High-Stringency Subtraction for the Identification of Differentially Regulated cDNA Clones
The technique of high-stringency subtraction described here facilitates subtractive hybridizations between directional cDNA libraries constructed in λ ZAP® II cloning vectors and represents an improvement on earlier methods for the subtraction of entire cDNA libraries. High-stringency subtraction is...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97233st04 |
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