Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda
Abstract Objective This study presents the whole-genome sequence of Staphylococcus aureus strain MD02, isolated from a diabetic foot ulcer at a tertiary hospital in southwestern Uganda. The objective was to characterize the genome to understand the isolate’s resistance and virulence potential. Data...
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2025-07-01
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| Series: | BMC Genomic Data |
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| Online Access: | https://doi.org/10.1186/s12863-025-01342-1 |
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| author | Danladi Makeri Emmanuel Eilu Martin Odoki Ismail Abiola Adebayo Reuben Maghembe Musoba Abubakar Reagan Muhwezi Theophilus Pius Priscilla Peter Dilli Saheed Adekunle Akinola Ezera Agwu |
| author_facet | Danladi Makeri Emmanuel Eilu Martin Odoki Ismail Abiola Adebayo Reuben Maghembe Musoba Abubakar Reagan Muhwezi Theophilus Pius Priscilla Peter Dilli Saheed Adekunle Akinola Ezera Agwu |
| author_sort | Danladi Makeri |
| collection | DOAJ |
| description | Abstract Objective This study presents the whole-genome sequence of Staphylococcus aureus strain MD02, isolated from a diabetic foot ulcer at a tertiary hospital in southwestern Uganda. The objective was to characterize the genome to understand the isolate’s resistance and virulence potential. Data description : Genomic DNA of S. aureus strain MD02 was extracted and sequenced using the Illumina platform, generating high-quality paired-end reads with an average genome coverage of 32.6×. Quality control was performed with FastQC and reads were trimmed using Trimmomatic. De novo assembly was carried out using SPAdes, resulting in a draft genome of 2,815,980 bp assembled into 68 contigs with a GC content of 32.5%. Quality analysis of the genome revealed 98% completeness, and 2.48% contamination and the closest type strain to our isolate was Staphylococcus aureus (GCA_000330825.2) with an average nucleotide identity of 97.14%% and genome coverage of 89.38% confirming species level identity and close relatedness. Annotation using NCBI Prokaryotic Genome Annotation Pipeline identified 2,701 protein coding genes. |
| format | Article |
| id | doaj-art-9c78a3de426e4397a70716bcdabdd5f4 |
| institution | DOAJ |
| issn | 2730-6844 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomic Data |
| spelling | doaj-art-9c78a3de426e4397a70716bcdabdd5f42025-08-20T03:06:09ZengBMCBMC Genomic Data2730-68442025-07-012611310.1186/s12863-025-01342-1Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in UgandaDanladi Makeri0Emmanuel Eilu1Martin Odoki2Ismail Abiola Adebayo3Reuben Maghembe4Musoba Abubakar5Reagan Muhwezi6Theophilus Pius7Priscilla Peter Dilli8Saheed Adekunle Akinola9Ezera Agwu10Department of Microbiology and Immunology, Kampala International University Western CampusDepartment of Microbiology and Immunology, School of Medicine, King Ceasor UniversityDepartment of Applied Sciences, School of Sciences, Nkumba UniversityDepartment of Microbiology, Parasitology, and Immunology, School of Medicine, Kabale UniversityDepartment of Immunology and Molecular Biology, College of Health Sciences, Makerere UniversityInstitute of Biomedical Research, Kampala International University Western CampusDepartment of Microbiology and Immunology, Kampala International University Western CampusDepartment of Medical Laboratory Science, Kampala International University Western CampusDepartment of Public Health, Kampala International UniversityDepartment of Microbiology and Parasitology, School of Medicine and Pharmacy, College of Medicine and Health Sciences, University of RwandaDepartment of Microbiology and Immunology, Kampala International University Western CampusAbstract Objective This study presents the whole-genome sequence of Staphylococcus aureus strain MD02, isolated from a diabetic foot ulcer at a tertiary hospital in southwestern Uganda. The objective was to characterize the genome to understand the isolate’s resistance and virulence potential. Data description : Genomic DNA of S. aureus strain MD02 was extracted and sequenced using the Illumina platform, generating high-quality paired-end reads with an average genome coverage of 32.6×. Quality control was performed with FastQC and reads were trimmed using Trimmomatic. De novo assembly was carried out using SPAdes, resulting in a draft genome of 2,815,980 bp assembled into 68 contigs with a GC content of 32.5%. Quality analysis of the genome revealed 98% completeness, and 2.48% contamination and the closest type strain to our isolate was Staphylococcus aureus (GCA_000330825.2) with an average nucleotide identity of 97.14%% and genome coverage of 89.38% confirming species level identity and close relatedness. Annotation using NCBI Prokaryotic Genome Annotation Pipeline identified 2,701 protein coding genes.https://doi.org/10.1186/s12863-025-01342-1Wound bacteriumDiabetic foot ulcerStaphylococcus aureusWhole-genome sequencingComplete genome sequence of S. aureus |
| spellingShingle | Danladi Makeri Emmanuel Eilu Martin Odoki Ismail Abiola Adebayo Reuben Maghembe Musoba Abubakar Reagan Muhwezi Theophilus Pius Priscilla Peter Dilli Saheed Adekunle Akinola Ezera Agwu Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda BMC Genomic Data Wound bacterium Diabetic foot ulcer Staphylococcus aureus Whole-genome sequencing Complete genome sequence of S. aureus |
| title | Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda |
| title_full | Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda |
| title_fullStr | Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda |
| title_full_unstemmed | Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda |
| title_short | Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda |
| title_sort | whole genome sequence of a virulent and multidrug resistant staphylococcus aureus strain md02 isolated from a diabetic foot ulcer in uganda |
| topic | Wound bacterium Diabetic foot ulcer Staphylococcus aureus Whole-genome sequencing Complete genome sequence of S. aureus |
| url | https://doi.org/10.1186/s12863-025-01342-1 |
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