Structure of a Spumaretrovirus Gag Central Domain Reveals an Ancient Retroviral Capsid.
The Spumaretrovirinae, or foamy viruses (FVs) are complex retroviruses that infect many species of monkey and ape. Despite little sequence homology, FV and orthoretroviral Gag proteins perform equivalent functions, including genome packaging, virion assembly, trafficking and membrane targeting. Howe...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-11-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005981&type=printable |
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| author | Neil J Ball Giuseppe Nicastro Moumita Dutta Dominic J Pollard David C Goldstone Marta Sanz-Ramos Andres Ramos Erik Müllers Kristin Stirnnagel Nicole Stanke Dirk Lindemann Jonathan P Stoye William R Taylor Peter B Rosenthal Ian A Taylor |
| author_facet | Neil J Ball Giuseppe Nicastro Moumita Dutta Dominic J Pollard David C Goldstone Marta Sanz-Ramos Andres Ramos Erik Müllers Kristin Stirnnagel Nicole Stanke Dirk Lindemann Jonathan P Stoye William R Taylor Peter B Rosenthal Ian A Taylor |
| author_sort | Neil J Ball |
| collection | DOAJ |
| description | The Spumaretrovirinae, or foamy viruses (FVs) are complex retroviruses that infect many species of monkey and ape. Despite little sequence homology, FV and orthoretroviral Gag proteins perform equivalent functions, including genome packaging, virion assembly, trafficking and membrane targeting. However, there is a paucity of structural information for FVs and it is unclear how disparate FV and orthoretroviral Gag molecules share the same function. To probe the functional overlap of FV and orthoretroviral Gag we have determined the structure of a central region of Gag from the Prototype FV (PFV). The structure comprises two all α-helical domains NtDCEN and CtDCEN that although they have no sequence similarity, we show they share the same core fold as the N- (NtDCA) and C-terminal domains (CtDCA) of archetypal orthoretroviral capsid protein (CA). Moreover, structural comparisons with orthoretroviral CA align PFV NtDCEN and CtDCEN with NtDCA and CtDCA respectively. Further in vitro and functional virological assays reveal that residues making inter-domain NtDCEN-CtDCEN interactions are required for PFV capsid assembly and that intact capsid is required for PFV reverse transcription. These data provide the first information that relates the Gag proteins of Spuma and Orthoretrovirinae and suggests a common ancestor for both lineages containing an ancient CA fold. |
| format | Article |
| id | doaj-art-9c57cb193dd74b4fa16644edfab5318c |
| institution | Kabale University |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2016-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-9c57cb193dd74b4fa16644edfab5318c2025-08-20T03:26:16ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742016-11-011211e100598110.1371/journal.ppat.1005981Structure of a Spumaretrovirus Gag Central Domain Reveals an Ancient Retroviral Capsid.Neil J BallGiuseppe NicastroMoumita DuttaDominic J PollardDavid C GoldstoneMarta Sanz-RamosAndres RamosErik MüllersKristin StirnnagelNicole StankeDirk LindemannJonathan P StoyeWilliam R TaylorPeter B RosenthalIan A TaylorThe Spumaretrovirinae, or foamy viruses (FVs) are complex retroviruses that infect many species of monkey and ape. Despite little sequence homology, FV and orthoretroviral Gag proteins perform equivalent functions, including genome packaging, virion assembly, trafficking and membrane targeting. However, there is a paucity of structural information for FVs and it is unclear how disparate FV and orthoretroviral Gag molecules share the same function. To probe the functional overlap of FV and orthoretroviral Gag we have determined the structure of a central region of Gag from the Prototype FV (PFV). The structure comprises two all α-helical domains NtDCEN and CtDCEN that although they have no sequence similarity, we show they share the same core fold as the N- (NtDCA) and C-terminal domains (CtDCA) of archetypal orthoretroviral capsid protein (CA). Moreover, structural comparisons with orthoretroviral CA align PFV NtDCEN and CtDCEN with NtDCA and CtDCA respectively. Further in vitro and functional virological assays reveal that residues making inter-domain NtDCEN-CtDCEN interactions are required for PFV capsid assembly and that intact capsid is required for PFV reverse transcription. These data provide the first information that relates the Gag proteins of Spuma and Orthoretrovirinae and suggests a common ancestor for both lineages containing an ancient CA fold.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005981&type=printable |
| spellingShingle | Neil J Ball Giuseppe Nicastro Moumita Dutta Dominic J Pollard David C Goldstone Marta Sanz-Ramos Andres Ramos Erik Müllers Kristin Stirnnagel Nicole Stanke Dirk Lindemann Jonathan P Stoye William R Taylor Peter B Rosenthal Ian A Taylor Structure of a Spumaretrovirus Gag Central Domain Reveals an Ancient Retroviral Capsid. PLoS Pathogens |
| title | Structure of a Spumaretrovirus Gag Central Domain Reveals an Ancient Retroviral Capsid. |
| title_full | Structure of a Spumaretrovirus Gag Central Domain Reveals an Ancient Retroviral Capsid. |
| title_fullStr | Structure of a Spumaretrovirus Gag Central Domain Reveals an Ancient Retroviral Capsid. |
| title_full_unstemmed | Structure of a Spumaretrovirus Gag Central Domain Reveals an Ancient Retroviral Capsid. |
| title_short | Structure of a Spumaretrovirus Gag Central Domain Reveals an Ancient Retroviral Capsid. |
| title_sort | structure of a spumaretrovirus gag central domain reveals an ancient retroviral capsid |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005981&type=printable |
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