Clinical Validation of a Highly Sensitive GC-MS Platform for Routine Urine Drug Screening and Real-Time Reporting of up to 212 Drugs
An important role of the clinical toxicology laboratory is to provide continuous diagnostic testing for patients with altered mental status and for other medical indications. To meet these needs, we have developed a new Gas Chromatography-Mass Spectrometry (GC-MS) platform that facilitates routine s...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2013/329407 |
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| author | Hari Nair Fred Woo Andrew N. Hoofnagle Geoffrey S. Baird |
| author_facet | Hari Nair Fred Woo Andrew N. Hoofnagle Geoffrey S. Baird |
| author_sort | Hari Nair |
| collection | DOAJ |
| description | An important role of the clinical toxicology laboratory is to provide continuous diagnostic testing for patients with altered mental status and for other medical indications. To meet these needs, we have developed a new Gas Chromatography-Mass Spectrometry (GC-MS) platform that facilitates routine screening and automated reporting of 212 drugs by laboratory technologists around the clock without the need to sign out by an on-site mass spectrometry-trained toxicologist. The platform uses a programmable temperature vaporizer (PTV) injector for large sample volume injection and the free software Automated Mass Spectral Deconvolution and Identification System (AMDIS) for data reduction and spectral matching that facilitates rapid library searching and analyte identification. Method comparison with 118 patient samples demonstrated that this platform and data searching algorithm independently provided improvements in sensitivity compared to an established GC-MS platform. Further examination of the role of the data processing software and the in-house databases used in the established versus the new platform demonstrated that the improved analytical sensitivity of the new platform was attributed to both the technical superiority of the new GC-MS instrumentation and the use of AMDIS in conjunction with the newly generated in-house library for data processing. |
| format | Article |
| id | doaj-art-9c15f0aecaf0459e9afd1f9f22794f23 |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-9c15f0aecaf0459e9afd1f9f22794f232025-08-20T03:38:22ZengWileyJournal of Toxicology1687-81911687-82052013-01-01201310.1155/2013/329407329407Clinical Validation of a Highly Sensitive GC-MS Platform for Routine Urine Drug Screening and Real-Time Reporting of up to 212 DrugsHari Nair0Fred Woo1Andrew N. Hoofnagle2Geoffrey S. Baird3Department of Laboratory Medicine, University of Washington, P.O. Box 357110, 1959 NE Pacific Street, Seattle, WA 98185, USADepartment of Laboratory Medicine, University of Washington, P.O. Box 357110, 1959 NE Pacific Street, Seattle, WA 98185, USADepartment of Laboratory Medicine, University of Washington, P.O. Box 357110, 1959 NE Pacific Street, Seattle, WA 98185, USADepartment of Laboratory Medicine, University of Washington, P.O. Box 357110, 1959 NE Pacific Street, Seattle, WA 98185, USAAn important role of the clinical toxicology laboratory is to provide continuous diagnostic testing for patients with altered mental status and for other medical indications. To meet these needs, we have developed a new Gas Chromatography-Mass Spectrometry (GC-MS) platform that facilitates routine screening and automated reporting of 212 drugs by laboratory technologists around the clock without the need to sign out by an on-site mass spectrometry-trained toxicologist. The platform uses a programmable temperature vaporizer (PTV) injector for large sample volume injection and the free software Automated Mass Spectral Deconvolution and Identification System (AMDIS) for data reduction and spectral matching that facilitates rapid library searching and analyte identification. Method comparison with 118 patient samples demonstrated that this platform and data searching algorithm independently provided improvements in sensitivity compared to an established GC-MS platform. Further examination of the role of the data processing software and the in-house databases used in the established versus the new platform demonstrated that the improved analytical sensitivity of the new platform was attributed to both the technical superiority of the new GC-MS instrumentation and the use of AMDIS in conjunction with the newly generated in-house library for data processing.http://dx.doi.org/10.1155/2013/329407 |
| spellingShingle | Hari Nair Fred Woo Andrew N. Hoofnagle Geoffrey S. Baird Clinical Validation of a Highly Sensitive GC-MS Platform for Routine Urine Drug Screening and Real-Time Reporting of up to 212 Drugs Journal of Toxicology |
| title | Clinical Validation of a Highly Sensitive GC-MS Platform for Routine Urine Drug Screening and Real-Time Reporting of up to 212 Drugs |
| title_full | Clinical Validation of a Highly Sensitive GC-MS Platform for Routine Urine Drug Screening and Real-Time Reporting of up to 212 Drugs |
| title_fullStr | Clinical Validation of a Highly Sensitive GC-MS Platform for Routine Urine Drug Screening and Real-Time Reporting of up to 212 Drugs |
| title_full_unstemmed | Clinical Validation of a Highly Sensitive GC-MS Platform for Routine Urine Drug Screening and Real-Time Reporting of up to 212 Drugs |
| title_short | Clinical Validation of a Highly Sensitive GC-MS Platform for Routine Urine Drug Screening and Real-Time Reporting of up to 212 Drugs |
| title_sort | clinical validation of a highly sensitive gc ms platform for routine urine drug screening and real time reporting of up to 212 drugs |
| url | http://dx.doi.org/10.1155/2013/329407 |
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