Hypothyroidism Affects Vascularization and Promotes Immune Cells Infiltration into Pancreatic Islets of Female Rabbits
Thyroidectomy induces pancreatic edema and immune cells infiltration similarly to that observed in pancreatitis. In spite of the controverted effects of hypothyroidism on serum glucose and insulin concentrations, the number and proliferation of Langerhans islet cells as well as the presence of extra...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2015/917806 |
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| author | Julia Rodríguez-Castelán Margarita Martínez-Gómez Francisco Castelán Estela Cuevas |
| author_facet | Julia Rodríguez-Castelán Margarita Martínez-Gómez Francisco Castelán Estela Cuevas |
| author_sort | Julia Rodríguez-Castelán |
| collection | DOAJ |
| description | Thyroidectomy induces pancreatic edema and immune cells infiltration similarly to that observed in pancreatitis. In spite of the controverted effects of hypothyroidism on serum glucose and insulin concentrations, the number and proliferation of Langerhans islet cells as well as the presence of extracellular matrix are affected depending on the islet size. In this study, we evaluated the effect of methimazole-induced hypothyroidism on the vascularization and immune cells infiltration into islets. A general observation of pancreas was also done. Twelve Chinchilla-breed female adult rabbits were divided into control n=6 and hypothyroid groups (n=6, methimazole, 0.02% in drinking water for 30 days). After the treatment, rabbits were sacrificed and their pancreas was excised, histologically processed, and stained with Periodic Acid-Schiff (PAS) or Masson’s Trichrome techniques. Islets were arbitrarily classified into large, medium, and small ones. The external and internal portions of each islet were also identified. Student-t-test and Mann-Whitney-U test or two-way ANOVAs were used to compare variables between groups. In comparison with control rabbits, hypothyroidism induced a strong infiltration of immune cells and a major presence of collagen and proteoglycans in the interlobular septa. Large islets showed a high vascularization and immune cells infiltration. The present results show that hypothyroidism induces pancreatitis and insulitis. |
| format | Article |
| id | doaj-art-9bdb02cf837c41a08fef191856889196 |
| institution | OA Journals |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-9bdb02cf837c41a08fef1918568891962025-08-20T02:18:25ZengWileyInternational Journal of Endocrinology1687-83371687-83452015-01-01201510.1155/2015/917806917806Hypothyroidism Affects Vascularization and Promotes Immune Cells Infiltration into Pancreatic Islets of Female RabbitsJulia Rodríguez-Castelán0Margarita Martínez-Gómez1Francisco Castelán2Estela Cuevas3Doctorado en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, 90070 Tlaxcala, TLAX, MexicoCentro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, 90070 Tlaxcala, TLAX, MexicoCentro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, 90070 Tlaxcala, TLAX, MexicoCentro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, 90070 Tlaxcala, TLAX, MexicoThyroidectomy induces pancreatic edema and immune cells infiltration similarly to that observed in pancreatitis. In spite of the controverted effects of hypothyroidism on serum glucose and insulin concentrations, the number and proliferation of Langerhans islet cells as well as the presence of extracellular matrix are affected depending on the islet size. In this study, we evaluated the effect of methimazole-induced hypothyroidism on the vascularization and immune cells infiltration into islets. A general observation of pancreas was also done. Twelve Chinchilla-breed female adult rabbits were divided into control n=6 and hypothyroid groups (n=6, methimazole, 0.02% in drinking water for 30 days). After the treatment, rabbits were sacrificed and their pancreas was excised, histologically processed, and stained with Periodic Acid-Schiff (PAS) or Masson’s Trichrome techniques. Islets were arbitrarily classified into large, medium, and small ones. The external and internal portions of each islet were also identified. Student-t-test and Mann-Whitney-U test or two-way ANOVAs were used to compare variables between groups. In comparison with control rabbits, hypothyroidism induced a strong infiltration of immune cells and a major presence of collagen and proteoglycans in the interlobular septa. Large islets showed a high vascularization and immune cells infiltration. The present results show that hypothyroidism induces pancreatitis and insulitis.http://dx.doi.org/10.1155/2015/917806 |
| spellingShingle | Julia Rodríguez-Castelán Margarita Martínez-Gómez Francisco Castelán Estela Cuevas Hypothyroidism Affects Vascularization and Promotes Immune Cells Infiltration into Pancreatic Islets of Female Rabbits International Journal of Endocrinology |
| title | Hypothyroidism Affects Vascularization and Promotes Immune Cells Infiltration into Pancreatic Islets of Female Rabbits |
| title_full | Hypothyroidism Affects Vascularization and Promotes Immune Cells Infiltration into Pancreatic Islets of Female Rabbits |
| title_fullStr | Hypothyroidism Affects Vascularization and Promotes Immune Cells Infiltration into Pancreatic Islets of Female Rabbits |
| title_full_unstemmed | Hypothyroidism Affects Vascularization and Promotes Immune Cells Infiltration into Pancreatic Islets of Female Rabbits |
| title_short | Hypothyroidism Affects Vascularization and Promotes Immune Cells Infiltration into Pancreatic Islets of Female Rabbits |
| title_sort | hypothyroidism affects vascularization and promotes immune cells infiltration into pancreatic islets of female rabbits |
| url | http://dx.doi.org/10.1155/2015/917806 |
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