A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension
Bone marrow mesenchymal stem cells (BMSCs) are intraosseous stem cells, and the effects of tensile strain on BMSC differentiation mediate several bone-related treatments. To study the response of BMSCs under tension, we designed and developed a small cellular tension instrument, iStrain. When iStrai...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/6647651 |
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| author | Yi Zhao Yiping Huang Lingfei Jia Ruoxi Wang Kuang Tan Weiran Li |
| author_facet | Yi Zhao Yiping Huang Lingfei Jia Ruoxi Wang Kuang Tan Weiran Li |
| author_sort | Yi Zhao |
| collection | DOAJ |
| description | Bone marrow mesenchymal stem cells (BMSCs) are intraosseous stem cells, and the effects of tensile strain on BMSC differentiation mediate several bone-related treatments. To study the response of BMSCs under tension, we designed and developed a small cellular tension instrument, iStrain. When iStrain applied tension on BMSCs, these cells exhibited convergence in the alignment direction and lengthening of the cell processes and cell body. Real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting demonstrated that iStrain-mediated cyclic tension promotes the differentiation of BMSCs toward osteogenesis and fibrogenesis. And the mRNA and protein expression of differentiation-related genes changes with the extension of tension time. |
| format | Article |
| id | doaj-art-9bca2d8f6ea34670a8577455d7fde87b |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-9bca2d8f6ea34670a8577455d7fde87b2025-08-20T03:36:57ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/66476516647651A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic TensionYi Zhao0Yiping Huang1Lingfei Jia2Ruoxi Wang3Kuang Tan4Weiran Li5Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Orthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Orthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Orthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of Orthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaBone marrow mesenchymal stem cells (BMSCs) are intraosseous stem cells, and the effects of tensile strain on BMSC differentiation mediate several bone-related treatments. To study the response of BMSCs under tension, we designed and developed a small cellular tension instrument, iStrain. When iStrain applied tension on BMSCs, these cells exhibited convergence in the alignment direction and lengthening of the cell processes and cell body. Real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting demonstrated that iStrain-mediated cyclic tension promotes the differentiation of BMSCs toward osteogenesis and fibrogenesis. And the mRNA and protein expression of differentiation-related genes changes with the extension of tension time.http://dx.doi.org/10.1155/2021/6647651 |
| spellingShingle | Yi Zhao Yiping Huang Lingfei Jia Ruoxi Wang Kuang Tan Weiran Li A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension Stem Cells International |
| title | A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension |
| title_full | A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension |
| title_fullStr | A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension |
| title_full_unstemmed | A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension |
| title_short | A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension |
| title_sort | novel tension machine promotes bone marrow mesenchymal stem cell osteoblastic and fibroblastic differentiation by applying cyclic tension |
| url | http://dx.doi.org/10.1155/2021/6647651 |
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