A Novel Pathogen Detection System Combining a Nucleic Acid Extraction Biochip with a Perovskite Photodetector
The increasing spread of infectious diseases caused by pathogenic microorganisms underscores the urgent need for highly sensitive, portable, and rapid nucleic acid detection technologies to facilitate early diagnosis and effective prevention. In this study, we developed a fluorescence-based nucleic...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/16/5/581 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849327173295407104 |
|---|---|
| author | Zhuo Gao Pan Wang Chang Chen Jian Duan Shilun Feng Bo Liu |
| author_facet | Zhuo Gao Pan Wang Chang Chen Jian Duan Shilun Feng Bo Liu |
| author_sort | Zhuo Gao |
| collection | DOAJ |
| description | The increasing spread of infectious diseases caused by pathogenic microorganisms underscores the urgent need for highly sensitive, portable, and rapid nucleic acid detection technologies to facilitate early diagnosis and effective prevention. In this study, we developed a fluorescence-based nucleic acid detection platform that integrates a microfluidic chip with an all-inorganic perovskite photodetector. The system enables integrated operation of nucleic acid extraction, purification, and amplification on a microfluidic chip, combined with real-time electrical signal readout via a CsPbBr<sub>3</sub> perovskite photodetector. Experimental results indicate that the photodetector exhibits high responsivity at 530 nm, aligning well with the primary emission peak of FAM. The system demonstrates a strong linear correlation between photocurrent and FAM concentration over the range of 0.01–0.4 μM (R<sup>2</sup> = 0.928), with a low detection limit of 0.01 μM and excellent reproducibility across multiple measurements. Validation using FAM standard solutions and Escherichia coli samples confirmed the system’s reliable linearity and signal stability. This platform demonstrates strong potential for rapid pathogen screening and point-of-care diagnostic applications. |
| format | Article |
| id | doaj-art-9ce6edfc64924c8d9ec0c08ea61df723 |
| institution | Kabale University |
| issn | 2072-666X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj-art-9ce6edfc64924c8d9ec0c08ea61df7232025-08-20T03:47:57ZengMDPI AGMicromachines2072-666X2025-05-0116558110.3390/mi16050581A Novel Pathogen Detection System Combining a Nucleic Acid Extraction Biochip with a Perovskite PhotodetectorZhuo Gao0Pan Wang1Chang Chen2Jian Duan3Shilun Feng4Bo Liu5School of Microelectronics, Shanghai University, Shanghai 201800, ChinaState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaSchool of Microelectronics, Shanghai University, Shanghai 201800, ChinaSchool of Microelectronics, Shanghai University, Shanghai 201800, ChinaState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaSchool of Microelectronics, Shanghai University, Shanghai 201800, ChinaThe increasing spread of infectious diseases caused by pathogenic microorganisms underscores the urgent need for highly sensitive, portable, and rapid nucleic acid detection technologies to facilitate early diagnosis and effective prevention. In this study, we developed a fluorescence-based nucleic acid detection platform that integrates a microfluidic chip with an all-inorganic perovskite photodetector. The system enables integrated operation of nucleic acid extraction, purification, and amplification on a microfluidic chip, combined with real-time electrical signal readout via a CsPbBr<sub>3</sub> perovskite photodetector. Experimental results indicate that the photodetector exhibits high responsivity at 530 nm, aligning well with the primary emission peak of FAM. The system demonstrates a strong linear correlation between photocurrent and FAM concentration over the range of 0.01–0.4 μM (R<sup>2</sup> = 0.928), with a low detection limit of 0.01 μM and excellent reproducibility across multiple measurements. Validation using FAM standard solutions and Escherichia coli samples confirmed the system’s reliable linearity and signal stability. This platform demonstrates strong potential for rapid pathogen screening and point-of-care diagnostic applications.https://www.mdpi.com/2072-666X/16/5/581microfluidic chipperovskite photodetectorfluorescence detectionnucleic acid extraction |
| spellingShingle | Zhuo Gao Pan Wang Chang Chen Jian Duan Shilun Feng Bo Liu A Novel Pathogen Detection System Combining a Nucleic Acid Extraction Biochip with a Perovskite Photodetector Micromachines microfluidic chip perovskite photodetector fluorescence detection nucleic acid extraction |
| title | A Novel Pathogen Detection System Combining a Nucleic Acid Extraction Biochip with a Perovskite Photodetector |
| title_full | A Novel Pathogen Detection System Combining a Nucleic Acid Extraction Biochip with a Perovskite Photodetector |
| title_fullStr | A Novel Pathogen Detection System Combining a Nucleic Acid Extraction Biochip with a Perovskite Photodetector |
| title_full_unstemmed | A Novel Pathogen Detection System Combining a Nucleic Acid Extraction Biochip with a Perovskite Photodetector |
| title_short | A Novel Pathogen Detection System Combining a Nucleic Acid Extraction Biochip with a Perovskite Photodetector |
| title_sort | novel pathogen detection system combining a nucleic acid extraction biochip with a perovskite photodetector |
| topic | microfluidic chip perovskite photodetector fluorescence detection nucleic acid extraction |
| url | https://www.mdpi.com/2072-666X/16/5/581 |
| work_keys_str_mv | AT zhuogao anovelpathogendetectionsystemcombininganucleicacidextractionbiochipwithaperovskitephotodetector AT panwang anovelpathogendetectionsystemcombininganucleicacidextractionbiochipwithaperovskitephotodetector AT changchen anovelpathogendetectionsystemcombininganucleicacidextractionbiochipwithaperovskitephotodetector AT jianduan anovelpathogendetectionsystemcombininganucleicacidextractionbiochipwithaperovskitephotodetector AT shilunfeng anovelpathogendetectionsystemcombininganucleicacidextractionbiochipwithaperovskitephotodetector AT boliu anovelpathogendetectionsystemcombininganucleicacidextractionbiochipwithaperovskitephotodetector AT zhuogao novelpathogendetectionsystemcombininganucleicacidextractionbiochipwithaperovskitephotodetector AT panwang novelpathogendetectionsystemcombininganucleicacidextractionbiochipwithaperovskitephotodetector AT changchen novelpathogendetectionsystemcombininganucleicacidextractionbiochipwithaperovskitephotodetector AT jianduan novelpathogendetectionsystemcombininganucleicacidextractionbiochipwithaperovskitephotodetector AT shilunfeng novelpathogendetectionsystemcombininganucleicacidextractionbiochipwithaperovskitephotodetector AT boliu novelpathogendetectionsystemcombininganucleicacidextractionbiochipwithaperovskitephotodetector |