CDK12 Promotes Cervical Cancer Progression through Enhancing Macrophage Infiltration
Cervical cancer (CC) is a commonly diagnosed and primary consideration of cancer patient death in female reproductive system malignancy. Cyclin-dependent kinase 12 (CDK12), as a transcription-associated CDK, plays important roles in tumor-promoting behaviors, whereas the underlying mechanisms of CDK...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/6645885 |
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| author | Bikang Yang Jing Chen Yincheng Teng |
| author_facet | Bikang Yang Jing Chen Yincheng Teng |
| author_sort | Bikang Yang |
| collection | DOAJ |
| description | Cervical cancer (CC) is a commonly diagnosed and primary consideration of cancer patient death in female reproductive system malignancy. Cyclin-dependent kinase 12 (CDK12), as a transcription-associated CDK, plays important roles in tumor-promoting behaviors, whereas the underlying mechanisms of CDK12 in CC progression are still obscure. In this report, we investigated the role of CDK12 in cervical cancer. The current study identified CDK12 mRNA and protein expression remarkably upregulated in CC patients. Upregulated CDK12 was closely associated with CC progression and poor prognosis. In vitro and in vivo functional experiments showed that knockdown of CDK12 inhibited cancer cell proliferation and colony formation and promoted apoptosis. Further investigations demonstrated that CDK12 regulated the immune microenvironment to facilitate the progression of CC cells by promoting macrophage infiltration. Meanwhile, we first demonstrated that nuclear import of CDK12 is mediated by TNPO1 and might be a new therapeutic target in oncology. Collectively, this study pointed out the potential of CDK12 to serve as a novel therapeutic target in restricting CC proliferation and cell cycle process through promoting macrophage infiltration. |
| format | Article |
| id | doaj-art-9ccc037941d44a02a9d32d9fdbcb87e2 |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-9ccc037941d44a02a9d32d9fdbcb87e22025-08-20T02:20:19ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/66458856645885CDK12 Promotes Cervical Cancer Progression through Enhancing Macrophage InfiltrationBikang Yang0Jing Chen1Yincheng Teng2Department of Gynecology and Obstetrics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, ChinaDepartment of Gynecology and Obstetrics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, ChinaDepartment of Gynecology and Obstetrics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, ChinaCervical cancer (CC) is a commonly diagnosed and primary consideration of cancer patient death in female reproductive system malignancy. Cyclin-dependent kinase 12 (CDK12), as a transcription-associated CDK, plays important roles in tumor-promoting behaviors, whereas the underlying mechanisms of CDK12 in CC progression are still obscure. In this report, we investigated the role of CDK12 in cervical cancer. The current study identified CDK12 mRNA and protein expression remarkably upregulated in CC patients. Upregulated CDK12 was closely associated with CC progression and poor prognosis. In vitro and in vivo functional experiments showed that knockdown of CDK12 inhibited cancer cell proliferation and colony formation and promoted apoptosis. Further investigations demonstrated that CDK12 regulated the immune microenvironment to facilitate the progression of CC cells by promoting macrophage infiltration. Meanwhile, we first demonstrated that nuclear import of CDK12 is mediated by TNPO1 and might be a new therapeutic target in oncology. Collectively, this study pointed out the potential of CDK12 to serve as a novel therapeutic target in restricting CC proliferation and cell cycle process through promoting macrophage infiltration.http://dx.doi.org/10.1155/2021/6645885 |
| spellingShingle | Bikang Yang Jing Chen Yincheng Teng CDK12 Promotes Cervical Cancer Progression through Enhancing Macrophage Infiltration Journal of Immunology Research |
| title | CDK12 Promotes Cervical Cancer Progression through Enhancing Macrophage Infiltration |
| title_full | CDK12 Promotes Cervical Cancer Progression through Enhancing Macrophage Infiltration |
| title_fullStr | CDK12 Promotes Cervical Cancer Progression through Enhancing Macrophage Infiltration |
| title_full_unstemmed | CDK12 Promotes Cervical Cancer Progression through Enhancing Macrophage Infiltration |
| title_short | CDK12 Promotes Cervical Cancer Progression through Enhancing Macrophage Infiltration |
| title_sort | cdk12 promotes cervical cancer progression through enhancing macrophage infiltration |
| url | http://dx.doi.org/10.1155/2021/6645885 |
| work_keys_str_mv | AT bikangyang cdk12promotescervicalcancerprogressionthroughenhancingmacrophageinfiltration AT jingchen cdk12promotescervicalcancerprogressionthroughenhancingmacrophageinfiltration AT yinchengteng cdk12promotescervicalcancerprogressionthroughenhancingmacrophageinfiltration |