Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal
The mesoniviruses (MESOVs) belong to the newly described Mesoniviridae family (Order: Nidovirales). They have never been reported in Senegal until recently during a study in arbovirus emergence with the detection of a new species of MESOV named Dianke virus (DKV) from common mosquitoes from eastern...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-01-01
|
| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1730710 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850200424522448896 |
|---|---|
| author | Alioune Gaye Moussa Moïse Diagne El Hadji Ndiaye Marie Henriette Dior Ndione Martin Faye Cheikh Talla Gamou Fall Yamar Ba Diawo Diallo Ibrahima Dia Pascal Handschumacher Ousmane Faye Amadou Alpha Sall Mawlouth Diallo |
| author_facet | Alioune Gaye Moussa Moïse Diagne El Hadji Ndiaye Marie Henriette Dior Ndione Martin Faye Cheikh Talla Gamou Fall Yamar Ba Diawo Diallo Ibrahima Dia Pascal Handschumacher Ousmane Faye Amadou Alpha Sall Mawlouth Diallo |
| author_sort | Alioune Gaye |
| collection | DOAJ |
| description | The mesoniviruses (MESOVs) belong to the newly described Mesoniviridae family (Order: Nidovirales). They have never been reported in Senegal until recently during a study in arbovirus emergence with the detection of a new species of MESOV named Dianke virus (DKV) from common mosquitoes from eastern Senegal. Actually, their vector competence for this newly described DKV is unknown. We, therefore, estimated the vector competence of Culex tritaeniorhynchus, Culex quinquefasciatus, Aedes aegypti, and Anopheles gambiae mosquitoes collected in Senegal for DKV using oral infection. Whole bodies, legs/wings, and saliva samples were tested for DKV by RT–PCR to estimate infection, dissemination, and transmission rates. The infectivity of virus particles in the saliva was confirmed by infecting C6/36 cells. Virus transmission rates were up to 95.45% in Culex tritaeniorhynchus, 28% in Cx. quinquefasciatus and 9.09% in Aedes aegypti. Viral particles in the saliva were confirmed infectious by C6/36 cell culture. An. gambiae was able to disseminate DKV only at 20 days post-infection. This study shows that Culex mosquitoes are more competent than Ae. aegypti for DKV, while Anopheles gambiae is not likely a competent vector. |
| format | Article |
| id | doaj-art-9cdd3156a04349b390c366cc6258fffd |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-9cdd3156a04349b390c366cc6258fffd2025-08-20T02:12:20ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-019149650410.1080/22221751.2020.1730710Vector competence of anthropophilic mosquitoes for a new mesonivirus in SenegalAlioune Gaye0Moussa Moïse Diagne1El Hadji Ndiaye2Marie Henriette Dior Ndione3Martin Faye4Cheikh Talla5Gamou Fall6Yamar Ba7Diawo Diallo8Ibrahima Dia9Pascal Handschumacher10Ousmane Faye11Amadou Alpha Sall12Mawlouth Diallo13Unité d’Entomologie Médicale, Institut Pasteur de Dakar, Dakar, SénégalPole de virologie, Unité des Arbovirus et virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, Dakar, SénégalUnité d’Entomologie Médicale, Institut Pasteur de Dakar, Dakar, SénégalPole de virologie, Unité des Arbovirus et virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, Dakar, SénégalPole de virologie, Unité des Arbovirus et virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, Dakar, SénégalEpidemiology of infectious diseases unit, Institut Pasteur de Dakar, Dakar, SenegalPole de virologie, Unité des Arbovirus et virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, Dakar, SénégalUnité d’Entomologie Médicale, Institut Pasteur de Dakar, Dakar, SénégalUnité d’Entomologie Médicale, Institut Pasteur de Dakar, Dakar, SénégalUnité d’Entomologie Médicale, Institut Pasteur de Dakar, Dakar, SénégalAix Marseille Univ, INSERM, IRD, UMR SESSTIM, Sciences Economiques & Sociales de la Santé & Traitement de l'Information Médicale, Marseille, FrancePole de virologie, Unité des Arbovirus et virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, Dakar, SénégalPole de virologie, Unité des Arbovirus et virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, Dakar, SénégalUnité d’Entomologie Médicale, Institut Pasteur de Dakar, Dakar, SénégalThe mesoniviruses (MESOVs) belong to the newly described Mesoniviridae family (Order: Nidovirales). They have never been reported in Senegal until recently during a study in arbovirus emergence with the detection of a new species of MESOV named Dianke virus (DKV) from common mosquitoes from eastern Senegal. Actually, their vector competence for this newly described DKV is unknown. We, therefore, estimated the vector competence of Culex tritaeniorhynchus, Culex quinquefasciatus, Aedes aegypti, and Anopheles gambiae mosquitoes collected in Senegal for DKV using oral infection. Whole bodies, legs/wings, and saliva samples were tested for DKV by RT–PCR to estimate infection, dissemination, and transmission rates. The infectivity of virus particles in the saliva was confirmed by infecting C6/36 cells. Virus transmission rates were up to 95.45% in Culex tritaeniorhynchus, 28% in Cx. quinquefasciatus and 9.09% in Aedes aegypti. Viral particles in the saliva were confirmed infectious by C6/36 cell culture. An. gambiae was able to disseminate DKV only at 20 days post-infection. This study shows that Culex mosquitoes are more competent than Ae. aegypti for DKV, while Anopheles gambiae is not likely a competent vector.https://www.tandfonline.com/doi/10.1080/22221751.2020.1730710Vector competenceDianke virusCulexAedesAnopheles |
| spellingShingle | Alioune Gaye Moussa Moïse Diagne El Hadji Ndiaye Marie Henriette Dior Ndione Martin Faye Cheikh Talla Gamou Fall Yamar Ba Diawo Diallo Ibrahima Dia Pascal Handschumacher Ousmane Faye Amadou Alpha Sall Mawlouth Diallo Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal Emerging Microbes and Infections Vector competence Dianke virus Culex Aedes Anopheles |
| title | Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal |
| title_full | Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal |
| title_fullStr | Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal |
| title_full_unstemmed | Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal |
| title_short | Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal |
| title_sort | vector competence of anthropophilic mosquitoes for a new mesonivirus in senegal |
| topic | Vector competence Dianke virus Culex Aedes Anopheles |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1730710 |
| work_keys_str_mv | AT aliounegaye vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT moussamoisediagne vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT elhadjindiaye vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT mariehenriettediorndione vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT martinfaye vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT cheikhtalla vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT gamoufall vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT yamarba vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT diawodiallo vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT ibrahimadia vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT pascalhandschumacher vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT ousmanefaye vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT amadoualphasall vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal AT mawlouthdiallo vectorcompetenceofanthropophilicmosquitoesforanewmesonivirusinsenegal |