Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves upon Exposure to Powdery Mildew
Powdery mildew affecting European hazelnut Corylus avellana L. trees in Turkey is caused by the obligate biotrophic fungus Erysiphe corylacearum. This fungal disease causes significant economic losses by reducing the yield and quality of hazelnuts. Loss-of-function mutations in the mildew resistance...
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Hasan Eleroğlu
2022-04-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/4686 |
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| author | Ulku Baykal Kadriye Özcan |
| author_facet | Ulku Baykal Kadriye Özcan |
| author_sort | Ulku Baykal |
| collection | DOAJ |
| description | Powdery mildew affecting European hazelnut Corylus avellana L. trees in Turkey is caused by the obligate biotrophic fungus Erysiphe corylacearum. This fungal disease causes significant economic losses by reducing the yield and quality of hazelnuts. Loss-of-function mutations in the mildew resistance locus o (MLO) gene family of many plants confer high levels of broad-spectrum resistance to powdery mildew. The proteins encoded by the genes at the MLO locus are divided into approximately seven different conserved clades. Among them, the phylogenetic clade V has been shown to be involved in PM susceptibility, as inactivation of these genes leads to long-term disease resistance in dicotyledons. In this study, we examined the temporal expression pattern of three hazelnut MLO genes, previously identified as clade V, in response to powdery mildew infection in C. avellana cv. Tombul. Leaves are the main tissue affected by the powdery mildew pathogen in hazelnut plants. Analysis of MLO expression in hazelnut leaves showed that CavMLO2 and CavMLO6 were significantly upregulated after challenge with E. corylacearum, providing preliminary evidence that they may be involved in PM susceptibility. Thus, these results present a basis for the isolation and use of relevant genes in plant breeding for disease resistance. Moreover, expression profiles of the clade V MLO genes are also important to identify candidate genes that need to be silenced or edited for future molecular studies to obtain resistant hazelnut varieties. |
| format | Article |
| id | doaj-art-9b805b45d20c4cada1c4b08293da7016 |
| institution | Kabale University |
| issn | 2148-127X |
| language | English |
| publishDate | 2022-04-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-9b805b45d20c4cada1c4b08293da70162025-08-20T03:36:57ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2022-04-0110459561210.24925/turjaf.v10i4.595-612.46862438Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves upon Exposure to Powdery MildewUlku Baykal0Kadriye Özcan1Department of Genetics and Bioengineering, Faculty of Engineering, Giresun University, Güre, 28200 GiresunDepartment of Genetics and Bioengineering, Faculty of Engineering, Giresun University, Güre, 28200 GiresunPowdery mildew affecting European hazelnut Corylus avellana L. trees in Turkey is caused by the obligate biotrophic fungus Erysiphe corylacearum. This fungal disease causes significant economic losses by reducing the yield and quality of hazelnuts. Loss-of-function mutations in the mildew resistance locus o (MLO) gene family of many plants confer high levels of broad-spectrum resistance to powdery mildew. The proteins encoded by the genes at the MLO locus are divided into approximately seven different conserved clades. Among them, the phylogenetic clade V has been shown to be involved in PM susceptibility, as inactivation of these genes leads to long-term disease resistance in dicotyledons. In this study, we examined the temporal expression pattern of three hazelnut MLO genes, previously identified as clade V, in response to powdery mildew infection in C. avellana cv. Tombul. Leaves are the main tissue affected by the powdery mildew pathogen in hazelnut plants. Analysis of MLO expression in hazelnut leaves showed that CavMLO2 and CavMLO6 were significantly upregulated after challenge with E. corylacearum, providing preliminary evidence that they may be involved in PM susceptibility. Thus, these results present a basis for the isolation and use of relevant genes in plant breeding for disease resistance. Moreover, expression profiles of the clade V MLO genes are also important to identify candidate genes that need to be silenced or edited for future molecular studies to obtain resistant hazelnut varieties.http://www.agrifoodscience.com/index.php/TURJAF/article/view/4686hazelnutcorylus avellanamildew resistance locus o (mlo)powdery mildewsemi-quantitative pcr |
| spellingShingle | Ulku Baykal Kadriye Özcan Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves upon Exposure to Powdery Mildew Turkish Journal of Agriculture: Food Science and Technology hazelnut corylus avellana mildew resistance locus o (mlo) powdery mildew semi-quantitative pcr |
| title | Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves upon Exposure to Powdery Mildew |
| title_full | Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves upon Exposure to Powdery Mildew |
| title_fullStr | Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves upon Exposure to Powdery Mildew |
| title_full_unstemmed | Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves upon Exposure to Powdery Mildew |
| title_short | Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves upon Exposure to Powdery Mildew |
| title_sort | analysis of clade v mlo gene expressions in hazelnut leaves upon exposure to powdery mildew |
| topic | hazelnut corylus avellana mildew resistance locus o (mlo) powdery mildew semi-quantitative pcr |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/4686 |
| work_keys_str_mv | AT ulkubaykal analysisofcladevmlogeneexpressionsinhazelnutleavesuponexposuretopowderymildew AT kadriyeozcan analysisofcladevmlogeneexpressionsinhazelnutleavesuponexposuretopowderymildew |