Yiqi Fumai Lyophilized injection protects cardiac contractility in a rat model of heart failure with reduced ejection fraction by modulating SERCA2
Background: Yiqi Fumai Lyophilized Injection (YQFM) is a plant-derived preparation that shows protective effects against cardiac dysfunction in both human and animal models. In heart failure (HF), proteins involved in Ca2+ regulation, such as sarcoplasmic/endoplasmic reticulum Ca2+ transporting prot...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Phytomedicine Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266703132400160X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823861199161262080 |
|---|---|
| author | Yongwei Nie Lu Chen Yanxin Zhang Yuqing Yao Zizhuo Xue Na Guo Meixiu Wan Zhi Li Chunhua Liu Dekun Li Aichun Ju |
| author_facet | Yongwei Nie Lu Chen Yanxin Zhang Yuqing Yao Zizhuo Xue Na Guo Meixiu Wan Zhi Li Chunhua Liu Dekun Li Aichun Ju |
| author_sort | Yongwei Nie |
| collection | DOAJ |
| description | Background: Yiqi Fumai Lyophilized Injection (YQFM) is a plant-derived preparation that shows protective effects against cardiac dysfunction in both human and animal models. In heart failure (HF), proteins involved in Ca2+ regulation, such as sarcoplasmic/endoplasmic reticulum Ca2+ transporting protein 2 (SERCA2) and cardiac ryanodine receptor 2 (RyR2), are dysfunctional. Compensation for the malfunction of these proteins is considered a practical option for restoring heart function. Methods: Coronary artery ligation was used to induce heart failure with reduced ejection fraction (HFrEF) in Sprague-Dawley rats. YQFM was administered via intravenous injection for two weeks. Cardiac contractility was evaluated using echocardiography. Hematoxylin and Eosin (H&E) staining, Masson's trichrome staining, western blotting, and immunohistochemical analysis were performed to evaluate myocardial damage and investigate the underlying molecular mechanisms. In vitro, H9C2 cells were subjected to oxygen and glucose deprivation. Western blotting and immunofluorescence staining were used to assess the expression of key Ca2+-handling proteins in the H9C2 cell models. Results: Twenty-five compounds, mainly ginsenosides, were identified in YQFM. In rats with HFrEF, treatment with YQFM or metoprolol prevents deterioration of cardiac contractility. YQFM caused a patchy delay in the downregulation of SERCA2 in the myocardium, resulting in reduced dilation of the left and right ventricles, thereby preventing left ventricular remodeling. In H9C2 cell models, our results demonstrated that YQFM partially prevented defects in SERCA2. Conclusion: Our study demonstrated that YQFM may function as a positive cardiac contractility modulator and therapeutic agent for cardiac protection by restoring SERCA2 expression in cardiomyocytes in HF after myocardial infarction (MI). |
| format | Article |
| id | doaj-art-9cdb8e0b499c43b492dc79350ae53127 |
| institution | Kabale University |
| issn | 2667-0313 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj-art-9cdb8e0b499c43b492dc79350ae531272025-02-10T04:35:07ZengElsevierPhytomedicine Plus2667-03132025-02-0151100686Yiqi Fumai Lyophilized injection protects cardiac contractility in a rat model of heart failure with reduced ejection fraction by modulating SERCA2Yongwei Nie0Lu Chen1Yanxin Zhang2Yuqing Yao3Zizhuo Xue4Na Guo5Meixiu Wan6Zhi Li7Chunhua Liu8Dekun Li9Aichun Ju10School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, ChinaDepartment of Pharmacy, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia 010017, ChinaTianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Beichen Science and Technology Park, 12 Huaihe Road, Tianjin 300410, China; Tianjin Tasly Pride Pharmaceutical Co., Ltd., 12 Huaihe Road, Tianjin 300410, ChinaTianjin Tasly Pride Pharmaceutical Co., Ltd., 12 Huaihe Road, Tianjin 300410, ChinaGraduate school, Tianjin University of Traditional Chinese Medicine, 10 Poyang Lake Road, Tianjin 301617, ChinaGraduate school, Tianjin University of Traditional Chinese Medicine, 10 Poyang Lake Road, Tianjin 301617, ChinaTianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Beichen Science and Technology Park, 12 Huaihe Road, Tianjin 300410, China; Tianjin Tasly Pride Pharmaceutical Co., Ltd., 12 Huaihe Road, Tianjin 300410, ChinaTianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Beichen Science and Technology Park, 12 Huaihe Road, Tianjin 300410, China; Tianjin Tasly Pride Pharmaceutical Co., Ltd., 12 Huaihe Road, Tianjin 300410, ChinaSchool of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, ChinaTianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Beichen Science and Technology Park, 12 Huaihe Road, Tianjin 300410, China; Tianjin Tasly Pride Pharmaceutical Co., Ltd., 12 Huaihe Road, Tianjin 300410, China; Corresponding authors at: Tianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Beichen Science and Technology Park, Ltd., 12 Huaihe Road, Tianjin 300410, China.Tianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Beichen Science and Technology Park, 12 Huaihe Road, Tianjin 300410, China; Tianjin Tasly Pride Pharmaceutical Co., Ltd., 12 Huaihe Road, Tianjin 300410, China; Corresponding authors at: Tianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Beichen Science and Technology Park, Ltd., 12 Huaihe Road, Tianjin 300410, China.Background: Yiqi Fumai Lyophilized Injection (YQFM) is a plant-derived preparation that shows protective effects against cardiac dysfunction in both human and animal models. In heart failure (HF), proteins involved in Ca2+ regulation, such as sarcoplasmic/endoplasmic reticulum Ca2+ transporting protein 2 (SERCA2) and cardiac ryanodine receptor 2 (RyR2), are dysfunctional. Compensation for the malfunction of these proteins is considered a practical option for restoring heart function. Methods: Coronary artery ligation was used to induce heart failure with reduced ejection fraction (HFrEF) in Sprague-Dawley rats. YQFM was administered via intravenous injection for two weeks. Cardiac contractility was evaluated using echocardiography. Hematoxylin and Eosin (H&E) staining, Masson's trichrome staining, western blotting, and immunohistochemical analysis were performed to evaluate myocardial damage and investigate the underlying molecular mechanisms. In vitro, H9C2 cells were subjected to oxygen and glucose deprivation. Western blotting and immunofluorescence staining were used to assess the expression of key Ca2+-handling proteins in the H9C2 cell models. Results: Twenty-five compounds, mainly ginsenosides, were identified in YQFM. In rats with HFrEF, treatment with YQFM or metoprolol prevents deterioration of cardiac contractility. YQFM caused a patchy delay in the downregulation of SERCA2 in the myocardium, resulting in reduced dilation of the left and right ventricles, thereby preventing left ventricular remodeling. In H9C2 cell models, our results demonstrated that YQFM partially prevented defects in SERCA2. Conclusion: Our study demonstrated that YQFM may function as a positive cardiac contractility modulator and therapeutic agent for cardiac protection by restoring SERCA2 expression in cardiomyocytes in HF after myocardial infarction (MI).http://www.sciencedirect.com/science/article/pii/S266703132400160XYQFMHFSERCA2cardiac contractilitycardiac remodeling |
| spellingShingle | Yongwei Nie Lu Chen Yanxin Zhang Yuqing Yao Zizhuo Xue Na Guo Meixiu Wan Zhi Li Chunhua Liu Dekun Li Aichun Ju Yiqi Fumai Lyophilized injection protects cardiac contractility in a rat model of heart failure with reduced ejection fraction by modulating SERCA2 Phytomedicine Plus YQFM HF SERCA2 cardiac contractility cardiac remodeling |
| title | Yiqi Fumai Lyophilized injection protects cardiac contractility in a rat model of heart failure with reduced ejection fraction by modulating SERCA2 |
| title_full | Yiqi Fumai Lyophilized injection protects cardiac contractility in a rat model of heart failure with reduced ejection fraction by modulating SERCA2 |
| title_fullStr | Yiqi Fumai Lyophilized injection protects cardiac contractility in a rat model of heart failure with reduced ejection fraction by modulating SERCA2 |
| title_full_unstemmed | Yiqi Fumai Lyophilized injection protects cardiac contractility in a rat model of heart failure with reduced ejection fraction by modulating SERCA2 |
| title_short | Yiqi Fumai Lyophilized injection protects cardiac contractility in a rat model of heart failure with reduced ejection fraction by modulating SERCA2 |
| title_sort | yiqi fumai lyophilized injection protects cardiac contractility in a rat model of heart failure with reduced ejection fraction by modulating serca2 |
| topic | YQFM HF SERCA2 cardiac contractility cardiac remodeling |
| url | http://www.sciencedirect.com/science/article/pii/S266703132400160X |
| work_keys_str_mv | AT yongweinie yiqifumailyophilizedinjectionprotectscardiaccontractilityinaratmodelofheartfailurewithreducedejectionfractionbymodulatingserca2 AT luchen yiqifumailyophilizedinjectionprotectscardiaccontractilityinaratmodelofheartfailurewithreducedejectionfractionbymodulatingserca2 AT yanxinzhang yiqifumailyophilizedinjectionprotectscardiaccontractilityinaratmodelofheartfailurewithreducedejectionfractionbymodulatingserca2 AT yuqingyao yiqifumailyophilizedinjectionprotectscardiaccontractilityinaratmodelofheartfailurewithreducedejectionfractionbymodulatingserca2 AT zizhuoxue yiqifumailyophilizedinjectionprotectscardiaccontractilityinaratmodelofheartfailurewithreducedejectionfractionbymodulatingserca2 AT naguo yiqifumailyophilizedinjectionprotectscardiaccontractilityinaratmodelofheartfailurewithreducedejectionfractionbymodulatingserca2 AT meixiuwan yiqifumailyophilizedinjectionprotectscardiaccontractilityinaratmodelofheartfailurewithreducedejectionfractionbymodulatingserca2 AT zhili yiqifumailyophilizedinjectionprotectscardiaccontractilityinaratmodelofheartfailurewithreducedejectionfractionbymodulatingserca2 AT chunhualiu yiqifumailyophilizedinjectionprotectscardiaccontractilityinaratmodelofheartfailurewithreducedejectionfractionbymodulatingserca2 AT dekunli yiqifumailyophilizedinjectionprotectscardiaccontractilityinaratmodelofheartfailurewithreducedejectionfractionbymodulatingserca2 AT aichunju yiqifumailyophilizedinjectionprotectscardiaccontractilityinaratmodelofheartfailurewithreducedejectionfractionbymodulatingserca2 |