Assessment of the effects of different extraction methods on the phytochemicals, antimicrobial and anticancer activities of Eruca sativa extracts
The present study aimed to investigate the benefits of ultrasonic method of extraction compared to maceration method, on intensifying the phytochemicals, antimicrobial, and cytotoxicity activities of Eruca sativa leaves and sprouts ethanolic extracts. The ultrasonic treatments of E. sativa leaves...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
ResearchersLinks, Ltd
2020-06-01
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| Series: | Novel Research in Microbiology Journal |
| Subjects: | |
| Online Access: | https://nrmj.journals.ekb.eg/article_95325_114db45fdd79f322bac6b0ad4a625f0c.pdf |
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| Summary: | The present study aimed to investigate the benefits of ultrasonic method of extraction compared to maceration
method, on intensifying the phytochemicals, antimicrobial, and cytotoxicity activities of Eruca sativa leaves and
sprouts ethanolic extracts. The ultrasonic treatments of E. sativa leaves and sprout, were tested after 10, 20 and 30
min., whereas, maceration treatments of E. sativa leaves and sprout, were considered after 72 h. Results of Gas
Chromatography-Mass Spectrometry (GC-MS) analysis showed that E. sativa leaves and sprouts are reach
sources of active components such as phenols and flavonoids. Moreover, the sprout macerated extracts showed
higher total antioxidant activity (TAA), total phenolic contents (TPC) and total flavonoids (TFC), compared to
those of the sprout ultrasonic extracts. On the contrary, the ultrasonic extraction of leaves was more efficient than
maceration. Results of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity showed that ultrasonic
treated leaves, sprouts (10 min.), and macerated sprouts (72 h), recorded the lowest effective concentrations
(EC50) of; 2.77, 3.846, and 3.676 mg/100 ml, respectively. The antimicrobial activity of the extracts was assessed
using the well diffusion method. Results showed that ultrasonic treated sprouts (10 min.) and ultrasonic treated
leaves (10 and 30 min.) have better antifungal activity against Aspergillus fumigatus (recording diameter of
inhibition zone of 13 mm) and Candida albicans (recording diameters of inhibition zones of 13 and 11 mm,
respectively). For bacteria, the Staphylococcus aureus was effectively inhibited (recording inhibition zone
diameter of 13 mm) by the ultrasonic treated leaves (20 min.), Bacillus cereus was effectively inhibited with
ultrasonic treated sprouts (20 and 30 min., both recording 13 mm), ultrasonic treated leaves (10 and 20 min.,
recording 14, 13 mm, respectively), and the macerated leaves (72 h, recording 13 mm). Salmonella typhimurium
recorded (13 mm) with ultrasonic treated leaves (10 min.), and (12 mm) with macerated sprouts. Results of the
cytotoxic potency demonstrated high activity of the macerated sprouts on HepG2 cell lines, whereas the
macerated sprouts and ultrasonic treated leaves (30 min.) presented high efficacy on the HT-29 cells. Thus we
concluded that extracts of E. sativa leaves and sprouts produced using different extraction methods, have a
substantial beneficial antioxidant, antimicrobial and anticancer activities. |
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| ISSN: | 2537-0286 2537-0294 |