Correlation of spectroscopic and structural properties of indocyanine green j-aggregates

Indocyanine green (ICG), when in free form in a liquid, can form stable nanoparticle structures or colloidal solution, while changing its spectroscopic properties. In the work, the aggregation degree and the average size of nanoparticles depending on the concentration of a colloidal solution of indo...

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Main Authors: D. S. Farrakhova, I. D. Romanishkin, D. V. Yakovlev, Yu. S. Maklygina, V. A. Oleinikov, P. V. Fedotov, M. V. Kravchik, L. Bezdetnaya, V. B. Loschenov
Format: Article
Language:Russian
Published: Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association" 2022-11-01
Series:Biomedical Photonics
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Online Access:https://www.pdt-journal.com/jour/article/view/548
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author D. S. Farrakhova
I. D. Romanishkin
D. V. Yakovlev
Yu. S. Maklygina
V. A. Oleinikov
P. V. Fedotov
M. V. Kravchik
L. Bezdetnaya
V. B. Loschenov
author_facet D. S. Farrakhova
I. D. Romanishkin
D. V. Yakovlev
Yu. S. Maklygina
V. A. Oleinikov
P. V. Fedotov
M. V. Kravchik
L. Bezdetnaya
V. B. Loschenov
author_sort D. S. Farrakhova
collection DOAJ
description Indocyanine green (ICG), when in free form in a liquid, can form stable nanoparticle structures or colloidal solution, while changing its spectroscopic properties. In the work, the aggregation degree and the average size of nanoparticles depending on the concentration of a colloidal solution of indocyanine green (ICG NPs) in the form of J-aggregates were investigated by various methods based on light scattering. The size of nanoparticles is an important parameter from the point of view of clinical application, because the technique of intravenous administration of drugs, in order to avoid microvascular thrombosis and embolism, provides dosage forms with inclusions of individual molecules or their clusters, not exceeding 500 nm diameter. In turn, small nanoparticles less than 30 nm lead to prolonged circulation of the drug in the body with an increased possibility of permeation into cells of healthy tissue. In the course of studies, it was found that an increase in the concentration of ICG NPs in the solution leads to an increase in the average size of spontaneously formed J-aggregates, which, in turn, leads to a decrease in the absorption coefficient in the aggregates. Presumably, this phenomenon, i.e. the established nonlinear dependence of the J-aggregate absorption on its size, can be explained by the formation of absorption centers on the J-aggregate surface in the form of mobile surface molecules. The threshold range of ICG molecule concentration was determined, at which there is a transition from aggregation with an increase in size with a slow addition of ICG J-aggregate molecules in height to a rapid addition in width.
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issn 2413-9432
language Russian
publishDate 2022-11-01
publisher Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"
record_format Article
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spelling doaj-art-9c07fcaaff8340ce8166f67920e4e4c52025-08-20T03:21:05ZrusNon-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"Biomedical Photonics2413-94322022-11-0111341610.24931/2413-9432-2022-11-3-4-16256Correlation of spectroscopic and structural properties of indocyanine green j-aggregatesD. S. Farrakhova0I. D. Romanishkin1D. V. Yakovlev2Yu. S. Maklygina3V. A. Oleinikov4P. V. Fedotov5M. V. Kravchik6L. Bezdetnaya7V. B. Loschenov8Prokhorov General Physics Institute of the Russian Academy of ScienceProkhorov General Physics Institute of the Russian Academy of ScienceProkhorov General Physics Institute of the Russian Academy of Science,Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of ScienceProkhorov General Physics Institute of the Russian Academy of ScienceShemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of ScienceProkhorov General Physics Institute of the Russian Academy of Science, Moscow Institute of Physics and TechnologyResearch Institute of Eye DiseasesInstitut de Cancèrologie de Lorraine, Centre de Recherche en Automatique de Nancy, CNRSProkhorov General Physics Institute of the Russian Academy of ScienceIndocyanine green (ICG), when in free form in a liquid, can form stable nanoparticle structures or colloidal solution, while changing its spectroscopic properties. In the work, the aggregation degree and the average size of nanoparticles depending on the concentration of a colloidal solution of indocyanine green (ICG NPs) in the form of J-aggregates were investigated by various methods based on light scattering. The size of nanoparticles is an important parameter from the point of view of clinical application, because the technique of intravenous administration of drugs, in order to avoid microvascular thrombosis and embolism, provides dosage forms with inclusions of individual molecules or their clusters, not exceeding 500 nm diameter. In turn, small nanoparticles less than 30 nm lead to prolonged circulation of the drug in the body with an increased possibility of permeation into cells of healthy tissue. In the course of studies, it was found that an increase in the concentration of ICG NPs in the solution leads to an increase in the average size of spontaneously formed J-aggregates, which, in turn, leads to a decrease in the absorption coefficient in the aggregates. Presumably, this phenomenon, i.e. the established nonlinear dependence of the J-aggregate absorption on its size, can be explained by the formation of absorption centers on the J-aggregate surface in the form of mobile surface molecules. The threshold range of ICG molecule concentration was determined, at which there is a transition from aggregation with an increase in size with a slow addition of ICG J-aggregate molecules in height to a rapid addition in width.https://www.pdt-journal.com/jour/article/view/548mie scatteringdynamic light scatteringindocyanine greencolloidal solutionj-aggregatesscattering indicatrixaggregation degree
spellingShingle D. S. Farrakhova
I. D. Romanishkin
D. V. Yakovlev
Yu. S. Maklygina
V. A. Oleinikov
P. V. Fedotov
M. V. Kravchik
L. Bezdetnaya
V. B. Loschenov
Correlation of spectroscopic and structural properties of indocyanine green j-aggregates
Biomedical Photonics
mie scattering
dynamic light scattering
indocyanine green
colloidal solution
j-aggregates
scattering indicatrix
aggregation degree
title Correlation of spectroscopic and structural properties of indocyanine green j-aggregates
title_full Correlation of spectroscopic and structural properties of indocyanine green j-aggregates
title_fullStr Correlation of spectroscopic and structural properties of indocyanine green j-aggregates
title_full_unstemmed Correlation of spectroscopic and structural properties of indocyanine green j-aggregates
title_short Correlation of spectroscopic and structural properties of indocyanine green j-aggregates
title_sort correlation of spectroscopic and structural properties of indocyanine green j aggregates
topic mie scattering
dynamic light scattering
indocyanine green
colloidal solution
j-aggregates
scattering indicatrix
aggregation degree
url https://www.pdt-journal.com/jour/article/view/548
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AT yusmaklygina correlationofspectroscopicandstructuralpropertiesofindocyaninegreenjaggregates
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AT pvfedotov correlationofspectroscopicandstructuralpropertiesofindocyaninegreenjaggregates
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