Protocol for genetic dissection of class switch recombination using genome-editing tools

Protocol for genetic dissection of class switch recombination using genome-editing tools

Summary: Antibody class switch recombination is achieved through programmed DNA damage, and the processing of programmed DNA lesions requires the coordinated action of many DNA metabolic factors. Here, we present a protocol for inducing class switch recombination using base editors or CRISPR-Cas9. W...

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Bibliographic Details
Main Authors: Pengfei Dai, Tingting Liu, Dingpeng Yang, Yifeng Luo, Liu Daisy Liu, Xia Xie, Yafang Shang, Xiaojing Liu, Fei-Long Meng
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
cell-based assays
immunology
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002886
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