LncRNA RNF144A-AS1 gene polymorphisms and their influence on lung cancer patients in the Chinese Han population
Lung cancer is primarily classified as NSCLC, which is distinguished by a wide range of genetic variations. This study focused on RNF144A-AS1, a relatively unexplored lncRNA, to explore the impact of its genetic polymorphisms on the susceptibility to NSCLC. We detected RNF144A-AS1 expression and its...
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KeAi Communications Co., Ltd.
2025-02-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054024001513 |
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| author | Hongjiao Wu Yuning Xie Ang Li Xiyao Liu Liwen Guo Fengjun Wu Zhenbang Yang Zhi Zhang Xuemei Zhang |
| author_facet | Hongjiao Wu Yuning Xie Ang Li Xiyao Liu Liwen Guo Fengjun Wu Zhenbang Yang Zhi Zhang Xuemei Zhang |
| author_sort | Hongjiao Wu |
| collection | DOAJ |
| description | Lung cancer is primarily classified as NSCLC, which is distinguished by a wide range of genetic variations. This study focused on RNF144A-AS1, a relatively unexplored lncRNA, to explore the impact of its genetic polymorphisms on the susceptibility to NSCLC. We detected RNF144A-AS1 expression and its correlation with prognosis and clinical pathological features using bioinformatics analysis. The association between RNF144A-AS1 polymorphism and NSCLC susceptibility was evaluated using case-control methods. This investigation featured a cohort of 700 NSCLC individuals and 700 healthy controls. The genotype of genetic variation was detected by PCR-RFLP and iMLDR, followed by subsequent calculation of OR and 95 % CI. Our data show that RNF144A-AS1 exhibits high expression levels in LUAD tissues and its expression is closely linked to LUAD progression and prognosis. Carrier of RNF144A-AS1 rs3806609 TT genotype increased NSCLC susceptibility compared to carrier of rs3806609 CC genotype (OR = 2.21, 95%CI = 1.57–3.13). Our study identifies RNF144A-AS1 genetic variants as potential susceptibility markers in NSCLC. RNF144A-AS1 promotes cell proliferation and migration in LUAD through the IFN-γ/JAK2/STAT1 signalling pathway. Collectively, these findings pave the way for developing targeted therapies and diagnostic tools based on RNF144A-AS1 and its variants. |
| format | Article |
| id | doaj-art-9cb4ddf196064ad2baf45688770a3e4b |
| institution | OA Journals |
| issn | 2468-0540 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Non-coding RNA Research |
| spelling | doaj-art-9cb4ddf196064ad2baf45688770a3e4b2025-08-20T02:04:38ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402025-02-011025226010.1016/j.ncrna.2024.10.008LncRNA RNF144A-AS1 gene polymorphisms and their influence on lung cancer patients in the Chinese Han populationHongjiao Wu0Yuning Xie1Ang Li2Xiyao Liu3Liwen Guo4Fengjun Wu5Zhenbang Yang6Zhi Zhang7Xuemei Zhang8School of Public Health, North China University of Science and Technology, Tangshan, 063210, China; College of Life Science, North China University of Science and Technology, Tangshan, 063210, China; Hebei Key Laboratory of Occupational Health and Safety for Coal Industry, Tangshan, 063210, ChinaSchool of Public Health, North China University of Science and Technology, Tangshan, 063210, ChinaSchool of Public Health, North China University of Science and Technology, Tangshan, 063210, ChinaCollege of Life Science, North China University of Science and Technology, Tangshan, 063210, ChinaCollege of Life Science, North China University of Science and Technology, Tangshan, 063210, ChinaCollege of Life Science, North China University of Science and Technology, Tangshan, 063210, ChinaSchool of Basic Medical Sciences, North China University of Science and Technology, Tangshan, 063210, ChinaAffiliated Tangshan Gongren Hospital, North China University of Science and Technology, Tangshan, 063000, ChinaSchool of Public Health, North China University of Science and Technology, Tangshan, 063210, China; College of Life Science, North China University of Science and Technology, Tangshan, 063210, China; Hebei Key Laboratory of Occupational Health and Safety for Coal Industry, Tangshan, 063210, China; Corresponding author. College of Life Science, North China University of Science and Technology, Tangshan, 063210, China.Lung cancer is primarily classified as NSCLC, which is distinguished by a wide range of genetic variations. This study focused on RNF144A-AS1, a relatively unexplored lncRNA, to explore the impact of its genetic polymorphisms on the susceptibility to NSCLC. We detected RNF144A-AS1 expression and its correlation with prognosis and clinical pathological features using bioinformatics analysis. The association between RNF144A-AS1 polymorphism and NSCLC susceptibility was evaluated using case-control methods. This investigation featured a cohort of 700 NSCLC individuals and 700 healthy controls. The genotype of genetic variation was detected by PCR-RFLP and iMLDR, followed by subsequent calculation of OR and 95 % CI. Our data show that RNF144A-AS1 exhibits high expression levels in LUAD tissues and its expression is closely linked to LUAD progression and prognosis. Carrier of RNF144A-AS1 rs3806609 TT genotype increased NSCLC susceptibility compared to carrier of rs3806609 CC genotype (OR = 2.21, 95%CI = 1.57–3.13). Our study identifies RNF144A-AS1 genetic variants as potential susceptibility markers in NSCLC. RNF144A-AS1 promotes cell proliferation and migration in LUAD through the IFN-γ/JAK2/STAT1 signalling pathway. Collectively, these findings pave the way for developing targeted therapies and diagnostic tools based on RNF144A-AS1 and its variants.http://www.sciencedirect.com/science/article/pii/S2468054024001513RNF144A-AS1NSCLCPolymorphismBiomarkers |
| spellingShingle | Hongjiao Wu Yuning Xie Ang Li Xiyao Liu Liwen Guo Fengjun Wu Zhenbang Yang Zhi Zhang Xuemei Zhang LncRNA RNF144A-AS1 gene polymorphisms and their influence on lung cancer patients in the Chinese Han population Non-coding RNA Research RNF144A-AS1 NSCLC Polymorphism Biomarkers |
| title | LncRNA RNF144A-AS1 gene polymorphisms and their influence on lung cancer patients in the Chinese Han population |
| title_full | LncRNA RNF144A-AS1 gene polymorphisms and their influence on lung cancer patients in the Chinese Han population |
| title_fullStr | LncRNA RNF144A-AS1 gene polymorphisms and their influence on lung cancer patients in the Chinese Han population |
| title_full_unstemmed | LncRNA RNF144A-AS1 gene polymorphisms and their influence on lung cancer patients in the Chinese Han population |
| title_short | LncRNA RNF144A-AS1 gene polymorphisms and their influence on lung cancer patients in the Chinese Han population |
| title_sort | lncrna rnf144a as1 gene polymorphisms and their influence on lung cancer patients in the chinese han population |
| topic | RNF144A-AS1 NSCLC Polymorphism Biomarkers |
| url | http://www.sciencedirect.com/science/article/pii/S2468054024001513 |
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