Tumour-Associated Transcripts and EGFR Deletion Variants in Colorectal Cancer in Primary Tumour, Metastases and Circulating Tumour Cells
The clinical relevance of circulating tumour cells (CTC) in peripheral blood of patients with colorectal cancer (CRC) has been described as an independent prognostic factor useful to monitor drug effects and clinical status. The aim of the present study was to compare the epidermal growth factor rec...
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| Format: | Article |
| Language: | English |
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Wiley
2008-01-01
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| Series: | Cellular Oncology |
| Online Access: | http://dx.doi.org/10.3233/CLO-2008-0432 |
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| author | Silke Lankiewicz Eva Rother Silke Zimmermann Christiane Hollmann Firouzeh Korangy Tim F. Greten |
| author_facet | Silke Lankiewicz Eva Rother Silke Zimmermann Christiane Hollmann Firouzeh Korangy Tim F. Greten |
| author_sort | Silke Lankiewicz |
| collection | DOAJ |
| description | The clinical relevance of circulating tumour cells (CTC) in peripheral blood of patients with colorectal cancer (CRC) has been described as an independent prognostic factor useful to monitor drug effects and clinical status. The aim of the present study was to compare the epidermal growth factor receptor (EGFR) status of primary tumour, related metastases and CTC of patients with CRC. Therefore, in addition to EGFR, the tumour-associated transcripts gastrointestinal tumour-associated antigen 733-2 (GA733-2) and carcinoembryonic antigen (CEA) were analyzed in a multiplex RT-PCR to characterize CTC. 55% patients were positive for CTC. EGFR expression was detected in 18% of these patients. EGFR was expressed more frequently in metastatic and primary tumour tissues as revealed by immunohistochemistry. Besides, detailed expression profiling of EGFR variants in various colorectal and glioma cell lines has been performed to generate positive controls, resulting in the discovery of two new transcript deletion variations (cEX12_15del, cEX12_14del) located on the extracellular domain of the EGFR. |
| format | Article |
| id | doaj-art-9bf4598067b64c12b48a78183ef852ab |
| institution | Kabale University |
| issn | 1570-5870 1875-8606 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cellular Oncology |
| spelling | doaj-art-9bf4598067b64c12b48a78183ef852ab2025-02-03T05:55:16ZengWileyCellular Oncology1570-58701875-86062008-01-0130646347110.3233/CLO-2008-0432Tumour-Associated Transcripts and EGFR Deletion Variants in Colorectal Cancer in Primary Tumour, Metastases and Circulating Tumour CellsSilke Lankiewicz0Eva Rother1Silke Zimmermann2Christiane Hollmann3Firouzeh Korangy4Tim F. Greten5AdnaGen AG, D-30853 Langenhagen, GermanyDepartment of Gastroenterology, Hepatology and Endocrinology, Centre for Internal Medicine, Hannover Medical School, D-30625 Hannover, GermanyAdnaGen AG, D-30853 Langenhagen, GermanyAdnaGen AG, D-30853 Langenhagen, GermanyDepartment of Gastroenterology, Hepatology and Endocrinology, Centre for Internal Medicine, Hannover Medical School, D-30625 Hannover, GermanyDepartment of Gastroenterology, Hepatology and Endocrinology, Centre for Internal Medicine, Hannover Medical School, D-30625 Hannover, GermanyThe clinical relevance of circulating tumour cells (CTC) in peripheral blood of patients with colorectal cancer (CRC) has been described as an independent prognostic factor useful to monitor drug effects and clinical status. The aim of the present study was to compare the epidermal growth factor receptor (EGFR) status of primary tumour, related metastases and CTC of patients with CRC. Therefore, in addition to EGFR, the tumour-associated transcripts gastrointestinal tumour-associated antigen 733-2 (GA733-2) and carcinoembryonic antigen (CEA) were analyzed in a multiplex RT-PCR to characterize CTC. 55% patients were positive for CTC. EGFR expression was detected in 18% of these patients. EGFR was expressed more frequently in metastatic and primary tumour tissues as revealed by immunohistochemistry. Besides, detailed expression profiling of EGFR variants in various colorectal and glioma cell lines has been performed to generate positive controls, resulting in the discovery of two new transcript deletion variations (cEX12_15del, cEX12_14del) located on the extracellular domain of the EGFR.http://dx.doi.org/10.3233/CLO-2008-0432 |
| spellingShingle | Silke Lankiewicz Eva Rother Silke Zimmermann Christiane Hollmann Firouzeh Korangy Tim F. Greten Tumour-Associated Transcripts and EGFR Deletion Variants in Colorectal Cancer in Primary Tumour, Metastases and Circulating Tumour Cells Cellular Oncology |
| title | Tumour-Associated Transcripts and EGFR Deletion Variants in Colorectal Cancer in Primary Tumour, Metastases and Circulating Tumour Cells |
| title_full | Tumour-Associated Transcripts and EGFR Deletion Variants in Colorectal Cancer in Primary Tumour, Metastases and Circulating Tumour Cells |
| title_fullStr | Tumour-Associated Transcripts and EGFR Deletion Variants in Colorectal Cancer in Primary Tumour, Metastases and Circulating Tumour Cells |
| title_full_unstemmed | Tumour-Associated Transcripts and EGFR Deletion Variants in Colorectal Cancer in Primary Tumour, Metastases and Circulating Tumour Cells |
| title_short | Tumour-Associated Transcripts and EGFR Deletion Variants in Colorectal Cancer in Primary Tumour, Metastases and Circulating Tumour Cells |
| title_sort | tumour associated transcripts and egfr deletion variants in colorectal cancer in primary tumour metastases and circulating tumour cells |
| url | http://dx.doi.org/10.3233/CLO-2008-0432 |
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