Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic Conditions
The antitumor properties of melatonin (MLT) are known for prostate cancer cells. This study investigated whether MLT affects prostate maturation and interferes with tissue injuries induced by diabetes. MLT was administered to Wistar rats from 5 weeks of age in the drinking water (10 μg/kg b.w.), and...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2015/538529 |
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| author | Marina G. Gobbo Nishtman Dizeyi Per-Anders Abrahamsson Per-Anders Bertilsson Viviane Sanches Masitéli Eloisa Zanin Pytlowanciv Sebastião R. Taboga Rejane M. Góes |
| author_facet | Marina G. Gobbo Nishtman Dizeyi Per-Anders Abrahamsson Per-Anders Bertilsson Viviane Sanches Masitéli Eloisa Zanin Pytlowanciv Sebastião R. Taboga Rejane M. Góes |
| author_sort | Marina G. Gobbo |
| collection | DOAJ |
| description | The antitumor properties of melatonin (MLT) are known for prostate cancer cells. This study investigated whether MLT affects prostate maturation and interferes with tissue injuries induced by diabetes. MLT was administered to Wistar rats from 5 weeks of age in the drinking water (10 μg/kg b.w.), and diabetes was induced at the 13th week by streptozotocin (4.5 mg/100g b.w., i.p.). The animals were euthanized in the 14th and 21st weeks. MLT reduced the immunostained cells for androgen receptor (AR) by 10% in younger rats. Diabetes decreased cell proliferation and increased apoptosis. MLT treatment impeded apoptosis p=0.02 and augmented proliferation p=0.0008 and PCNA content in prostate following long-term diabetes due to restoration of testosterone levels and expression of melatonin receptor type 1B. The effect of MLT (500 µM, 5 mM, and 10 mM) on androgen-dependent (22Rv1) and androgen-independent (PC3) cancer cells and human prostate epithelial cells (PNTA1) under normal and hyperglycemic conditions (HG, 450 mg/dL) was analyzed. Contrary to PNTA1 and 22Rv1 cells, MLT improved the proliferation of PC3 cells in hyperglycemic medium. The combined data indicated that MLT had proliferative and antiapoptotic effects in prostate cells subjected to HG levels and it seems to involve specific MLT pathways rather than AR. |
| format | Article |
| id | doaj-art-9c1bd47c167d4aa688f75b448673e533 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-9c1bd47c167d4aa688f75b448673e5332025-02-03T06:08:20ZengWileyJournal of Diabetes Research2314-67452314-67532015-01-01201510.1155/2015/538529538529Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic ConditionsMarina G. Gobbo0Nishtman Dizeyi1Per-Anders Abrahamsson2Per-Anders Bertilsson3Viviane Sanches Masitéli4Eloisa Zanin Pytlowanciv5Sebastião R. Taboga6Rejane M. Góes7Department of Cell Biology, Institute of Biology, UNICAMP, Avenue Bertrand Russel, 6109 Campinas, SP, BrazilDepartment of Clinical Sciences, Division of Urological Research, Skåne University Hospital, Lund University, 205 02 Malmö, SwedenDepartment of Clinical Sciences, Division of Urological Research, Skåne University Hospital, Lund University, 205 02 Malmö, SwedenDepartment of Clinical Sciences, Division of Urological Research, Skåne University Hospital, Lund University, 205 02 Malmö, SwedenDepartment of Biology, Institute of Biosciences, Humanities and Exact Sciences, UNESP, São José do Rio Preto, SP, BrazilDepartment of Cell Biology, Institute of Biology, UNICAMP, Avenue Bertrand Russel, 6109 Campinas, SP, BrazilDepartment of Biology, Institute of Biosciences, Humanities and Exact Sciences, UNESP, São José do Rio Preto, SP, BrazilDepartment of Biology, Institute of Biosciences, Humanities and Exact Sciences, UNESP, São José do Rio Preto, SP, BrazilThe antitumor properties of melatonin (MLT) are known for prostate cancer cells. This study investigated whether MLT affects prostate maturation and interferes with tissue injuries induced by diabetes. MLT was administered to Wistar rats from 5 weeks of age in the drinking water (10 μg/kg b.w.), and diabetes was induced at the 13th week by streptozotocin (4.5 mg/100g b.w., i.p.). The animals were euthanized in the 14th and 21st weeks. MLT reduced the immunostained cells for androgen receptor (AR) by 10% in younger rats. Diabetes decreased cell proliferation and increased apoptosis. MLT treatment impeded apoptosis p=0.02 and augmented proliferation p=0.0008 and PCNA content in prostate following long-term diabetes due to restoration of testosterone levels and expression of melatonin receptor type 1B. The effect of MLT (500 µM, 5 mM, and 10 mM) on androgen-dependent (22Rv1) and androgen-independent (PC3) cancer cells and human prostate epithelial cells (PNTA1) under normal and hyperglycemic conditions (HG, 450 mg/dL) was analyzed. Contrary to PNTA1 and 22Rv1 cells, MLT improved the proliferation of PC3 cells in hyperglycemic medium. The combined data indicated that MLT had proliferative and antiapoptotic effects in prostate cells subjected to HG levels and it seems to involve specific MLT pathways rather than AR.http://dx.doi.org/10.1155/2015/538529 |
| spellingShingle | Marina G. Gobbo Nishtman Dizeyi Per-Anders Abrahamsson Per-Anders Bertilsson Viviane Sanches Masitéli Eloisa Zanin Pytlowanciv Sebastião R. Taboga Rejane M. Góes Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic Conditions Journal of Diabetes Research |
| title | Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic Conditions |
| title_full | Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic Conditions |
| title_fullStr | Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic Conditions |
| title_full_unstemmed | Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic Conditions |
| title_short | Influence of Melatonin on the Proliferative and Apoptotic Responses of the Prostate under Normal and Hyperglycemic Conditions |
| title_sort | influence of melatonin on the proliferative and apoptotic responses of the prostate under normal and hyperglycemic conditions |
| url | http://dx.doi.org/10.1155/2015/538529 |
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