Clinical characterization of CCT2 and its role in autophagy regulation during age-related macular degeneration
Abstract Age-related macular degeneration (AMD) is the leading cause of vision loss in the elderly, and the role of chaperonin containing TCP1 subunit 2 (CCT2) remains unclear. This study aims to elucidate the mechanistic link between CCT2 and AMD, contributing to improved understanding and potentia...
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| Language: | English |
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Nature Portfolio
2025-05-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-01907-1 |
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| author | Lin Wang Ling-xiao Wang Mu-ye Li Rong Zhang Guo-hong Zhou |
| author_facet | Lin Wang Ling-xiao Wang Mu-ye Li Rong Zhang Guo-hong Zhou |
| author_sort | Lin Wang |
| collection | DOAJ |
| description | Abstract Age-related macular degeneration (AMD) is the leading cause of vision loss in the elderly, and the role of chaperonin containing TCP1 subunit 2 (CCT2) remains unclear. This study aims to elucidate the mechanistic link between CCT2 and AMD, contributing to improved understanding and potential therapeutic strategies. Retinal and RPE-Choroid transcriptome array data from 130 AMD patients and 121 normal donors (GSE29801 dataset) were reanalyzed to assess CCT2 expression across different AMD subtypes, age groups, and genders. Single-sample gene set enrichment analysis was performed to explore correlations with autophagy-related genes and other established AMD causes. Additionally, CCT2 expression was validated in sodium iodate (NaIO₃)-induced 661 W cells (photoreceptor-like cells) using quantitative real-time PCR (qRT-PCR). CCT2 was significantly enriched in advanced AMD retinas compared to intermediate stages in retina (both macular and extramacular) and early stages in extramacular retina (p < 0.05). NaIO3-treated 661 W cells exhibited a similar expression trend, confirming transcriptomic findings. CCT2 is significantly upregulated in advanced AMD and may contribute to drusen degradation. It shows potential as both a biomarker and an independent diagnostic indicator, particularly for advanced-stage AMD. |
| format | Article |
| id | doaj-art-9bfc6bb4437c49f49810cfba76ef313c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-9bfc6bb4437c49f49810cfba76ef313c2025-08-20T03:48:02ZengNature PortfolioScientific Reports2045-23222025-05-0115111410.1038/s41598-025-01907-1Clinical characterization of CCT2 and its role in autophagy regulation during age-related macular degenerationLin Wang0Ling-xiao Wang1Mu-ye Li2Rong Zhang3Guo-hong Zhou4Department of Ophthalmology, Shanxi Eye Hospital Affiliated to Shanxi Medical UniversityDepartment of Colorectal and Anal Surgery, Shanxi Provincial People’s Hospital (Fifth Hospital of Shanxi Medical University)Department of Ophthalmology, Shanxi Eye Hospital Affiliated to Shanxi Medical UniversityDepartment of Ophthalmology, Shanxi Eye Hospital Affiliated to Shanxi Medical UniversityDepartment of Ophthalmology, Shanxi Eye Hospital Affiliated to Shanxi Medical UniversityAbstract Age-related macular degeneration (AMD) is the leading cause of vision loss in the elderly, and the role of chaperonin containing TCP1 subunit 2 (CCT2) remains unclear. This study aims to elucidate the mechanistic link between CCT2 and AMD, contributing to improved understanding and potential therapeutic strategies. Retinal and RPE-Choroid transcriptome array data from 130 AMD patients and 121 normal donors (GSE29801 dataset) were reanalyzed to assess CCT2 expression across different AMD subtypes, age groups, and genders. Single-sample gene set enrichment analysis was performed to explore correlations with autophagy-related genes and other established AMD causes. Additionally, CCT2 expression was validated in sodium iodate (NaIO₃)-induced 661 W cells (photoreceptor-like cells) using quantitative real-time PCR (qRT-PCR). CCT2 was significantly enriched in advanced AMD retinas compared to intermediate stages in retina (both macular and extramacular) and early stages in extramacular retina (p < 0.05). NaIO3-treated 661 W cells exhibited a similar expression trend, confirming transcriptomic findings. CCT2 is significantly upregulated in advanced AMD and may contribute to drusen degradation. It shows potential as both a biomarker and an independent diagnostic indicator, particularly for advanced-stage AMD.https://doi.org/10.1038/s41598-025-01907-1Age related macular degenerationNeurodegenerationCCT2AutophagyDrusen |
| spellingShingle | Lin Wang Ling-xiao Wang Mu-ye Li Rong Zhang Guo-hong Zhou Clinical characterization of CCT2 and its role in autophagy regulation during age-related macular degeneration Scientific Reports Age related macular degeneration Neurodegeneration CCT2 Autophagy Drusen |
| title | Clinical characterization of CCT2 and its role in autophagy regulation during age-related macular degeneration |
| title_full | Clinical characterization of CCT2 and its role in autophagy regulation during age-related macular degeneration |
| title_fullStr | Clinical characterization of CCT2 and its role in autophagy regulation during age-related macular degeneration |
| title_full_unstemmed | Clinical characterization of CCT2 and its role in autophagy regulation during age-related macular degeneration |
| title_short | Clinical characterization of CCT2 and its role in autophagy regulation during age-related macular degeneration |
| title_sort | clinical characterization of cct2 and its role in autophagy regulation during age related macular degeneration |
| topic | Age related macular degeneration Neurodegeneration CCT2 Autophagy Drusen |
| url | https://doi.org/10.1038/s41598-025-01907-1 |
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