Pharmacological assessment of the extract and a novel compound of Bacillus velezensis DM derived from the rhizosphere of Datura metel L. with microbial molecular screening
Abstract Background Rhizosphere bacteria were considered a prospective reservoir of bioactive compounds with significant pharmacological efficacy. Methods From the rhizosphere of Datura metel L., Bacillus velezensis DM was isolated and characterized using 16 S rRNA. PCR screening and sequencing were...
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BMC
2025-04-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-025-04879-x |
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| author | Mohamed A. Awad Shahenda Mahgoub Hesham S. M. Soliman Sherif F. Hammad |
| author_facet | Mohamed A. Awad Shahenda Mahgoub Hesham S. M. Soliman Sherif F. Hammad |
| author_sort | Mohamed A. Awad |
| collection | DOAJ |
| description | Abstract Background Rhizosphere bacteria were considered a prospective reservoir of bioactive compounds with significant pharmacological efficacy. Methods From the rhizosphere of Datura metel L., Bacillus velezensis DM was isolated and characterized using 16 S rRNA. PCR screening and sequencing were conducted to identify genes related to bioactive metabolite production. The extraction of secondary metabolites from the bacterial strain was performed via a fermentation process. The ethyl acetate extract of the propagated strain was subjected to fractionation and purification through various chromatographic techniques. The characterization of the isolated compounds was accomplished using different spectroscopic methods, such as 1D and 2D-NMR. An MTT test was conducted to assess the cytotoxic activity of bacterial extract on MCF-7, HepG-2, and HCT-116 cells. Furthermore, its pure compound (1) was tested for its cytotoxicity on HCT-116 and a normal cell (THLE2) to test its safety for normal cells. Apoptosis was identified through flow cytometry on HCT-116 cells after double-staining with PI and annexin V-FITC. The antioxidant action of bacterial extract was assessed through DPPH and ABTS assays. Furthermore, anti-inflammatory evaluations were carried out employing lipoxygenase (5-LOX) and cyclooxygenase (COX-2) inhibition. Results The NCBI GenBank database has effectively incorporated the 16 S rRNA gene sequence of Bacillus velezensis DM under the accession number OR364492. Polyketide synthase and two lipopeptide genes for surfactin and iturin A were effectively detected by PCR, and their sequences were included in the Genbank database. A novel compound, 5,6-di(methylamino)hex-5-ene-1,2,3-triol (1), was successfully separated from the strain. Bacterial extract demonstrated significant cytotoxic activity against the evaluated cancer cells, exhibiting the most pronounced effect on HCT-116 cells. Compound (1) showed promising cytotoxic potential against HCT-116 cells with a higher selectivity index (2.5) towards cancer cells in comparison to Doxorubicin (1.49). Apoptosis assay showed that bacterial extract caused apoptosis about 14 folds compared to the control HCT-116 cells. Furthermore, it showed a potent anti-inflammatory outcome (IC50 = 1.927 µg/mL) and antioxidant activity at IC50 of 76.8 µg/mL. Conclusion This study revealed the possible pharmacological effects of secondary metabolites generated by Bacillus velezensis DM, making it a valuable resource for isolating bioactive compounds with potential therapeutic and biomedical uses. |
| format | Article |
| id | doaj-art-9cb29fccba0f466bb3add092c38f57a2 |
| institution | OA Journals |
| issn | 2662-7671 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
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| series | BMC Complementary Medicine and Therapies |
| spelling | doaj-art-9cb29fccba0f466bb3add092c38f57a22025-08-20T02:30:19ZengBMCBMC Complementary Medicine and Therapies2662-76712025-04-0125111710.1186/s12906-025-04879-xPharmacological assessment of the extract and a novel compound of Bacillus velezensis DM derived from the rhizosphere of Datura metel L. with microbial molecular screeningMohamed A. Awad0Shahenda Mahgoub1Hesham S. M. Soliman2Sherif F. Hammad3Biotechnology Program, Institute of Basic and Applied Science, Egypt-Japan University of Science and Technology (E-JUST)Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Helwan UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Helwan UniversityDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Helwan UniversityAbstract Background Rhizosphere bacteria were considered a prospective reservoir of bioactive compounds with significant pharmacological efficacy. Methods From the rhizosphere of Datura metel L., Bacillus velezensis DM was isolated and characterized using 16 S rRNA. PCR screening and sequencing were conducted to identify genes related to bioactive metabolite production. The extraction of secondary metabolites from the bacterial strain was performed via a fermentation process. The ethyl acetate extract of the propagated strain was subjected to fractionation and purification through various chromatographic techniques. The characterization of the isolated compounds was accomplished using different spectroscopic methods, such as 1D and 2D-NMR. An MTT test was conducted to assess the cytotoxic activity of bacterial extract on MCF-7, HepG-2, and HCT-116 cells. Furthermore, its pure compound (1) was tested for its cytotoxicity on HCT-116 and a normal cell (THLE2) to test its safety for normal cells. Apoptosis was identified through flow cytometry on HCT-116 cells after double-staining with PI and annexin V-FITC. The antioxidant action of bacterial extract was assessed through DPPH and ABTS assays. Furthermore, anti-inflammatory evaluations were carried out employing lipoxygenase (5-LOX) and cyclooxygenase (COX-2) inhibition. Results The NCBI GenBank database has effectively incorporated the 16 S rRNA gene sequence of Bacillus velezensis DM under the accession number OR364492. Polyketide synthase and two lipopeptide genes for surfactin and iturin A were effectively detected by PCR, and their sequences were included in the Genbank database. A novel compound, 5,6-di(methylamino)hex-5-ene-1,2,3-triol (1), was successfully separated from the strain. Bacterial extract demonstrated significant cytotoxic activity against the evaluated cancer cells, exhibiting the most pronounced effect on HCT-116 cells. Compound (1) showed promising cytotoxic potential against HCT-116 cells with a higher selectivity index (2.5) towards cancer cells in comparison to Doxorubicin (1.49). Apoptosis assay showed that bacterial extract caused apoptosis about 14 folds compared to the control HCT-116 cells. Furthermore, it showed a potent anti-inflammatory outcome (IC50 = 1.927 µg/mL) and antioxidant activity at IC50 of 76.8 µg/mL. Conclusion This study revealed the possible pharmacological effects of secondary metabolites generated by Bacillus velezensis DM, making it a valuable resource for isolating bioactive compounds with potential therapeutic and biomedical uses.https://doi.org/10.1186/s12906-025-04879-xDatura metel L.Rhizosphere bacteriaBacillus velezensis DMBiosynthetic genesIn vitro pharmacology |
| spellingShingle | Mohamed A. Awad Shahenda Mahgoub Hesham S. M. Soliman Sherif F. Hammad Pharmacological assessment of the extract and a novel compound of Bacillus velezensis DM derived from the rhizosphere of Datura metel L. with microbial molecular screening BMC Complementary Medicine and Therapies Datura metel L. Rhizosphere bacteria Bacillus velezensis DM Biosynthetic genes In vitro pharmacology |
| title | Pharmacological assessment of the extract and a novel compound of Bacillus velezensis DM derived from the rhizosphere of Datura metel L. with microbial molecular screening |
| title_full | Pharmacological assessment of the extract and a novel compound of Bacillus velezensis DM derived from the rhizosphere of Datura metel L. with microbial molecular screening |
| title_fullStr | Pharmacological assessment of the extract and a novel compound of Bacillus velezensis DM derived from the rhizosphere of Datura metel L. with microbial molecular screening |
| title_full_unstemmed | Pharmacological assessment of the extract and a novel compound of Bacillus velezensis DM derived from the rhizosphere of Datura metel L. with microbial molecular screening |
| title_short | Pharmacological assessment of the extract and a novel compound of Bacillus velezensis DM derived from the rhizosphere of Datura metel L. with microbial molecular screening |
| title_sort | pharmacological assessment of the extract and a novel compound of bacillus velezensis dm derived from the rhizosphere of datura metel l with microbial molecular screening |
| topic | Datura metel L. Rhizosphere bacteria Bacillus velezensis DM Biosynthetic genes In vitro pharmacology |
| url | https://doi.org/10.1186/s12906-025-04879-x |
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