Quantification of matrix metalloproteinases MMP-8 and MMP-9 in gingival overgrowth
Background: Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in extracellular matrix remodeling of all body tissues, including oral tissues such as gingival tissue. Expression levels of MMPs are widely studied as important biomarkers for explaining the biochemical mechanisms and evo...
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Springer
2021-07-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905220307422 |
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| author | Jennifer Orozco-Páez Erika Rodríguez-Cavallo Antonio Díaz-Caballero Darío Méndez-Cuadro |
| author_facet | Jennifer Orozco-Páez Erika Rodríguez-Cavallo Antonio Díaz-Caballero Darío Méndez-Cuadro |
| author_sort | Jennifer Orozco-Páez |
| collection | DOAJ |
| description | Background: Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in extracellular matrix remodeling of all body tissues, including oral tissues such as gingival tissue. Expression levels of MMPs are widely studied as important biomarkers for explaining the biochemical mechanisms and evolution of many oral diseases. Objective: Demonstrate the sensitivity, reproducibility, repeatability, and robustness of the dot blot assay for the relative quantification of MMP-8 and MMP-9 expression levels in patients with GO associated with orthodontic treatment. Methods: A validated dot blot assay was used to compare the relative expression levels of MMP-8 and MMP-9 in gingival samples. Methodological variability, reproducibility, sensitivity and robustness were determined with the use of control samples from healthy donors (G1). Next, expression levels were measured in gingival tissue from patients with mild and moderate gingival overgrowth associated with orthodontic treatment (G3 and G4) and patients without gingival overgrowth but with a history of using orthodontic appliances (G2). Results: Dot blot assay demonstrated that MMP-8 and MMP-9 expression levels were higher in patients with gingival overgrowth and distinguished those with moderate clinical grade (G4) from those with mild overgrowth (G3). In addition, patients with a history of orthodontic treatment showed similar expression levels to the control group two years after removing orthodontic appliances. Conclusions: With the assay used, we were able to detect differences in MMP-8 and MMP-9 expression in patients with different levels of severity of gingival overgrowth. Dot blot could be used to measure MMPs during the onset and progression of gingival overgrowth. |
| format | Article |
| id | doaj-art-9b6827c7e13c427eacbb30d70fac2a61 |
| institution | OA Journals |
| issn | 1013-9052 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj-art-9b6827c7e13c427eacbb30d70fac2a612025-08-20T01:56:06ZengSpringerSaudi Dental Journal1013-90522021-07-0133526026710.1016/j.sdentj.2020.07.001Quantification of matrix metalloproteinases MMP-8 and MMP-9 in gingival overgrowthJennifer Orozco-Páez0Erika Rodríguez-Cavallo1Antonio Díaz-Caballero2Darío Méndez-Cuadro3Analytical Chemistry and Biomedicine Group, University of Cartagena, Cartagena, ColombiaPharmacy Department, Faculty of Pharmaceutical Sciences, University of Cartagena, Leader of Analytical Chemistry and Biomedicine Research Group, Cartagena, Colombia; Corresponding authors at: University of Cartagena, Faculty of Exact and Natural Sciences, Campus of San Pablo, Analytical Chemistry and Biomedicine Group, First Floor No.109, Postal Code 13001 Cartagena de Indias, Colombia (Darío Méndez-Cuadro). University of Cartagena, Faculty of Pharmaceutical Sciences, Campus of Zaragocilla ancient building Cread, Analytical Chemistry and Biomedicine Group, First Floor No.103, Postal Code 13001 Cartagena de Indias, Colombia (Erika Rodríguez-Cavallo).Oral Medicine Department, Faculty of Dentistry, University of Cartagena, Leader of GITOUC Research Group, Cartagena, ColombiaDepartment of Biology, Faculty of Exact and Natural Sciences, University of Cartagena, Leader of Analytical Chemistry and Biomedicine Research Group, Cartagena, Colombia; Corresponding authors at: University of Cartagena, Faculty of Exact and Natural Sciences, Campus of San Pablo, Analytical Chemistry and Biomedicine Group, First Floor No.109, Postal Code 13001 Cartagena de Indias, Colombia (Darío Méndez-Cuadro). University of Cartagena, Faculty of Pharmaceutical Sciences, Campus of Zaragocilla ancient building Cread, Analytical Chemistry and Biomedicine Group, First Floor No.103, Postal Code 13001 Cartagena de Indias, Colombia (Erika Rodríguez-Cavallo).Background: Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in extracellular matrix remodeling of all body tissues, including oral tissues such as gingival tissue. Expression levels of MMPs are widely studied as important biomarkers for explaining the biochemical mechanisms and evolution of many oral diseases. Objective: Demonstrate the sensitivity, reproducibility, repeatability, and robustness of the dot blot assay for the relative quantification of MMP-8 and MMP-9 expression levels in patients with GO associated with orthodontic treatment. Methods: A validated dot blot assay was used to compare the relative expression levels of MMP-8 and MMP-9 in gingival samples. Methodological variability, reproducibility, sensitivity and robustness were determined with the use of control samples from healthy donors (G1). Next, expression levels were measured in gingival tissue from patients with mild and moderate gingival overgrowth associated with orthodontic treatment (G3 and G4) and patients without gingival overgrowth but with a history of using orthodontic appliances (G2). Results: Dot blot assay demonstrated that MMP-8 and MMP-9 expression levels were higher in patients with gingival overgrowth and distinguished those with moderate clinical grade (G4) from those with mild overgrowth (G3). In addition, patients with a history of orthodontic treatment showed similar expression levels to the control group two years after removing orthodontic appliances. Conclusions: With the assay used, we were able to detect differences in MMP-8 and MMP-9 expression in patients with different levels of severity of gingival overgrowth. Dot blot could be used to measure MMPs during the onset and progression of gingival overgrowth.http://www.sciencedirect.com/science/article/pii/S1013905220307422ImmunoblottingDot blot assayGingival overgrowthMatrix metalloproteinases |
| spellingShingle | Jennifer Orozco-Páez Erika Rodríguez-Cavallo Antonio Díaz-Caballero Darío Méndez-Cuadro Quantification of matrix metalloproteinases MMP-8 and MMP-9 in gingival overgrowth Saudi Dental Journal Immunoblotting Dot blot assay Gingival overgrowth Matrix metalloproteinases |
| title | Quantification of matrix metalloproteinases MMP-8 and MMP-9 in gingival overgrowth |
| title_full | Quantification of matrix metalloproteinases MMP-8 and MMP-9 in gingival overgrowth |
| title_fullStr | Quantification of matrix metalloproteinases MMP-8 and MMP-9 in gingival overgrowth |
| title_full_unstemmed | Quantification of matrix metalloproteinases MMP-8 and MMP-9 in gingival overgrowth |
| title_short | Quantification of matrix metalloproteinases MMP-8 and MMP-9 in gingival overgrowth |
| title_sort | quantification of matrix metalloproteinases mmp 8 and mmp 9 in gingival overgrowth |
| topic | Immunoblotting Dot blot assay Gingival overgrowth Matrix metalloproteinases |
| url | http://www.sciencedirect.com/science/article/pii/S1013905220307422 |
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