Phytochemicals from Pyrrosia longifolia (Burm. f.) C.V. Morton with antibacterial activity
Background and purpose: Pyrrosia longifolia is one of the medicinal plants in Indonesia. However, it has received little attention regarding pharmacological properties and phytochemicals. This study aimed to isolate bioactive compounds and evaluate their antibacterial activities. Experimental approa...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Research in Pharmaceutical Sciences |
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| Online Access: | https://journals.lww.com/10.4103/RPS.RPS_151_23 |
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| author | Hilwan Yuda Teruna Rohimatul Khodijah Neni Frimayanti Rudi Hendra |
| author_facet | Hilwan Yuda Teruna Rohimatul Khodijah Neni Frimayanti Rudi Hendra |
| author_sort | Hilwan Yuda Teruna |
| collection | DOAJ |
| description | Background and purpose:
Pyrrosia longifolia is one of the medicinal plants in Indonesia. However, it has received little attention regarding pharmacological properties and phytochemicals. This study aimed to isolate bioactive compounds and evaluate their antibacterial activities.
Experimental approach:
The secondary metabolites were isolated using a bioassay-guided approach. The aerial part was macerated in methanol and the crude methanol was partitioned with organic solvents to obtain n-hexane, dichloromethane, ethyl acetate, and water extracts. The ethyl acetate extract was purified using chromatography procedures, yielding six chemicals, and their structures were determined using spectroscopy. The antibacterial activity of the compounds was evaluated.
Findings/Results:
Six secondary phytochemical metabolites were identified including naringin (1), catechin (2), quercetin (3), rutin (4), kaempferol (5), and mangiferin (6). The absolute configurations of compounds 1 and 2 were ascertained using electronic circular dichroism as 2S for naringin and 2R, 3S for catechin. The compounds exhibited antibacterial activity against several pathogenic bacteria, with MIC and MBC values ranging from 7.8 to 250 μg/mL. Computational investigations of these compounds revealed a substantial affinity for bacterial receptors’ active and allosteric regions. Furthermore, rutin exhibited the capacity to reduce the activity of β-ketoacyl-acyl carrier protein synthase III, enhancing its antibacterial effectiveness.
Conclusion and implications:
The in-vitro assessment revealed that the six identified compounds possess a wide range of antibacterial activity, which was corroborated by in-silico analyses. However, further investigation is required to back up the conclusion of this study. |
| format | Article |
| id | doaj-art-9cac0217f3604990b44017d51f900f36 |
| institution | Kabale University |
| issn | 1735-5362 1735-9414 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Research in Pharmaceutical Sciences |
| spelling | doaj-art-9cac0217f3604990b44017d51f900f362025-01-07T09:56:57ZengWolters Kluwer Medknow PublicationsResearch in Pharmaceutical Sciences1735-53621735-94142024-12-0119671272810.4103/RPS.RPS_151_23Phytochemicals from Pyrrosia longifolia (Burm. f.) C.V. Morton with antibacterial activityHilwan Yuda TerunaRohimatul KhodijahNeni FrimayantiRudi HendraBackground and purpose: Pyrrosia longifolia is one of the medicinal plants in Indonesia. However, it has received little attention regarding pharmacological properties and phytochemicals. This study aimed to isolate bioactive compounds and evaluate their antibacterial activities. Experimental approach: The secondary metabolites were isolated using a bioassay-guided approach. The aerial part was macerated in methanol and the crude methanol was partitioned with organic solvents to obtain n-hexane, dichloromethane, ethyl acetate, and water extracts. The ethyl acetate extract was purified using chromatography procedures, yielding six chemicals, and their structures were determined using spectroscopy. The antibacterial activity of the compounds was evaluated. Findings/Results: Six secondary phytochemical metabolites were identified including naringin (1), catechin (2), quercetin (3), rutin (4), kaempferol (5), and mangiferin (6). The absolute configurations of compounds 1 and 2 were ascertained using electronic circular dichroism as 2S for naringin and 2R, 3S for catechin. The compounds exhibited antibacterial activity against several pathogenic bacteria, with MIC and MBC values ranging from 7.8 to 250 μg/mL. Computational investigations of these compounds revealed a substantial affinity for bacterial receptors’ active and allosteric regions. Furthermore, rutin exhibited the capacity to reduce the activity of β-ketoacyl-acyl carrier protein synthase III, enhancing its antibacterial effectiveness. Conclusion and implications: The in-vitro assessment revealed that the six identified compounds possess a wide range of antibacterial activity, which was corroborated by in-silico analyses. However, further investigation is required to back up the conclusion of this study.https://journals.lww.com/10.4103/RPS.RPS_151_23phenolic antibacterial compoundspolypodiaceaepyrrosia longifoliasecondary metabolites isolationpdb id: 2zdq1hnj3il7 |
| spellingShingle | Hilwan Yuda Teruna Rohimatul Khodijah Neni Frimayanti Rudi Hendra Phytochemicals from Pyrrosia longifolia (Burm. f.) C.V. Morton with antibacterial activity Research in Pharmaceutical Sciences phenolic antibacterial compounds polypodiaceae pyrrosia longifolia secondary metabolites isolation pdb id: 2zdq 1hnj 3il7 |
| title | Phytochemicals from Pyrrosia longifolia (Burm. f.) C.V. Morton with antibacterial activity |
| title_full | Phytochemicals from Pyrrosia longifolia (Burm. f.) C.V. Morton with antibacterial activity |
| title_fullStr | Phytochemicals from Pyrrosia longifolia (Burm. f.) C.V. Morton with antibacterial activity |
| title_full_unstemmed | Phytochemicals from Pyrrosia longifolia (Burm. f.) C.V. Morton with antibacterial activity |
| title_short | Phytochemicals from Pyrrosia longifolia (Burm. f.) C.V. Morton with antibacterial activity |
| title_sort | phytochemicals from pyrrosia longifolia burm f c v morton with antibacterial activity |
| topic | phenolic antibacterial compounds polypodiaceae pyrrosia longifolia secondary metabolites isolation pdb id: 2zdq 1hnj 3il7 |
| url | https://journals.lww.com/10.4103/RPS.RPS_151_23 |
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