Enrichment of Artemia sp. with autochthonous probiotics at different levels in larviculture of piauçu Megaleporinus macrocephalus
The research investigated the effect of dietary supplementation with Artemia sp. enriched with the autochthonous probiotic Enterococcus faecium on growth performance, microbiota modulation, intestinal morphology, and resistance to pathogenic bacteria of Megaleporinus macrocephalus larvae. The study...
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Instituto de Pesca
2023-09-01
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| Series: | Boletim do Instituto de Pesca |
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| Online Access: | https://institutodepesca.org/index.php/bip/article/view/1852 |
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| author | Francisco Alex Lima Barros Joel Artur Rodrigues Dias Higo Andrade Abe Keber Santos Costa Júnior Natalino da Costa Sousa Carlos Alberto Martins Cordeiro Rodrigo Yudi Fujimoto |
| author_facet | Francisco Alex Lima Barros Joel Artur Rodrigues Dias Higo Andrade Abe Keber Santos Costa Júnior Natalino da Costa Sousa Carlos Alberto Martins Cordeiro Rodrigo Yudi Fujimoto |
| author_sort | Francisco Alex Lima Barros |
| collection | DOAJ |
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The research investigated the effect of dietary supplementation with Artemia sp. enriched with the autochthonous probiotic Enterococcus faecium on growth performance, microbiota modulation, intestinal morphology, and resistance to pathogenic bacteria of Megaleporinus macrocephalus larvae. The study evaluated four treatments (C: without probiotics; T1:1 × 104; T2:1× 106; and T3:1 × 108 CFU·mL-1) in quadruplicates. The larvae (n=160; weight= 5.3 ± 2.3mg and length= 3.73±0.4mm) were distributed in 16 L containers at a density of 10 larvae·L-1 for 20 days. The productive performance, survival, gut microbiology, and histology were measured. The larvae were also submitted to acute challenge against the pathogenic bacterium Aeromonas hydrophila. The results showed that supplementation with 1 × 108 CFU·mL-1 promotes greater gain in length (13.78 ± 0.40 cm) and total weight (0.08 ± 0.002 g), higher counts of lactic acid bacteria and lower total heterotrophic in the intestines (7.11±0.30; 0.12 ± 0.09 log CFU·g-1, respectively) and larger villi (0.26 ± 0.03μm). Diets containing probiotics influenced the animals’ resistance to acute infection, with a lower accumulated mortality in T3 (33% ± 11.54%) and a higher one in C+ (93% ± 11.54%). Thus, probiotic supplementation with the autochthonous bacterium E. faecium (1×108CFU·mL-1) provides zootechnical improvement, villus increase and greater resistance to infections.
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| format | Article |
| id | doaj-art-9c48dc80a2dd4b87ac7afc227efa0cd8 |
| institution | DOAJ |
| issn | 1678-2305 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Instituto de Pesca |
| record_format | Article |
| series | Boletim do Instituto de Pesca |
| spelling | doaj-art-9c48dc80a2dd4b87ac7afc227efa0cd82025-08-20T02:56:39ZengInstituto de PescaBoletim do Instituto de Pesca1678-23052023-09-0149Enrichment of Artemia sp. with autochthonous probiotics at different levels in larviculture of piauçu Megaleporinus macrocephalusFrancisco Alex Lima Barros0Joel Artur Rodrigues Dias1Higo Andrade Abe2Keber Santos Costa Júnior3Natalino da Costa Sousa4Carlos Alberto Martins Cordeiro5Rodrigo Yudi Fujimoto6Instituto Federal de Educação, Ciência e Tecnologia do Pará – Cametá (PA), Brazil.Universidade Federal do Maranhão – Pinheiro (MA), Brazil.Universidade Federal do Amazonas – Manaus (AM), Brazil.Universidade Federal do Pará – Bragança (PA), Brazil.Universidade Federal do Pará – Bragança (PA), Brazil.Universidade Federal do Pará – Bragança (PA), Brazil.Empresa Brasileira de Pesquisa Agropecuária – Aracaju (SE), Brazil. The research investigated the effect of dietary supplementation with Artemia sp. enriched with the autochthonous probiotic Enterococcus faecium on growth performance, microbiota modulation, intestinal morphology, and resistance to pathogenic bacteria of Megaleporinus macrocephalus larvae. The study evaluated four treatments (C: without probiotics; T1:1 × 104; T2:1× 106; and T3:1 × 108 CFU·mL-1) in quadruplicates. The larvae (n=160; weight= 5.3 ± 2.3mg and length= 3.73±0.4mm) were distributed in 16 L containers at a density of 10 larvae·L-1 for 20 days. The productive performance, survival, gut microbiology, and histology were measured. The larvae were also submitted to acute challenge against the pathogenic bacterium Aeromonas hydrophila. The results showed that supplementation with 1 × 108 CFU·mL-1 promotes greater gain in length (13.78 ± 0.40 cm) and total weight (0.08 ± 0.002 g), higher counts of lactic acid bacteria and lower total heterotrophic in the intestines (7.11±0.30; 0.12 ± 0.09 log CFU·g-1, respectively) and larger villi (0.26 ± 0.03μm). Diets containing probiotics influenced the animals’ resistance to acute infection, with a lower accumulated mortality in T3 (33% ± 11.54%) and a higher one in C+ (93% ± 11.54%). Thus, probiotic supplementation with the autochthonous bacterium E. faecium (1×108CFU·mL-1) provides zootechnical improvement, villus increase and greater resistance to infections. https://institutodepesca.org/index.php/bip/article/view/1852Growth performanceLive foodSanityMicrobiologyPathogen |
| spellingShingle | Francisco Alex Lima Barros Joel Artur Rodrigues Dias Higo Andrade Abe Keber Santos Costa Júnior Natalino da Costa Sousa Carlos Alberto Martins Cordeiro Rodrigo Yudi Fujimoto Enrichment of Artemia sp. with autochthonous probiotics at different levels in larviculture of piauçu Megaleporinus macrocephalus Boletim do Instituto de Pesca Growth performance Live food Sanity Microbiology Pathogen |
| title | Enrichment of Artemia sp. with autochthonous probiotics at different levels in larviculture of piauçu Megaleporinus macrocephalus |
| title_full | Enrichment of Artemia sp. with autochthonous probiotics at different levels in larviculture of piauçu Megaleporinus macrocephalus |
| title_fullStr | Enrichment of Artemia sp. with autochthonous probiotics at different levels in larviculture of piauçu Megaleporinus macrocephalus |
| title_full_unstemmed | Enrichment of Artemia sp. with autochthonous probiotics at different levels in larviculture of piauçu Megaleporinus macrocephalus |
| title_short | Enrichment of Artemia sp. with autochthonous probiotics at different levels in larviculture of piauçu Megaleporinus macrocephalus |
| title_sort | enrichment of artemia sp with autochthonous probiotics at different levels in larviculture of piaucu megaleporinus macrocephalus |
| topic | Growth performance Live food Sanity Microbiology Pathogen |
| url | https://institutodepesca.org/index.php/bip/article/view/1852 |
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