Detection of Bovine Milk Adulteration in Non-novine Milk Using Real-Time Polymerase Chain Reaction
In this study, adulteration of bovine milk in fresh and thermally processed non-bovine milk was detected by SYBR Green and TaqMan real-time polymerase chain reaction (real-time PCR), and the effect of different heat treatment methods on the adulteration detection was explored aiming to meet the requ...
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| Language: | English |
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China Food Publishing Company
2025-03-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-6-030.pdf |
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| author | LI Wei, SUN Guodong, LU Jiarong, ZHAO Zhongkai, YANG Jie |
| author_facet | LI Wei, SUN Guodong, LU Jiarong, ZHAO Zhongkai, YANG Jie |
| author_sort | LI Wei, SUN Guodong, LU Jiarong, ZHAO Zhongkai, YANG Jie |
| collection | DOAJ |
| description | In this study, adulteration of bovine milk in fresh and thermally processed non-bovine milk was detected by SYBR Green and TaqMan real-time polymerase chain reaction (real-time PCR), and the effect of different heat treatment methods on the adulteration detection was explored aiming to meet the requirements for the detection of adulteration of different commercial non-bovine milk products. The results showed that the designed bovine-specific primers allowed specific amplification of DNA in bovine milk without cross-reactivity with DNA from non-target animals. The minimum detection limits of SYBR Green and TaqMan real-time PCR for bovine milk DNA were 1 and 10 pg, respectively, and both methods were capable of detecting at least 0.1% (m/m) bovine milk adulteration in non-bovine milk mixtures. The results of nine repeated experiments over three days confirmed high reproducibility of the methods. In addition, using the real-time PCR methods, bovine milk was detected in 7 out of 20 commercial non-bovine milk products, indicating high resolution and great practical application value of these methods. |
| format | Article |
| id | doaj-art-9c8c986efda54ee0b2743bc5ac63a407 |
| institution | OA Journals |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-9c8c986efda54ee0b2743bc5ac63a4072025-08-20T02:27:06ZengChina Food Publishing CompanyShipin Kexue1002-66302025-03-0146626327410.7506/spkx1002-6630-20240727-273Detection of Bovine Milk Adulteration in Non-novine Milk Using Real-Time Polymerase Chain ReactionLI Wei, SUN Guodong, LU Jiarong, ZHAO Zhongkai, YANG Jie0(1. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, ürümqi 830017, China; 2. Xinjiang Camel Industry Engineering and Technology Research Center, ürümqi 830017, China)In this study, adulteration of bovine milk in fresh and thermally processed non-bovine milk was detected by SYBR Green and TaqMan real-time polymerase chain reaction (real-time PCR), and the effect of different heat treatment methods on the adulteration detection was explored aiming to meet the requirements for the detection of adulteration of different commercial non-bovine milk products. The results showed that the designed bovine-specific primers allowed specific amplification of DNA in bovine milk without cross-reactivity with DNA from non-target animals. The minimum detection limits of SYBR Green and TaqMan real-time PCR for bovine milk DNA were 1 and 10 pg, respectively, and both methods were capable of detecting at least 0.1% (m/m) bovine milk adulteration in non-bovine milk mixtures. The results of nine repeated experiments over three days confirmed high reproducibility of the methods. In addition, using the real-time PCR methods, bovine milk was detected in 7 out of 20 commercial non-bovine milk products, indicating high resolution and great practical application value of these methods.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-6-030.pdfnon-bovine milk; real-time polymerase chain reaction; food adulteration; authenticity analysis |
| spellingShingle | LI Wei, SUN Guodong, LU Jiarong, ZHAO Zhongkai, YANG Jie Detection of Bovine Milk Adulteration in Non-novine Milk Using Real-Time Polymerase Chain Reaction Shipin Kexue non-bovine milk; real-time polymerase chain reaction; food adulteration; authenticity analysis |
| title | Detection of Bovine Milk Adulteration in Non-novine Milk Using Real-Time Polymerase Chain Reaction |
| title_full | Detection of Bovine Milk Adulteration in Non-novine Milk Using Real-Time Polymerase Chain Reaction |
| title_fullStr | Detection of Bovine Milk Adulteration in Non-novine Milk Using Real-Time Polymerase Chain Reaction |
| title_full_unstemmed | Detection of Bovine Milk Adulteration in Non-novine Milk Using Real-Time Polymerase Chain Reaction |
| title_short | Detection of Bovine Milk Adulteration in Non-novine Milk Using Real-Time Polymerase Chain Reaction |
| title_sort | detection of bovine milk adulteration in non novine milk using real time polymerase chain reaction |
| topic | non-bovine milk; real-time polymerase chain reaction; food adulteration; authenticity analysis |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-6-030.pdf |
| work_keys_str_mv | AT liweisunguodonglujiarongzhaozhongkaiyangjie detectionofbovinemilkadulterationinnonnovinemilkusingrealtimepolymerasechainreaction |