Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries
Two methods have been described to recover oocytes from equine follicles in excised ovaries: aspiration and scraping. Aim of this work was to develop an effective method for collecting equine oocytes using Tuohy needle and comparing this technique to aspiration and scraping, with or without tunica a...
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| Language: | English |
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Wiley
2010-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.4061/2010/102591 |
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| author | F. Cremonesi K. Anderson A. Lange-Consiglio |
| author_facet | F. Cremonesi K. Anderson A. Lange-Consiglio |
| author_sort | F. Cremonesi |
| collection | DOAJ |
| description | Two methods have been described to recover oocytes from equine follicles in excised ovaries: aspiration and scraping. Aim of this work was to develop an effective method for collecting equine oocytes using Tuohy needle and comparing this technique to aspiration and scraping, with or without tunica albuginea removal. This hollow hypodermic needle, usually employed for inserting epidural catheters, is designed with a slightly curved tip, shaped similar to a small curette. In unpeeled ovaries, the recovery rates of Tuohy needle group was higher (𝑃<.05) than in the 16 g needle aspiration and in the scraped ovaries (57% versus 36% and 47%) while the rate of cumulus-intact oocytes was higher than aspiration (46.9% versus 39.36%) but lower than scraping (46.97%) (𝑃<.001). In unpeeled ovaries there was significant difference in maturation rate of oocytes recovered by Tuohy needle in respect to peeled ovaries (58.54% versus 50.17%, resp.). Combination of aspiration and scraping by Tuohy needle allows a faster and reliable collection of oocytes suitable for horse IVM. |
| format | Article |
| id | doaj-art-9b94b3a436884cb2a6490905a2c6b352 |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-9b94b3a436884cb2a6490905a2c6b3522025-02-03T05:50:53ZengWileyVeterinary Medicine International2042-00482010-01-01201010.4061/2010/102591102591Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare OvariesF. Cremonesi0K. Anderson1A. Lange-Consiglio2Università degli Studi di Milano, Large Animal Hospital, Reproduction Unit, via dell'Università 6, 26900 Lodi, ItalyUniversity of Wisconsin-River Falls, River falls, WI 54022, USAUniversità degli Studi di Milano, Large Animal Hospital, Reproduction Unit, via dell'Università 6, 26900 Lodi, ItalyTwo methods have been described to recover oocytes from equine follicles in excised ovaries: aspiration and scraping. Aim of this work was to develop an effective method for collecting equine oocytes using Tuohy needle and comparing this technique to aspiration and scraping, with or without tunica albuginea removal. This hollow hypodermic needle, usually employed for inserting epidural catheters, is designed with a slightly curved tip, shaped similar to a small curette. In unpeeled ovaries, the recovery rates of Tuohy needle group was higher (𝑃<.05) than in the 16 g needle aspiration and in the scraped ovaries (57% versus 36% and 47%) while the rate of cumulus-intact oocytes was higher than aspiration (46.9% versus 39.36%) but lower than scraping (46.97%) (𝑃<.001). In unpeeled ovaries there was significant difference in maturation rate of oocytes recovered by Tuohy needle in respect to peeled ovaries (58.54% versus 50.17%, resp.). Combination of aspiration and scraping by Tuohy needle allows a faster and reliable collection of oocytes suitable for horse IVM.http://dx.doi.org/10.4061/2010/102591 |
| spellingShingle | F. Cremonesi K. Anderson A. Lange-Consiglio Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries Veterinary Medicine International |
| title | Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries |
| title_full | Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries |
| title_fullStr | Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries |
| title_full_unstemmed | Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries |
| title_short | Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries |
| title_sort | efficacy of tuohy needle in oocytes collection from excised mare ovaries |
| url | http://dx.doi.org/10.4061/2010/102591 |
| work_keys_str_mv | AT fcremonesi efficacyoftuohyneedleinoocytescollectionfromexcisedmareovaries AT kanderson efficacyoftuohyneedleinoocytescollectionfromexcisedmareovaries AT alangeconsiglio efficacyoftuohyneedleinoocytescollectionfromexcisedmareovaries |