Phase I study of a recombinant attenuated oncolytic virus, MEDI5395 (NDV–GM-CSF), administered systemically in combination with durvalumab in patients with advanced solid tumors

Background MEDI5395 is a recombinant attenuated Newcastle disease virus engineered to express a human granulocyte-macrophage colony-stimulating factor transgene. Preclinically, MEDI5395 demonstrated broad oncolytic activity, augmented by concomitant programmed cell death-1/programmed cell death liga...

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Main Authors: Dmitriy Zamarin, Diwakar Davar, Adel Samson, Kevin J Harrington, Siddharth Sheth, Eric Tu, Mitesh J Borad, Grace K Dy, Evanthia Galanis, Fernanda Arnaldez, Sandip P Patel, Nicholas Durham, Benedito A Carneiro, Sonia Agrawal, Zhongying Chen, Chunling Fan, Maozhen Gong, Jenny Burton, Kevin Laubscher
Format: Article
Language:English
Published: BMJ Publishing Group 2024-11-01
Series:Journal for ImmunoTherapy of Cancer
Online Access:https://jitc.bmj.com/content/12/11/e009336.full
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author Dmitriy Zamarin
Diwakar Davar
Adel Samson
Kevin J Harrington
Siddharth Sheth
Eric Tu
Mitesh J Borad
Grace K Dy
Evanthia Galanis
Fernanda Arnaldez
Sandip P Patel
Nicholas Durham
Benedito A Carneiro
Sonia Agrawal
Zhongying Chen
Chunling Fan
Maozhen Gong
Jenny Burton
Kevin Laubscher
author_facet Dmitriy Zamarin
Diwakar Davar
Adel Samson
Kevin J Harrington
Siddharth Sheth
Eric Tu
Mitesh J Borad
Grace K Dy
Evanthia Galanis
Fernanda Arnaldez
Sandip P Patel
Nicholas Durham
Benedito A Carneiro
Sonia Agrawal
Zhongying Chen
Chunling Fan
Maozhen Gong
Jenny Burton
Kevin Laubscher
author_sort Dmitriy Zamarin
collection DOAJ
description Background MEDI5395 is a recombinant attenuated Newcastle disease virus engineered to express a human granulocyte-macrophage colony-stimulating factor transgene. Preclinically, MEDI5395 demonstrated broad oncolytic activity, augmented by concomitant programmed cell death-1/programmed cell death ligand-1 (PD-L1) axis blockade. Durvalumab is an anti-PD-L1 immune checkpoint inhibitor approved for the treatment of various solid tumors. We describe the results of the first-in-human study combining intravenous MEDI5395 with durvalumab in patients with advanced solid tumors.Methods This phase I, open-label, multicenter, dose-escalation, dose-expansion study recruited adult patients with advanced solid tumors, who had relapsed or were refractory or intolerant to ≥1 prior line of standard treatment. MEDI5395 was administered intravenously as six doses over 15–18 days. The dose-escalation phase assessed four-dose levels (108, 109, 1010, 1011 focus forming units (FFU)) of MEDI5395, with sequential or delayed durvalumab. Durvalumab 1500 mg was administered intravenously every 4 weeks up to 2 years. The dose-expansion phase was not initiated. The primary objectives were to evaluate safety and tolerability, dose-limiting toxicities (DLTs) and the dose and schedule of MEDI5395 plus durvalumab administration. Secondary objectives included the assessment of the efficacy, pharmacokinetics, pharmacodynamics, and immunogenicity of MEDI5395.Results 39 patients were treated with MEDI5395; 36 patients also received durvalumab. All 39 patients experienced ≥1 treatment-emergent adverse event (TEAE), most commonly fatigue (61.5%), nausea (53.8%) and chills (51.3%). Grade 3–4 TEAEs occurred in 27 (69.2%) patients; these were deemed MEDI5395-related in 12 (30.8%) patients. Two patients experienced a DLT, and the maximum tolerated dose of MEDI5395 with sequential and delayed durvalumab at study termination was 1011 and 1010 FFU, respectively. Four patients (10.3%) achieved a partial response (PR). Patients with PR or stable disease tended to have higher baseline PD-L1 and CD8+ levels in their tumor tissue. A tendency to dose-dependent pharmacokinetics of the viral genome was observed in whole blood and a tendency to dose-dependent viral shedding was observed in saliva and urine. Neutralizing antibodies were observed in all patients but did not appear to impact efficacy negatively.Conclusion This study demonstrates the feasibility, safety and preliminary efficacy of MEDI5395 with durvalumab in patients with advanced solid tumors.Trial registration number NCT03889275
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spelling doaj-art-9b8ca43a8d514a34aab835d920738e152025-08-20T01:52:21ZengBMJ Publishing GroupJournal for ImmunoTherapy of Cancer2051-14262024-11-01121110.1136/jitc-2024-009336Phase I study of a recombinant attenuated oncolytic virus, MEDI5395 (NDV–GM-CSF), administered systemically in combination with durvalumab in patients with advanced solid tumorsDmitriy Zamarin0Diwakar Davar1Adel Samson2Kevin J Harrington3Siddharth Sheth4Eric Tu5Mitesh J Borad6Grace K Dy7Evanthia Galanis8Fernanda Arnaldez9Sandip P Patel10Nicholas Durham11Benedito A Carneiro12Sonia Agrawal13Zhongying Chen14Chunling Fan15Maozhen Gong16Jenny Burton17Kevin Laubscher18Early Drug Development, Memorial Sloan Kettering Cancer Center, New York, New York, USAUPMC Hillman Cancer Center, Pittsburgh, Pennsylvania, USALeeds Institute of Medical Research at St. James’s, University of Leeds, Leeds, UKDivision of Radiotherapy and Imaging, The Institute of Cancer Research / The Royal Marsden NIHR Biomedical Research Centre, London, UKDivision of Hematology/Oncology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USATranslational Medicine, Cell Therapy and Oncolytic Viruses, BioPharmaceuticals R&D, AstraZeneca R&D Gaithersburg, Gaithersburg, Maryland, USAMayo Clinic, Phoenix, Arizona, USA5Roswell Park Comprehensive Cancer Center, Buffalo, NY, USAMayo Clinic Rochester, Rochester, Minnesota, USAClinical Development, Oncology R&D, AstraZeneca R&D Gaithersburg, Gaithersburg, Maryland, USAMoores Cancer Center, University of California San Diego, La Jolla, California, USATranslational Medicine, Cell Therapy and Oncolytic Viruses, BioPharmaceuticals R&D, AstraZeneca R&D Gaithersburg, Gaithersburg, Maryland, USALifespan Cancer Institute, Legorreta Cancer Institute at Brown University, Providence, Rhode Island, USAOncology Data Science, Research and Early Development, Oncology R&D, AstraZeneca R&D Gaithersburg, Gaithersburg, Maryland, USAIntegrated Bioanalysis, Clinical Pharmacology and Safety Sciences (CPSS), BioPharmaceuticals R&D, AstraZeneca R&D Gaithersburg, Gaithersburg, Maryland, USAClinical Pharmacology and Quantitative Pharmacology, Clinical Pharmacology and Safety Sciences (CPSS), BioPharmaceuticals R&D, AstraZeneca R&D Gaithersburg, Gaithersburg, Maryland, USAAstraZeneca R&D Gaithersburg, Gaithersburg, Maryland, USAOncology R&D, AstraZeneca PLC, Cambridge, Cambridgeshire, UKOncology Data Science, Research and Early Development, Oncology R&D, AstraZeneca R&D Gaithersburg, Gaithersburg, Maryland, USABackground MEDI5395 is a recombinant attenuated Newcastle disease virus engineered to express a human granulocyte-macrophage colony-stimulating factor transgene. Preclinically, MEDI5395 demonstrated broad oncolytic activity, augmented by concomitant programmed cell death-1/programmed cell death ligand-1 (PD-L1) axis blockade. Durvalumab is an anti-PD-L1 immune checkpoint inhibitor approved for the treatment of various solid tumors. We describe the results of the first-in-human study combining intravenous MEDI5395 with durvalumab in patients with advanced solid tumors.Methods This phase I, open-label, multicenter, dose-escalation, dose-expansion study recruited adult patients with advanced solid tumors, who had relapsed or were refractory or intolerant to ≥1 prior line of standard treatment. MEDI5395 was administered intravenously as six doses over 15–18 days. The dose-escalation phase assessed four-dose levels (108, 109, 1010, 1011 focus forming units (FFU)) of MEDI5395, with sequential or delayed durvalumab. Durvalumab 1500 mg was administered intravenously every 4 weeks up to 2 years. The dose-expansion phase was not initiated. The primary objectives were to evaluate safety and tolerability, dose-limiting toxicities (DLTs) and the dose and schedule of MEDI5395 plus durvalumab administration. Secondary objectives included the assessment of the efficacy, pharmacokinetics, pharmacodynamics, and immunogenicity of MEDI5395.Results 39 patients were treated with MEDI5395; 36 patients also received durvalumab. All 39 patients experienced ≥1 treatment-emergent adverse event (TEAE), most commonly fatigue (61.5%), nausea (53.8%) and chills (51.3%). Grade 3–4 TEAEs occurred in 27 (69.2%) patients; these were deemed MEDI5395-related in 12 (30.8%) patients. Two patients experienced a DLT, and the maximum tolerated dose of MEDI5395 with sequential and delayed durvalumab at study termination was 1011 and 1010 FFU, respectively. Four patients (10.3%) achieved a partial response (PR). Patients with PR or stable disease tended to have higher baseline PD-L1 and CD8+ levels in their tumor tissue. A tendency to dose-dependent pharmacokinetics of the viral genome was observed in whole blood and a tendency to dose-dependent viral shedding was observed in saliva and urine. Neutralizing antibodies were observed in all patients but did not appear to impact efficacy negatively.Conclusion This study demonstrates the feasibility, safety and preliminary efficacy of MEDI5395 with durvalumab in patients with advanced solid tumors.Trial registration number NCT03889275https://jitc.bmj.com/content/12/11/e009336.full
spellingShingle Dmitriy Zamarin
Diwakar Davar
Adel Samson
Kevin J Harrington
Siddharth Sheth
Eric Tu
Mitesh J Borad
Grace K Dy
Evanthia Galanis
Fernanda Arnaldez
Sandip P Patel
Nicholas Durham
Benedito A Carneiro
Sonia Agrawal
Zhongying Chen
Chunling Fan
Maozhen Gong
Jenny Burton
Kevin Laubscher
Phase I study of a recombinant attenuated oncolytic virus, MEDI5395 (NDV–GM-CSF), administered systemically in combination with durvalumab in patients with advanced solid tumors
Journal for ImmunoTherapy of Cancer
title Phase I study of a recombinant attenuated oncolytic virus, MEDI5395 (NDV–GM-CSF), administered systemically in combination with durvalumab in patients with advanced solid tumors
title_full Phase I study of a recombinant attenuated oncolytic virus, MEDI5395 (NDV–GM-CSF), administered systemically in combination with durvalumab in patients with advanced solid tumors
title_fullStr Phase I study of a recombinant attenuated oncolytic virus, MEDI5395 (NDV–GM-CSF), administered systemically in combination with durvalumab in patients with advanced solid tumors
title_full_unstemmed Phase I study of a recombinant attenuated oncolytic virus, MEDI5395 (NDV–GM-CSF), administered systemically in combination with durvalumab in patients with advanced solid tumors
title_short Phase I study of a recombinant attenuated oncolytic virus, MEDI5395 (NDV–GM-CSF), administered systemically in combination with durvalumab in patients with advanced solid tumors
title_sort phase i study of a recombinant attenuated oncolytic virus medi5395 ndv gm csf administered systemically in combination with durvalumab in patients with advanced solid tumors
url https://jitc.bmj.com/content/12/11/e009336.full
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