Plant growth-promoting rhizobacteria: selective screening and characterization of drought-tolerant bacteria isolated from drought-prone soils
Plant growth promoting rhizobacteria (PGPR) have evolved to thrive in challenging environments and provide the plants with defense against the harmful impacts of the environmental stressors. The present study aimed to isolate, screen, and identify PGPB obtained from the rhizosphere soil of some f...
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ResearchersLinks, Ltd
2024-06-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_361805_344042dbfad06c23547938ef7b18749a.pdf |
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| author | Osama M. Ghanem Nehal Kamal Alaa F. Said |
| author_facet | Osama M. Ghanem Nehal Kamal Alaa F. Said |
| author_sort | Osama M. Ghanem |
| collection | DOAJ |
| description | Plant growth promoting rhizobacteria (PGPR) have evolved to thrive in challenging
environments and provide the plants with defense against the harmful impacts of the
environmental stressors. The present study aimed to isolate, screen, and identify PGPB
obtained from the rhizosphere soil of some field crops at different sites in Ismailia, Egypt.
Isolation process was carried out from drought-suffered locations to obtain efficient promising
strains adapted to carry out vital processes under an irrigation water shortage. Eight bacterial
isolates were selected and identified by phenotypic properties, and were subjected to screening
procedures to assess their growth capabilities and evaluate their potential as PGPB. The
screening process involved investigating various PGPB features, such as phosphate
solubilization, formation of indole-3-acetic acid (IAA), ammonia, and hydrogen cyanide
(HCN) production. Among the eight isolates, two isolates only (MW3 and AB3) gave positive
results for all the tested plant growth promoting traits. The promising isolates were identified
by sequencing of their16S rRNA as Arthrobacter globiformis (MW3) and Micrococcus luteus
(AB3). A field trial was conducted to evaluate the activity of the two PGPB and their mixture
to act as biofertilizers for maize under deficit irrigation 0.75 from crop evapotranspiration
(ETc). All tested inoculants significantly increased yield components of maize, NPK uptake
by plants, availability of N and P in soil, activity of some soil enzymes, and total bacterial
counts compared to the un-inoculated control. Utilization of stress adapting PGPR showed
great potential in overcoming the challenges of sustainable agriculture under environmental
stress conditions. |
| format | Article |
| id | doaj-art-9c8b1dbd9ce34b7abe3dca5587eeb53d |
| institution | DOAJ |
| issn | 2537-0286 2537-0294 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | ResearchersLinks, Ltd |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj-art-9c8b1dbd9ce34b7abe3dca5587eeb53d2025-08-20T03:19:49ZengResearchersLinks, LtdNovel Research in Microbiology Journal2537-02862537-02942024-06-01832469249010.21608/NRMJ.2024.293974.1607Plant growth-promoting rhizobacteria: selective screening and characterization of drought-tolerant bacteria isolated from drought-prone soilsOsama M. Ghanem0Nehal Kamal1 Alaa F. Said2Soil and Water Department, Faculty of Agriculture, Suez Canal University, Ismailia 41522, EgyptDepartment of Botany and Microbiology, Faculty of Science, Suez University, Suez 43512, EgyptAgricultural Botany Department, Faculty of Agriculture, Suez Canal University, Ismailia 41522, EgyptPlant growth promoting rhizobacteria (PGPR) have evolved to thrive in challenging environments and provide the plants with defense against the harmful impacts of the environmental stressors. The present study aimed to isolate, screen, and identify PGPB obtained from the rhizosphere soil of some field crops at different sites in Ismailia, Egypt. Isolation process was carried out from drought-suffered locations to obtain efficient promising strains adapted to carry out vital processes under an irrigation water shortage. Eight bacterial isolates were selected and identified by phenotypic properties, and were subjected to screening procedures to assess their growth capabilities and evaluate their potential as PGPB. The screening process involved investigating various PGPB features, such as phosphate solubilization, formation of indole-3-acetic acid (IAA), ammonia, and hydrogen cyanide (HCN) production. Among the eight isolates, two isolates only (MW3 and AB3) gave positive results for all the tested plant growth promoting traits. The promising isolates were identified by sequencing of their16S rRNA as Arthrobacter globiformis (MW3) and Micrococcus luteus (AB3). A field trial was conducted to evaluate the activity of the two PGPB and their mixture to act as biofertilizers for maize under deficit irrigation 0.75 from crop evapotranspiration (ETc). All tested inoculants significantly increased yield components of maize, NPK uptake by plants, availability of N and P in soil, activity of some soil enzymes, and total bacterial counts compared to the un-inoculated control. Utilization of stress adapting PGPR showed great potential in overcoming the challenges of sustainable agriculture under environmental stress conditions.https://nrmj.journals.ekb.eg/article_361805_344042dbfad06c23547938ef7b18749a.pdfdrought stresspgpbmaizesoil enzymes16s rrna sequencing |
| spellingShingle | Osama M. Ghanem Nehal Kamal Alaa F. Said Plant growth-promoting rhizobacteria: selective screening and characterization of drought-tolerant bacteria isolated from drought-prone soils Novel Research in Microbiology Journal drought stress pgpb maize soil enzymes 16s rrna sequencing |
| title | Plant growth-promoting rhizobacteria: selective screening and characterization of drought-tolerant bacteria isolated from drought-prone soils |
| title_full | Plant growth-promoting rhizobacteria: selective screening and characterization of drought-tolerant bacteria isolated from drought-prone soils |
| title_fullStr | Plant growth-promoting rhizobacteria: selective screening and characterization of drought-tolerant bacteria isolated from drought-prone soils |
| title_full_unstemmed | Plant growth-promoting rhizobacteria: selective screening and characterization of drought-tolerant bacteria isolated from drought-prone soils |
| title_short | Plant growth-promoting rhizobacteria: selective screening and characterization of drought-tolerant bacteria isolated from drought-prone soils |
| title_sort | plant growth promoting rhizobacteria selective screening and characterization of drought tolerant bacteria isolated from drought prone soils |
| topic | drought stress pgpb maize soil enzymes 16s rrna sequencing |
| url | https://nrmj.journals.ekb.eg/article_361805_344042dbfad06c23547938ef7b18749a.pdf |
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