A tutorial on the synthesis and applications of molecularly imprinted polymers in analytical chemistry

Molecularly imprinted polymers (MIPs) have emerged as highly selective sorbents with tailor-made recognition sites complementary to target analytes in terms of shape, size, and functional groups. This tutorial provides a comprehensive overview of the principles, synthesis strategies, and analytical...

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Main Authors: N.P. Kalogiouri, A. Kabir, K.G. Furton, V.F. Samanidou
Format: Article
Language:English
Published: Elsevier 2025-11-01
Series:Journal of Chromatography Open
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2772391725000428
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author N.P. Kalogiouri
A. Kabir
K.G. Furton
V.F. Samanidou
author_facet N.P. Kalogiouri
A. Kabir
K.G. Furton
V.F. Samanidou
author_sort N.P. Kalogiouri
collection DOAJ
description Molecularly imprinted polymers (MIPs) have emerged as highly selective sorbents with tailor-made recognition sites complementary to target analytes in terms of shape, size, and functional groups. This tutorial provides a comprehensive overview of the principles, synthesis strategies, and analytical applications of MIPs, with a focus on their utility in sample preparation, separation, and detection. Various imprinting techniques—such as bulk, surface, and nanoimprinting—are discussed alongside advances in green synthesis and novel monomer-template systems. Emphasis is placed on the integration of MIPs with chromatographic and spectrometric platforms, particularly in complex matrices relevant to environmental, pharmaceutical, food, and clinical analysis. The tutorial illustrates the practical considerations for designing and optimizing MIP-based methods, including template selection, polymerization conditions, and reusability, reflecting recent and relevant advances of the last ten years This tutorial aims to guide researchers and analysts in leveraging the unique properties of MIPs to enhance selectivity, sensitivity, and sustainability in modern analytical chemistry.
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spelling doaj-art-9c74cdc3240a42ca99143034b86abe722025-08-20T03:33:07ZengElsevierJournal of Chromatography Open2772-39172025-11-01810024410.1016/j.jcoa.2025.100244A tutorial on the synthesis and applications of molecularly imprinted polymers in analytical chemistryN.P. Kalogiouri0A. Kabir1K.G. Furton2V.F. Samanidou3Laboratory of Analytical Chemistry, School of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, GR-54124, GreeceGlobal Forensic and Justice Center, Department of Chemistry and Biochemistry, Florida International University, Miami, USAGlobal Forensic and Justice Center, Department of Chemistry and Biochemistry, Florida International University, Miami, USALaboratory of Analytical Chemistry, School of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, GR-54124, Greece; Corresponding author.Molecularly imprinted polymers (MIPs) have emerged as highly selective sorbents with tailor-made recognition sites complementary to target analytes in terms of shape, size, and functional groups. This tutorial provides a comprehensive overview of the principles, synthesis strategies, and analytical applications of MIPs, with a focus on their utility in sample preparation, separation, and detection. Various imprinting techniques—such as bulk, surface, and nanoimprinting—are discussed alongside advances in green synthesis and novel monomer-template systems. Emphasis is placed on the integration of MIPs with chromatographic and spectrometric platforms, particularly in complex matrices relevant to environmental, pharmaceutical, food, and clinical analysis. The tutorial illustrates the practical considerations for designing and optimizing MIP-based methods, including template selection, polymerization conditions, and reusability, reflecting recent and relevant advances of the last ten years This tutorial aims to guide researchers and analysts in leveraging the unique properties of MIPs to enhance selectivity, sensitivity, and sustainability in modern analytical chemistry.http://www.sciencedirect.com/science/article/pii/S2772391725000428Engineered selectivityMolecularly imprinted polymerRecognition materialsSynthetic antibody
spellingShingle N.P. Kalogiouri
A. Kabir
K.G. Furton
V.F. Samanidou
A tutorial on the synthesis and applications of molecularly imprinted polymers in analytical chemistry
Journal of Chromatography Open
Engineered selectivity
Molecularly imprinted polymer
Recognition materials
Synthetic antibody
title A tutorial on the synthesis and applications of molecularly imprinted polymers in analytical chemistry
title_full A tutorial on the synthesis and applications of molecularly imprinted polymers in analytical chemistry
title_fullStr A tutorial on the synthesis and applications of molecularly imprinted polymers in analytical chemistry
title_full_unstemmed A tutorial on the synthesis and applications of molecularly imprinted polymers in analytical chemistry
title_short A tutorial on the synthesis and applications of molecularly imprinted polymers in analytical chemistry
title_sort tutorial on the synthesis and applications of molecularly imprinted polymers in analytical chemistry
topic Engineered selectivity
Molecularly imprinted polymer
Recognition materials
Synthetic antibody
url http://www.sciencedirect.com/science/article/pii/S2772391725000428
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