Assessment of the urinary uric acid-to-creatinine ratio as a diagnostic marker for perinatal asphyxia in a resource-poor setting: a case control study

Abstract Background The lack of simple but effective and affordable diagnostic tools presents a challenge for the management of perinatal asphyxia, especially in low- to middle-income countries. Current diagnostic tools, such as arterial blood gas estimation, are expensive and not readily available...

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Main Authors: Chukwunonso Chigozie Iheji, Isaac Nwabueze Asinobi, Ikenna Kingsley Ndu, Anthony Nnaemeka Ikefuna
Format: Article
Language:English
Published: BMC 2025-08-01
Series:BMC Pediatrics
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Online Access:https://doi.org/10.1186/s12887-025-05981-2
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author Chukwunonso Chigozie Iheji
Isaac Nwabueze Asinobi
Ikenna Kingsley Ndu
Anthony Nnaemeka Ikefuna
author_facet Chukwunonso Chigozie Iheji
Isaac Nwabueze Asinobi
Ikenna Kingsley Ndu
Anthony Nnaemeka Ikefuna
author_sort Chukwunonso Chigozie Iheji
collection DOAJ
description Abstract Background The lack of simple but effective and affordable diagnostic tools presents a challenge for the management of perinatal asphyxia, especially in low- to middle-income countries. Current diagnostic tools, such as arterial blood gas estimation, are expensive and not readily available at primary and secondary levels of care, where most cases of perinatal asphyxia are identified. This causes a delay in diagnosis. Perinatal asphyxia may have improved outcomes if there are cheaper, reliable, and more convenient diagnostic biomarkers that can aid early diagnosis leading to early initiation of treatment. This study assessed the utility of the urinary uric acid-to-urinary creatinine (UUA/UC) ratio in the diagnosis of perinatal asphyxia. Methods This study was conducted among 90 term newborns aged less than 24-hours delivered at delivered at the Enugu State University Teaching Hospital (ESUTH). In the study population, there were an equal number of asphyxiated and apparently healthy babies. Relevant maternal and neonatal histories were obtained, and physical examination was carried out for all enrolled newborns. Umbilical arterial blood was collected for blood gas analysis, and spot urine samples were collected and sent for uric acid and creatinine estimation. Relevant statistical tests were applied in the data analysis. Results The mean UUA/UC ratio was significantly greater in the asphyxiated group (2.41 ± 0.73) than among the control group (0.87 ± 0.29) (t = 13.129, p < 0.001). The accuracy of the UUA/UC ratio in diagnosing perinatal asphyxia had an area under the curve (AUC) of 0.978, implying that the test is accurate. The cut-off point that gives the best diagnosis of perinatal asphyxia was 1.54, with a sensitivity of 95.6%, specificity of 100%, positive predictive value of 100%, and negative predictive value of 96%. Additionally, the UUA/UC ratio has a very strong positive correlation with the severity of hypoxic–ischaemic encephalopathy (HIE) (r = 0.843, p < 0.001). Conclusion The UUA/UC ratio is a good diagnostic marker of asphyxia and varies with the severity of encephalopathy. Accordingly, the urinary uric acid-to-creatinine ratio is recommended as a surrogate biomarker for the diagnosis of perinatal asphyxia.
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spelling doaj-art-9bd17da564434e97bc09e8fa05260c1d2025-08-20T03:46:20ZengBMCBMC Pediatrics1471-24312025-08-012511810.1186/s12887-025-05981-2Assessment of the urinary uric acid-to-creatinine ratio as a diagnostic marker for perinatal asphyxia in a resource-poor setting: a case control studyChukwunonso Chigozie Iheji0Isaac Nwabueze Asinobi1Ikenna Kingsley Ndu2Anthony Nnaemeka Ikefuna3Department of Paediatrics, Enugu State University Teaching HospitalDepartment of Paediatrics, Enugu State University Teaching HospitalDepartment of Paediatrics, Enugu State University Teaching HospitalDepartment of Paediatrics, University of Nigeria Teaching HospitalAbstract Background The lack of simple but effective and affordable diagnostic tools presents a challenge for the management of perinatal asphyxia, especially in low- to middle-income countries. Current diagnostic tools, such as arterial blood gas estimation, are expensive and not readily available at primary and secondary levels of care, where most cases of perinatal asphyxia are identified. This causes a delay in diagnosis. Perinatal asphyxia may have improved outcomes if there are cheaper, reliable, and more convenient diagnostic biomarkers that can aid early diagnosis leading to early initiation of treatment. This study assessed the utility of the urinary uric acid-to-urinary creatinine (UUA/UC) ratio in the diagnosis of perinatal asphyxia. Methods This study was conducted among 90 term newborns aged less than 24-hours delivered at delivered at the Enugu State University Teaching Hospital (ESUTH). In the study population, there were an equal number of asphyxiated and apparently healthy babies. Relevant maternal and neonatal histories were obtained, and physical examination was carried out for all enrolled newborns. Umbilical arterial blood was collected for blood gas analysis, and spot urine samples were collected and sent for uric acid and creatinine estimation. Relevant statistical tests were applied in the data analysis. Results The mean UUA/UC ratio was significantly greater in the asphyxiated group (2.41 ± 0.73) than among the control group (0.87 ± 0.29) (t = 13.129, p < 0.001). The accuracy of the UUA/UC ratio in diagnosing perinatal asphyxia had an area under the curve (AUC) of 0.978, implying that the test is accurate. The cut-off point that gives the best diagnosis of perinatal asphyxia was 1.54, with a sensitivity of 95.6%, specificity of 100%, positive predictive value of 100%, and negative predictive value of 96%. Additionally, the UUA/UC ratio has a very strong positive correlation with the severity of hypoxic–ischaemic encephalopathy (HIE) (r = 0.843, p < 0.001). Conclusion The UUA/UC ratio is a good diagnostic marker of asphyxia and varies with the severity of encephalopathy. Accordingly, the urinary uric acid-to-creatinine ratio is recommended as a surrogate biomarker for the diagnosis of perinatal asphyxia.https://doi.org/10.1186/s12887-025-05981-2Perinatal asphyxiaDiagnosisUrinary uric acidCreatinine
spellingShingle Chukwunonso Chigozie Iheji
Isaac Nwabueze Asinobi
Ikenna Kingsley Ndu
Anthony Nnaemeka Ikefuna
Assessment of the urinary uric acid-to-creatinine ratio as a diagnostic marker for perinatal asphyxia in a resource-poor setting: a case control study
BMC Pediatrics
Perinatal asphyxia
Diagnosis
Urinary uric acid
Creatinine
title Assessment of the urinary uric acid-to-creatinine ratio as a diagnostic marker for perinatal asphyxia in a resource-poor setting: a case control study
title_full Assessment of the urinary uric acid-to-creatinine ratio as a diagnostic marker for perinatal asphyxia in a resource-poor setting: a case control study
title_fullStr Assessment of the urinary uric acid-to-creatinine ratio as a diagnostic marker for perinatal asphyxia in a resource-poor setting: a case control study
title_full_unstemmed Assessment of the urinary uric acid-to-creatinine ratio as a diagnostic marker for perinatal asphyxia in a resource-poor setting: a case control study
title_short Assessment of the urinary uric acid-to-creatinine ratio as a diagnostic marker for perinatal asphyxia in a resource-poor setting: a case control study
title_sort assessment of the urinary uric acid to creatinine ratio as a diagnostic marker for perinatal asphyxia in a resource poor setting a case control study
topic Perinatal asphyxia
Diagnosis
Urinary uric acid
Creatinine
url https://doi.org/10.1186/s12887-025-05981-2
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