Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization
Mammalian sperm delve into the female reproductive tract to fertilize the female gamete. The available information about how sperm regulate their motility during the final journey to the fertilization site is extremely limited. In this work, we investigated the structural and functional changes in t...
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eLife Sciences Publications Ltd
2024-11-01
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| Online Access: | https://elifesciences.org/articles/93792 |
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| author | Martina Jabloñski Guillermina M Luque Matias Gomez Elias Claudia Sanchez Cardenas Xinran Xu Jose L de La Vega Beltran Gabriel Corkidi Alejandro Linares Victor Abonza Aquetzalli Arenas-Hernandez María DP Ramos-Godinez Alejandro López-Saavedra Dario Krapf Diego Krapf Alberto Darszon Adán Guerrero Mariano G Buffone |
| author_facet | Martina Jabloñski Guillermina M Luque Matias Gomez Elias Claudia Sanchez Cardenas Xinran Xu Jose L de La Vega Beltran Gabriel Corkidi Alejandro Linares Victor Abonza Aquetzalli Arenas-Hernandez María DP Ramos-Godinez Alejandro López-Saavedra Dario Krapf Diego Krapf Alberto Darszon Adán Guerrero Mariano G Buffone |
| author_sort | Martina Jabloñski |
| collection | DOAJ |
| description | Mammalian sperm delve into the female reproductive tract to fertilize the female gamete. The available information about how sperm regulate their motility during the final journey to the fertilization site is extremely limited. In this work, we investigated the structural and functional changes in the sperm flagellum after acrosomal exocytosis (AE) and during the interaction with the eggs. The evidence demonstrates that the double helix actin network surrounding the mitochondrial sheath of the midpiece undergoes structural changes prior to the motility cessation. This structural modification is accompanied by a decrease in diameter of the midpiece and is driven by intracellular calcium changes that occur concomitant with a reorganization of the actin helicoidal cortex. Midpiece contraction occurs in a subset of cells that undergo AE, and live-cell imaging during in vitro fertilization showed that the midpiece contraction is required for motility cessation after fusion is initiated. These findings provide the first evidence of the F-actin network’s role in regulating sperm motility, adapting its function to meet specific cellular requirements during fertilization, and highlighting the broader significance of understanding sperm motility. |
| format | Article |
| id | doaj-art-9bcf2ee0892a47569b3a13d8a41cab84 |
| institution | OA Journals |
| issn | 2050-084X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj-art-9bcf2ee0892a47569b3a13d8a41cab842025-08-20T02:14:34ZengeLife Sciences Publications LtdeLife2050-084X2024-11-011310.7554/eLife.93792Reorganization of the flagellum scaffolding induces a sperm standstill during fertilizationMartina Jabloñski0https://orcid.org/0000-0001-6465-0609Guillermina M Luque1https://orcid.org/0000-0002-2745-8236Matias Gomez Elias2Claudia Sanchez Cardenas3Xinran Xu4Jose L de La Vega Beltran5Gabriel Corkidi6Alejandro Linares7Victor Abonza8Aquetzalli Arenas-Hernandez9María DP Ramos-Godinez10Alejandro López-Saavedra11Dario Krapf12https://orcid.org/0000-0001-7607-1954Diego Krapf13Alberto Darszon14Adán Guerrero15Mariano G Buffone16https://orcid.org/0000-0002-7163-6482Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Buenos Aires, ArgentinaInstituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Buenos Aires, ArgentinaInstituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Buenos Aires, ArgentinaDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, MexicoDepartment of Electrical and Computer Engineering and School of Biomedical Engineering, Colorado State University, Fort Collins, United StatesDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, MexicoLaboratorio de Imágenes y Visión por Computadora, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, Mexico; Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, Mexico; Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, MexicoDepartamento de Microscopía Electrónica, Instituto Nacional de Cancerología, Mexico, MexicoUnidad de Aplicaciones Avanzadas en Microscopía, Instituto Nacional de Cancerología Unidad de Investigación Biomédica en Cáncer, Mexico, MexicoUnidad de Aplicaciones Avanzadas en Microscopía, Instituto Nacional de Cancerología Unidad de Investigación Biomédica en Cáncer, Mexico, Mexico; Tecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, Mexico City, MexicoInstituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario (UNR), Rosario, ArgentinaDepartment of Electrical and Computer Engineering and School of Biomedical Engineering, Colorado State University, Fort Collins, United StatesDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, Mexico; Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, MexicoInstituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Buenos Aires, ArgentinaMammalian sperm delve into the female reproductive tract to fertilize the female gamete. The available information about how sperm regulate their motility during the final journey to the fertilization site is extremely limited. In this work, we investigated the structural and functional changes in the sperm flagellum after acrosomal exocytosis (AE) and during the interaction with the eggs. The evidence demonstrates that the double helix actin network surrounding the mitochondrial sheath of the midpiece undergoes structural changes prior to the motility cessation. This structural modification is accompanied by a decrease in diameter of the midpiece and is driven by intracellular calcium changes that occur concomitant with a reorganization of the actin helicoidal cortex. Midpiece contraction occurs in a subset of cells that undergo AE, and live-cell imaging during in vitro fertilization showed that the midpiece contraction is required for motility cessation after fusion is initiated. These findings provide the first evidence of the F-actin network’s role in regulating sperm motility, adapting its function to meet specific cellular requirements during fertilization, and highlighting the broader significance of understanding sperm motility.https://elifesciences.org/articles/93792fusionactinmidpiecemotilitycalcium |
| spellingShingle | Martina Jabloñski Guillermina M Luque Matias Gomez Elias Claudia Sanchez Cardenas Xinran Xu Jose L de La Vega Beltran Gabriel Corkidi Alejandro Linares Victor Abonza Aquetzalli Arenas-Hernandez María DP Ramos-Godinez Alejandro López-Saavedra Dario Krapf Diego Krapf Alberto Darszon Adán Guerrero Mariano G Buffone Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization eLife fusion actin midpiece motility calcium |
| title | Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization |
| title_full | Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization |
| title_fullStr | Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization |
| title_full_unstemmed | Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization |
| title_short | Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization |
| title_sort | reorganization of the flagellum scaffolding induces a sperm standstill during fertilization |
| topic | fusion actin midpiece motility calcium |
| url | https://elifesciences.org/articles/93792 |
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