The GhANT-GoPGF module regulates pigment gland development in cotton leaves
Summary: Gossypium spp. pigment glands are a good model for studying plant secretory cavity structures. GoPGF (GOSSYPIUM PIGMENT GLAND FORMATION) is a well-characterized master transcription factor that controls gland formation in cotton; however, little is known about its transcriptional regulation...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124724014633 |
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| author | Lu Long Zhen-Nan Zhang Fu-Chun Xu Jia-Yi Ma Shen-Zhai Shang Hao-Ge Song Jian-Feng Wu Xiao-Tong Zhao Jose Ramon Botella Shuang-Xia Jin Wei Gao |
| author_facet | Lu Long Zhen-Nan Zhang Fu-Chun Xu Jia-Yi Ma Shen-Zhai Shang Hao-Ge Song Jian-Feng Wu Xiao-Tong Zhao Jose Ramon Botella Shuang-Xia Jin Wei Gao |
| author_sort | Lu Long |
| collection | DOAJ |
| description | Summary: Gossypium spp. pigment glands are a good model for studying plant secretory cavity structures. GoPGF (GOSSYPIUM PIGMENT GLAND FORMATION) is a well-characterized master transcription factor that controls gland formation in cotton; however, little is known about its transcriptional regulation. This study integrates yeast one-hybrid sequencing data and the previously reported single-cell RNA sequencing data to identify upstream GoPGF binding proteins. Several transcription factors preferentially expressed in pigment gland cells (PGCs) are identified, including the cotton AINTEGUMENTA ortholog GhANT. Silencing of GhANT produces a defective leaf-specific PGC phenotype. Knockdown of GhANT reduces mesophyll gland number and gossypol production, while CRISPR-mediated GhANT knockout suppresses mesophyll development. Overexpression of GhANT increases organ size but not cell size. GhANT binds to two CCG boxes in the GoPGF promoter to trigger GoPGF-GhJUB1-regulated gland formation. Our study dissects the subtle regulation of tissue-specific gland morphogenesis in cotton and provides molecular mechanisms to study secretory cavity structures widespread among vascular plants. |
| format | Article |
| id | doaj-art-9c1958f6ce034a778eb62ebcc4b695d2 |
| institution | Kabale University |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-9c1958f6ce034a778eb62ebcc4b695d22024-12-25T04:21:16ZengElsevierCell Reports2211-12472025-01-01441115112The GhANT-GoPGF module regulates pigment gland development in cotton leavesLu Long0Zhen-Nan Zhang1Fu-Chun Xu2Jia-Yi Ma3Shen-Zhai Shang4Hao-Ge Song5Jian-Feng Wu6Xiao-Tong Zhao7Jose Ramon Botella8Shuang-Xia Jin9Wei Gao10National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Life Science, Henan University, Kaifeng, Henan 475004, P.R. ChinaNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Life Science, Henan University, Kaifeng, Henan 475004, P.R. ChinaChangzhi Medical College, Changzhi, Shanxi 046000, P.R. ChinaNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Life Science, Henan University, Kaifeng, Henan 475004, P.R. ChinaNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Life Science, Henan University, Kaifeng, Henan 475004, P.R. ChinaNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Life Science, Henan University, Kaifeng, Henan 475004, P.R. ChinaNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Life Science, Henan University, Kaifeng, Henan 475004, P.R. ChinaNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Life Science, Henan University, Kaifeng, Henan 475004, P.R. ChinaPlant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, The University of Queensland, St. Lucia, Brisbane, QLD 4072, AustraliaHubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, P.R. China; Corresponding authorNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Life Science, Henan University, Kaifeng, Henan 475004, P.R. China; Corresponding authorSummary: Gossypium spp. pigment glands are a good model for studying plant secretory cavity structures. GoPGF (GOSSYPIUM PIGMENT GLAND FORMATION) is a well-characterized master transcription factor that controls gland formation in cotton; however, little is known about its transcriptional regulation. This study integrates yeast one-hybrid sequencing data and the previously reported single-cell RNA sequencing data to identify upstream GoPGF binding proteins. Several transcription factors preferentially expressed in pigment gland cells (PGCs) are identified, including the cotton AINTEGUMENTA ortholog GhANT. Silencing of GhANT produces a defective leaf-specific PGC phenotype. Knockdown of GhANT reduces mesophyll gland number and gossypol production, while CRISPR-mediated GhANT knockout suppresses mesophyll development. Overexpression of GhANT increases organ size but not cell size. GhANT binds to two CCG boxes in the GoPGF promoter to trigger GoPGF-GhJUB1-regulated gland formation. Our study dissects the subtle regulation of tissue-specific gland morphogenesis in cotton and provides molecular mechanisms to study secretory cavity structures widespread among vascular plants.http://www.sciencedirect.com/science/article/pii/S2211124724014633CP: Plants |
| spellingShingle | Lu Long Zhen-Nan Zhang Fu-Chun Xu Jia-Yi Ma Shen-Zhai Shang Hao-Ge Song Jian-Feng Wu Xiao-Tong Zhao Jose Ramon Botella Shuang-Xia Jin Wei Gao The GhANT-GoPGF module regulates pigment gland development in cotton leaves Cell Reports CP: Plants |
| title | The GhANT-GoPGF module regulates pigment gland development in cotton leaves |
| title_full | The GhANT-GoPGF module regulates pigment gland development in cotton leaves |
| title_fullStr | The GhANT-GoPGF module regulates pigment gland development in cotton leaves |
| title_full_unstemmed | The GhANT-GoPGF module regulates pigment gland development in cotton leaves |
| title_short | The GhANT-GoPGF module regulates pigment gland development in cotton leaves |
| title_sort | ghant gopgf module regulates pigment gland development in cotton leaves |
| topic | CP: Plants |
| url | http://www.sciencedirect.com/science/article/pii/S2211124724014633 |
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