Reprogramming CAR-T cells: Targeting SLC transporters for tumor microenvironment adaptation

T cell activation, triggered by antigen recognition via the T cell receptor, initiates crucial physiological changes necessary for proliferation and survival. This process encompasses epigenetic modifications that open chromatin for transcription factor binding, transcriptomic shifts activating key...

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Main Authors: Beatriz Perucha, Maialen Martinez-Tabar, Inés Sanchez-Moreno, Pedro Justicia, Teresa Lozano, Juan José Lasarte
Format: Article
Language:English
Published: Elsevier 2025-10-01
Series:Pharmacological Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S1043661825003536
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author Beatriz Perucha
Maialen Martinez-Tabar
Inés Sanchez-Moreno
Pedro Justicia
Teresa Lozano
Juan José Lasarte
author_facet Beatriz Perucha
Maialen Martinez-Tabar
Inés Sanchez-Moreno
Pedro Justicia
Teresa Lozano
Juan José Lasarte
author_sort Beatriz Perucha
collection DOAJ
description T cell activation, triggered by antigen recognition via the T cell receptor, initiates crucial physiological changes necessary for proliferation and survival. This process encompasses epigenetic modifications that open chromatin for transcription factor binding, transcriptomic shifts activating key genes, and structural adaptations, including increased cell size and cytoskeletal changes. Activated T cells experience heightened metabolic demands, requiring efficient nutrient uptake primarily via Solute Carrier (SLC) transporters. These transporters, regulated by transcription factors such as c-Myc, HIF-1α, NF-κB, and mTORC1, optimize nutrient acquisition to support glycolysis and macromolecule synthesis. In the tumor microenvironment, however, aberrant tumor metabolism depletes nutrients and produces metabolites that impair T cell function and reduce the efficacy of adoptive cell therapies, which are not metabolically adapted to survive in such hostile conditions. Modulating SLC transporters presents a promising strategy to enhance chimeric antigen receptor T cell (CAR-T) effectiveness by improving their persistence and antitumor activity. Personalized approaches targeting SLC transporters, tailored to the metabolic profiles of specific tumor types, are crucial for maximizing the therapeutic potential of adoptive T cell therapies. This review explores the critical role of SLC transporters in CAR-T cell functionality and discusses strategies to optimize their expression and activity within the challenging TME.
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spelling doaj-art-9c1b340138454870a5dd6978ecb121dc2025-08-26T04:14:09ZengElsevierPharmacological Research1096-11862025-10-0122010792810.1016/j.phrs.2025.107928Reprogramming CAR-T cells: Targeting SLC transporters for tumor microenvironment adaptationBeatriz Perucha0Maialen Martinez-Tabar1Inés Sanchez-Moreno2Pedro Justicia3Teresa Lozano4Juan José Lasarte5Program of Immunology and Immunotherapy, Cima Universidad de Navarra, CCUN, Pamplona, SpainProgram of Immunology and Immunotherapy, Cima Universidad de Navarra, CCUN, Pamplona, SpainProgram of Immunology and Immunotherapy, Cima Universidad de Navarra, CCUN, Pamplona, SpainProgram of Immunology and Immunotherapy, Cima Universidad de Navarra, CCUN, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, SpainProgram of Immunology and Immunotherapy, Cima Universidad de Navarra, CCUN, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, Spain; Corresponding authors at: Program of Immunology and Immunotherapy, Cima Universidad de Navarra, Pamplona, Spain.Program of Immunology and Immunotherapy, Cima Universidad de Navarra, CCUN, Pamplona, Spain; Navarra Institute for Health Research (IdiSNA), Pamplona, Spain; Corresponding authors at: Program of Immunology and Immunotherapy, Cima Universidad de Navarra, Pamplona, Spain.T cell activation, triggered by antigen recognition via the T cell receptor, initiates crucial physiological changes necessary for proliferation and survival. This process encompasses epigenetic modifications that open chromatin for transcription factor binding, transcriptomic shifts activating key genes, and structural adaptations, including increased cell size and cytoskeletal changes. Activated T cells experience heightened metabolic demands, requiring efficient nutrient uptake primarily via Solute Carrier (SLC) transporters. These transporters, regulated by transcription factors such as c-Myc, HIF-1α, NF-κB, and mTORC1, optimize nutrient acquisition to support glycolysis and macromolecule synthesis. In the tumor microenvironment, however, aberrant tumor metabolism depletes nutrients and produces metabolites that impair T cell function and reduce the efficacy of adoptive cell therapies, which are not metabolically adapted to survive in such hostile conditions. Modulating SLC transporters presents a promising strategy to enhance chimeric antigen receptor T cell (CAR-T) effectiveness by improving their persistence and antitumor activity. Personalized approaches targeting SLC transporters, tailored to the metabolic profiles of specific tumor types, are crucial for maximizing the therapeutic potential of adoptive T cell therapies. This review explores the critical role of SLC transporters in CAR-T cell functionality and discusses strategies to optimize their expression and activity within the challenging TME.http://www.sciencedirect.com/science/article/pii/S1043661825003536Solute carrier transportersTumor metabolismTumor microenvironmentImmunotherapyCAR-T cellsSolid tumors
spellingShingle Beatriz Perucha
Maialen Martinez-Tabar
Inés Sanchez-Moreno
Pedro Justicia
Teresa Lozano
Juan José Lasarte
Reprogramming CAR-T cells: Targeting SLC transporters for tumor microenvironment adaptation
Pharmacological Research
Solute carrier transporters
Tumor metabolism
Tumor microenvironment
Immunotherapy
CAR-T cells
Solid tumors
title Reprogramming CAR-T cells: Targeting SLC transporters for tumor microenvironment adaptation
title_full Reprogramming CAR-T cells: Targeting SLC transporters for tumor microenvironment adaptation
title_fullStr Reprogramming CAR-T cells: Targeting SLC transporters for tumor microenvironment adaptation
title_full_unstemmed Reprogramming CAR-T cells: Targeting SLC transporters for tumor microenvironment adaptation
title_short Reprogramming CAR-T cells: Targeting SLC transporters for tumor microenvironment adaptation
title_sort reprogramming car t cells targeting slc transporters for tumor microenvironment adaptation
topic Solute carrier transporters
Tumor metabolism
Tumor microenvironment
Immunotherapy
CAR-T cells
Solid tumors
url http://www.sciencedirect.com/science/article/pii/S1043661825003536
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