотики метод диффузии в агар питательная среда тест-микроорганизм линейная зависимость биологическая активность стандартный образец antibiotics agar diffusion medium test microorganism linear dependence biological activity reference standard |
| title | Testing activity of antibiotics by agar diffusion |
| title_full | Testing activity of antibiotics by agar diffusion |
| title_fullStr | Testing activity of antibiotics by agar diffusion |
| title_full_unstemmed | Testing activity of antibiotics by agar diffusion |
| title_short | Testing activity of antibiotics by agar diffusion |
| title_sort | testing activity of antibiotics by agar diffusion |
| topic | антибиотики метод диффузии в агар питательная среда тест-микроорганизм линейная зависимость биологическая активность стандартный образец antibiotics agar diffusion medium test microorganism linear dependence biological activity reference standard |
| url | https://www.vedomostincesmp.ru/jour/article/view/46 |
| work_keys_str_mv | AT sikuleshova testingactivityofantibioticsbyagardiffusion |