Phylogenetic insights into the genus Scytonema Bornet et Flahault (Cyanobacteria): A 16S rRNA gene review with the taxonomic assessment of S. schmidtii Gomont
Abstract This study aims to reevaluate the diversity of 16S rRNA gene sequences associated with Scytonema (Nostocales, Cyanobacteria). For that, we first conducted a search for ‘Scytonema’ sequences available in GenBank (NCBI) and aligned the results with reference strains from other Nostocales gene...
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Sociedade Botânica do Brasil
2025-05-01
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| Series: | Acta Botânica Brasílica |
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| author | Guilherme Scotta Hentschke Célia Leite Sant’Anna Vitor Vasconcelos |
| author_facet | Guilherme Scotta Hentschke Célia Leite Sant’Anna Vitor Vasconcelos |
| author_sort | Guilherme Scotta Hentschke |
| collection | DOAJ |
| description | Abstract This study aims to reevaluate the diversity of 16S rRNA gene sequences associated with Scytonema (Nostocales, Cyanobacteria). For that, we first conducted a search for ‘Scytonema’ sequences available in GenBank (NCBI) and aligned the results with reference strains from other Nostocales genera. The alignment totalized 383 OTUs and 800 nucleotide positions analyzed. A FastTree phylogenetic analysis revealed that the ‘Scytonema’ sequences are distributed across 15 distinct clades in addition to the Scytonema sensu stricto clade. These additional clades are positioned among Nostocales genera and phylogenetically distant from the type species, S. hofmanni. Within these clades, we had access to the strain Scytonema sp. CCIBt3568, identified as S. schmidtii, which we believe should be separated from Scytonema, and described as a new genus in the future. Additionally, Bayesian Inference and Maximum Likelihood phylogenies were performed with 97 OTUs and 971 bp, and the polyphyletic status of Scytonema as currently recognized was confirmed. |
| format | Article |
| id | doaj-art-9c6dddad9e1145058497a316c0ff7e99 |
| institution | DOAJ |
| issn | 1677-941X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Sociedade Botânica do Brasil |
| record_format | Article |
| series | Acta Botânica Brasílica |
| spelling | doaj-art-9c6dddad9e1145058497a316c0ff7e992025-08-20T03:07:32ZengSociedade Botânica do BrasilActa Botânica Brasílica1677-941X2025-05-013910.1590/1677-941x-abb-2024-0044Phylogenetic insights into the genus Scytonema Bornet et Flahault (Cyanobacteria): A 16S rRNA gene review with the taxonomic assessment of S. schmidtii GomontGuilherme Scotta Hentschkehttps://orcid.org/0000-0003-4396-024XCélia Leite Sant’Annahttps://orcid.org/0000-0001-7706-756XVitor Vasconceloshttps://orcid.org/0000-0003-3585-2417Abstract This study aims to reevaluate the diversity of 16S rRNA gene sequences associated with Scytonema (Nostocales, Cyanobacteria). For that, we first conducted a search for ‘Scytonema’ sequences available in GenBank (NCBI) and aligned the results with reference strains from other Nostocales genera. The alignment totalized 383 OTUs and 800 nucleotide positions analyzed. A FastTree phylogenetic analysis revealed that the ‘Scytonema’ sequences are distributed across 15 distinct clades in addition to the Scytonema sensu stricto clade. These additional clades are positioned among Nostocales genera and phylogenetically distant from the type species, S. hofmanni. Within these clades, we had access to the strain Scytonema sp. CCIBt3568, identified as S. schmidtii, which we believe should be separated from Scytonema, and described as a new genus in the future. Additionally, Bayesian Inference and Maximum Likelihood phylogenies were performed with 97 OTUs and 971 bp, and the polyphyletic status of Scytonema as currently recognized was confirmed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062025000100110&lng=en&tlng=enbiodiversityNostocalespolyphasic approachScytonemataxonomical reviewtropical environments |
| spellingShingle | Guilherme Scotta Hentschke Célia Leite Sant’Anna Vitor Vasconcelos Phylogenetic insights into the genus Scytonema Bornet et Flahault (Cyanobacteria): A 16S rRNA gene review with the taxonomic assessment of S. schmidtii Gomont Acta Botânica Brasílica biodiversity Nostocales polyphasic approach Scytonema taxonomical review tropical environments |
| title | Phylogenetic insights into the genus Scytonema Bornet et Flahault (Cyanobacteria): A 16S rRNA gene review with the taxonomic assessment of S. schmidtii Gomont |
| title_full | Phylogenetic insights into the genus Scytonema Bornet et Flahault (Cyanobacteria): A 16S rRNA gene review with the taxonomic assessment of S. schmidtii Gomont |
| title_fullStr | Phylogenetic insights into the genus Scytonema Bornet et Flahault (Cyanobacteria): A 16S rRNA gene review with the taxonomic assessment of S. schmidtii Gomont |
| title_full_unstemmed | Phylogenetic insights into the genus Scytonema Bornet et Flahault (Cyanobacteria): A 16S rRNA gene review with the taxonomic assessment of S. schmidtii Gomont |
| title_short | Phylogenetic insights into the genus Scytonema Bornet et Flahault (Cyanobacteria): A 16S rRNA gene review with the taxonomic assessment of S. schmidtii Gomont |
| title_sort | phylogenetic insights into the genus scytonema bornet et flahault cyanobacteria a 16s rrna gene review with the taxonomic assessment of s schmidtii gomont |
| topic | biodiversity Nostocales polyphasic approach Scytonema taxonomical review tropical environments |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062025000100110&lng=en&tlng=en |
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