Hypolipidemic Effect and Mechanism of Bifidobacterium animalis NX-6 on a Zebrafish Model of Hyperlipidemia
Objective: To analyze the characteristics of Bifidobacterium animalis NX-6 and to explore its lipid-lowering mechanism in the model organism zebrafish using metabolomics. Methods: The growth curve of NX-6 was plotted, and the cell morphology was observed using field emission-scanning electron micros...
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China Food Publishing Company
2025-07-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-14-022.pdf |
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| author | HE Yingxi, XU Xinzhu, LIN Shuxian, SHENG Wen, XU Suhua, GUO Lina |
| author_facet | HE Yingxi, XU Xinzhu, LIN Shuxian, SHENG Wen, XU Suhua, GUO Lina |
| author_sort | HE Yingxi, XU Xinzhu, LIN Shuxian, SHENG Wen, XU Suhua, GUO Lina |
| collection | DOAJ |
| description | Objective: To analyze the characteristics of Bifidobacterium animalis NX-6 and to explore its lipid-lowering mechanism in the model organism zebrafish using metabolomics. Methods: The growth curve of NX-6 was plotted, and the cell morphology was observed using field emission-scanning electron microscopy (FE-SEM). Its tolerance to simulated gastrointestinal conditions was assessed. Its safety and potential benefits were evaluated at the genetic level through whole-genome sequencing. Untargeted and targeted metabolomics analysis was conducted based on liquid chromatography-mass spectrometry (LC-MS) to identify metabolites produced by NX-6, focusing on key lipid-lowering metabolites. Hyperlipidemia in zebrafish was induced by feeding a high-fat diet, and Oil Red O staining was used to observe lipid accumulation. This study evaluated the lipid-lowering effect and mechanism of NX-6 and its key metabolites. The hypolipidemic effect of the key metabolite indole-3-acetic acid was evaluated on hyperlipidemic zebrafish. Results: Compared with B. animalis BB12, B. animalis NX-6 demonstrated significantly higher tolerance to artificial gastrointestinal fluids. The genome of NX-6 lacked virulence genes but contained abundant lipid and tryptophan metabolism gene clusters. NX-6 significantly reduced total cholesterol and triglyceride levels in hyperlipidemic zebrafish (P < 0.001). Indole-3-carboxylic acid and 2-indole ketone were significantly increased in the fermentation supernatant of NX-6 compared with BS medium. Following NX-6 treatment, the content of indole-3-carboxylic acid in zebrafish significantly rose (P < 0.001). Indole-3-carboxylic acid notably decreased total cholesterol and triglyceride levels in hyperlipidemic zebrafish (P < 0.001). Conclusion: B. animalis NX-6 reduced lipid accumulation in hyperlipidemic zebrafish through its metabolite indole-3-carboxylic acid. |
| format | Article |
| id | doaj-art-9b98a124ee55420ea18c4f6d3d7c4a8e |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-9b98a124ee55420ea18c4f6d3d7c4a8e2025-08-20T03:25:07ZengChina Food Publishing CompanyShipin Kexue1002-66302025-07-01461420720110.7506/spkx1002-6630-20241218-155Hypolipidemic Effect and Mechanism of Bifidobacterium animalis NX-6 on a Zebrafish Model of HyperlipidemiaHE Yingxi, XU Xinzhu, LIN Shuxian, SHENG Wen, XU Suhua, GUO Lina0(Department of Clinical Nutrition, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China)Objective: To analyze the characteristics of Bifidobacterium animalis NX-6 and to explore its lipid-lowering mechanism in the model organism zebrafish using metabolomics. Methods: The growth curve of NX-6 was plotted, and the cell morphology was observed using field emission-scanning electron microscopy (FE-SEM). Its tolerance to simulated gastrointestinal conditions was assessed. Its safety and potential benefits were evaluated at the genetic level through whole-genome sequencing. Untargeted and targeted metabolomics analysis was conducted based on liquid chromatography-mass spectrometry (LC-MS) to identify metabolites produced by NX-6, focusing on key lipid-lowering metabolites. Hyperlipidemia in zebrafish was induced by feeding a high-fat diet, and Oil Red O staining was used to observe lipid accumulation. This study evaluated the lipid-lowering effect and mechanism of NX-6 and its key metabolites. The hypolipidemic effect of the key metabolite indole-3-acetic acid was evaluated on hyperlipidemic zebrafish. Results: Compared with B. animalis BB12, B. animalis NX-6 demonstrated significantly higher tolerance to artificial gastrointestinal fluids. The genome of NX-6 lacked virulence genes but contained abundant lipid and tryptophan metabolism gene clusters. NX-6 significantly reduced total cholesterol and triglyceride levels in hyperlipidemic zebrafish (P < 0.001). Indole-3-carboxylic acid and 2-indole ketone were significantly increased in the fermentation supernatant of NX-6 compared with BS medium. Following NX-6 treatment, the content of indole-3-carboxylic acid in zebrafish significantly rose (P < 0.001). Indole-3-carboxylic acid notably decreased total cholesterol and triglyceride levels in hyperlipidemic zebrafish (P < 0.001). Conclusion: B. animalis NX-6 reduced lipid accumulation in hyperlipidemic zebrafish through its metabolite indole-3-carboxylic acid.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-14-022.pdfbifidobacterium animalis nx-6; tryptophan metabolites; indole-3-carboxylic acid; zebrafish; lipid-lowering effect |
| spellingShingle | HE Yingxi, XU Xinzhu, LIN Shuxian, SHENG Wen, XU Suhua, GUO Lina Hypolipidemic Effect and Mechanism of Bifidobacterium animalis NX-6 on a Zebrafish Model of Hyperlipidemia Shipin Kexue bifidobacterium animalis nx-6; tryptophan metabolites; indole-3-carboxylic acid; zebrafish; lipid-lowering effect |
| title | Hypolipidemic Effect and Mechanism of Bifidobacterium animalis NX-6 on a Zebrafish Model of Hyperlipidemia |
| title_full | Hypolipidemic Effect and Mechanism of Bifidobacterium animalis NX-6 on a Zebrafish Model of Hyperlipidemia |
| title_fullStr | Hypolipidemic Effect and Mechanism of Bifidobacterium animalis NX-6 on a Zebrafish Model of Hyperlipidemia |
| title_full_unstemmed | Hypolipidemic Effect and Mechanism of Bifidobacterium animalis NX-6 on a Zebrafish Model of Hyperlipidemia |
| title_short | Hypolipidemic Effect and Mechanism of Bifidobacterium animalis NX-6 on a Zebrafish Model of Hyperlipidemia |
| title_sort | hypolipidemic effect and mechanism of bifidobacterium animalis nx 6 on a zebrafish model of hyperlipidemia |
| topic | bifidobacterium animalis nx-6; tryptophan metabolites; indole-3-carboxylic acid; zebrafish; lipid-lowering effect |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-14-022.pdf |
| work_keys_str_mv | AT heyingxixuxinzhulinshuxianshengwenxusuhuaguolina hypolipidemiceffectandmechanismofbifidobacteriumanimalisnx6onazebrafishmodelofhyperlipidemia |