Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients
Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infections acquired in both community and hospital settings. In this study, MRSA isolated from different sources of hospitalized patients was characterized by molecular and phenotypic methods. Methodolo...
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The Journal of Infection in Developing Countries
2015-07-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/5868 |
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| author | Caio Ferreira de Oliveira Alexandre Tadachi Morey Jussevania Pereira Santos Ludmila Vilela Pereira Gomes Juscélio Donizete Cardoso Phileno Pinge-Filho Márcia Regina Eches Perugini Lucy Megumi Yamauchi Sueli Fumie Yamada-Ogattta |
| author_facet | Caio Ferreira de Oliveira Alexandre Tadachi Morey Jussevania Pereira Santos Ludmila Vilela Pereira Gomes Juscélio Donizete Cardoso Phileno Pinge-Filho Márcia Regina Eches Perugini Lucy Megumi Yamauchi Sueli Fumie Yamada-Ogattta |
| author_sort | Caio Ferreira de Oliveira |
| collection | DOAJ |
| description | Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infections acquired in both community and hospital settings. In this study, MRSA isolated from different sources of hospitalized patients was characterized by molecular and phenotypic methods.
Methodology: A total of 123 S. aureus isolates were characterized according to their genetic relatedness by repetitive element sequence based-PCR (REP-PCR), in vitro antimicrobial susceptibility profile, SCCmec typing and presence of seven virulence factor-encoding genes.
Results: REP-PCR fingerprinting showed low relatedness between the isolates, and the predominance of one specific lineage or clonal group was not observed. All isolates were susceptible to teicoplanin and linezolide. All isolates were resistant to cefoxitin and penicillin, and the majority were also resistant to one or more other antimicrobials. Fifty isolates (41.7%) were intermediately resistant to vancomycin. Most isolates harbored SCCmec type II (53.7%), followed by type I (22.8%), type IV (8.1%) and type III (1.6%). All isolates harbored at least two virulence factor-encoding genes, and the prevalence was as follows: coa, 100%; icaA, 100%; hla, 13.0%; hlb, 91.1%, hld, 91.1%; lukS-PV and lukF-PV, 2.4%; and tst, 34.1%. A positive association with the presence of hla and SCCmec type II, and tst and SCCmec type I was observed.
Conclusion: This study showed the high virulence potential of multidrug-resistant MRSA circulating in a teaching hospital. A high prevalence of MRSA showing intermediate vancomycin resistance was also observed, indicating the urgent need to improve strategies for controlling the use of antimicrobials for appropriate management of S. aureus infections.
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| format | Article |
| id | doaj-art-9c5d648484cc42989820dc9214bf5a9f |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-9c5d648484cc42989820dc9214bf5a9f2025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-07-0190710.3855/jidc.5868Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patientsCaio Ferreira de Oliveira0Alexandre Tadachi Morey1Jussevania Pereira Santos2Ludmila Vilela Pereira Gomes3Juscélio Donizete Cardoso4Phileno Pinge-Filho5Márcia Regina Eches Perugini6Lucy Megumi Yamauchi7Sueli Fumie Yamada-Ogattta8Centro de Ciências Biológicas, Universidade Estadual de Londrina. Londrina, Paraná, BrazilCentro de Ciências Biológicas, Universidade Estadual de Londrina. Londrina, Paraná, BrazilCentro de Ciências Biológicas, Universidade Estadual de Londrina. Londrina, Paraná, BrazilCentro de Ciências Biológicas, Universidade Estadual de Londrina. Londrina, Paraná, BrazilInstituto Agronômico do Paraná, Departamento de Microbiologia do Solo, Londrina, Paraná, BrazilCentro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná, BrazilCentro de Ciências da Saúde, Universidade Estadual de Londrina, Londrina, Paraná, BrazilCentro de Ciências da Saúde, Universidade Estadual de Londrina, Londrina, Paraná, BrazilCentro de Ciências Biológicas, Universidade Estadual de Londrina. Londrina, Paraná, BrazilIntroduction: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infections acquired in both community and hospital settings. In this study, MRSA isolated from different sources of hospitalized patients was characterized by molecular and phenotypic methods. Methodology: A total of 123 S. aureus isolates were characterized according to their genetic relatedness by repetitive element sequence based-PCR (REP-PCR), in vitro antimicrobial susceptibility profile, SCCmec typing and presence of seven virulence factor-encoding genes. Results: REP-PCR fingerprinting showed low relatedness between the isolates, and the predominance of one specific lineage or clonal group was not observed. All isolates were susceptible to teicoplanin and linezolide. All isolates were resistant to cefoxitin and penicillin, and the majority were also resistant to one or more other antimicrobials. Fifty isolates (41.7%) were intermediately resistant to vancomycin. Most isolates harbored SCCmec type II (53.7%), followed by type I (22.8%), type IV (8.1%) and type III (1.6%). All isolates harbored at least two virulence factor-encoding genes, and the prevalence was as follows: coa, 100%; icaA, 100%; hla, 13.0%; hlb, 91.1%, hld, 91.1%; lukS-PV and lukF-PV, 2.4%; and tst, 34.1%. A positive association with the presence of hla and SCCmec type II, and tst and SCCmec type I was observed. Conclusion: This study showed the high virulence potential of multidrug-resistant MRSA circulating in a teaching hospital. A high prevalence of MRSA showing intermediate vancomycin resistance was also observed, indicating the urgent need to improve strategies for controlling the use of antimicrobials for appropriate management of S. aureus infections. https://jidc.org/index.php/journal/article/view/5868antimicrobial resistanceintermediate vancomycin resistanceMRSASCCmec typingvirulence factors |
| spellingShingle | Caio Ferreira de Oliveira Alexandre Tadachi Morey Jussevania Pereira Santos Ludmila Vilela Pereira Gomes Juscélio Donizete Cardoso Phileno Pinge-Filho Márcia Regina Eches Perugini Lucy Megumi Yamauchi Sueli Fumie Yamada-Ogattta Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients Journal of Infection in Developing Countries antimicrobial resistance intermediate vancomycin resistance MRSA SCCmec typing virulence factors |
| title | Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients |
| title_full | Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients |
| title_fullStr | Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients |
| title_full_unstemmed | Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients |
| title_short | Molecular and phenotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from hospitalized patients |
| title_sort | molecular and phenotypic characteristics of methicillin resistant staphylococcus aureus isolated from hospitalized patients |
| topic | antimicrobial resistance intermediate vancomycin resistance MRSA SCCmec typing virulence factors |
| url | https://jidc.org/index.php/journal/article/view/5868 |
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